驴抗山羊IgG H&L (Alexa Fluor® 405)预吸附二抗(ab175665)
Key features and details
- Donkey Anti-Goat IgG H&L (Alexa Fluor® 405) preadsorbed
- Conjugation: Alexa Fluor® 405. Ex: 402nm, Em: 421nm
- Host species: Donkey
- Isotype: IgG
- Suitable for: IHC-Fr, ICC/IF, ELISA, IHC-P, Flow Cyt
Related conjugates and formulations
概述
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产品名称
驴抗山羊IgG H&L (Alexa Fluor® 405)预吸附二抗
参阅全部 IgG 二抗 -
宿主
Donkey -
靶标种属
Goat -
特异性
By immunoelectrophoresis and ELISA this antibody reacts specifically with goat IgG and with light chains common to other goat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to chicken, human, mouse, pig, rabbit and rat IgG was detected. This antibody may cross react with IgG from other species. -
经测试应用
适用于: IHC-Fr, ICC/IF, ELISA, IHC-P, Flow Cytmore details -
最小交叉反应
Chicken, Human, Mouse, Pig, Rabbit, Rat more details -
免疫原
Full length protein corresponding to Goat IgG.
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偶联物
Alexa Fluor® 405. Ex: 402nm, Em: 421nm
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark. -
存储溶液
Preservative: 0.02% Sodium azide
Constituents: 23% Glycerol (glycerin, glycerine), PBS, 1% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
常规说明
We recommend storage time at 4°C should be minimal, since this may affect the signal strength.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab175665于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-Fr | (1) |
Use at an assay dependent concentration.
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| ICC/IF |
1/200 - 1/1000.
We recommend the use of a dedicated 405 filter for optimal results not the DAPI filter. The DAPI filter may not excite until the maximum emission peaks of Alexa Fluor® 405 dye (see difference below) |
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| ELISA |
Use at an assay dependent concentration.
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| IHC-P |
Use at an assay dependent concentration.
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| Flow Cyt |
1/2000.
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| 说明 |
|---|
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IHC-Fr
Use at an assay dependent concentration. |
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ICC/IF
1/200 - 1/1000. We recommend the use of a dedicated 405 filter for optimal results not the DAPI filter. The DAPI filter may not excite until the maximum emission peaks of Alexa Fluor® 405 dye (see difference below) |
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ELISA
Use at an assay dependent concentration. |
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IHC-P
Use at an assay dependent concentration. |
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Flow Cyt
1/2000. |
图片
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Immunocytochemistry/ Immunofluorescence - Donkey Anti-Goat IgG H&L (Alexa Fluor® 405) preadsorbed (ab175665)
ICC/IF image of ab7291 stained HeLa cells. The cells were 100% methanol fixed (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then incubated in 1%BSA / 0.3M glycine in 0.1% PBS-Tween for 1h to block non-specific protein-protein interactions. The cells were then incubated with the primary antibody (ab7291, 1µg/ml) overnight at +4°C. Ab98800, goat anti-mouse IgG, was then added as a secondary bridging antibody, at 1/250 dilution for 1h. Ab175665 Alexa Fluor® 405 Donkey polyclonal Secondary Antibody to Goat IgG - H&L pre-adsorbed (blue) was then used at 1µg/ml for 1h as a tertiary antibody. DRAQ5™ (ab108410) was used to stain the cell nuclei (red) at a concentration of 1.25µM.
The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.
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Immunohistochemistry (Frozen sections) - Donkey Anti-Goat IgG H&L (Alexa Fluor® 405) preadsorbed (ab175665)This image is courtesy of an Abreview submitted by Bryan NiedenbergerPostnatal day 6 mouse testes were fixed with 4% paraformaldehyde. Tissue was embedded in O.C.T. and frozen. 5 micron sections were cut and transferred to slides. Sections were permeabilized with 0.1% Triton X-100 in PBS, and blocked with 3% BSA in 0.1% Triton X-100 + PBS. Sections were incubated with either (A) no primary antibody or (B) Goat Anti-DDX4 at a 1/800 dilution for 1 h at RT. Sections were then washed 3X with 0.1% Triton X-100 in PBS and Donkey Anti-Goat IgG H&L (Alexa Fluor® 405) (ab175665) applied at a 1/500 dilution. Sections were then mounted after washing 3X with 0.1% Triton X-100 in PBS.
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Alexa Fluor® - Donkey Anti-Goat IgG H&L (Alexa Fluor® 405) preadsorbed (ab175665)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (9)
ab175665 被引用在 9 文献中.
- Koba S et al. A brainstem monosynaptic excitatory pathway that drives locomotor activities and sympathetic cardiovascular responses. Nat Commun 13:5079 (2022). PubMed: 36038592
- Sharon A et al. Inflammatory Foreign Body Response Induced by Neuro-Implants in Rat Cortices Depleted of Resident Microglia by a CSF1R Inhibitor and Its Implications. Front Neurosci 15:646914 (2021). PubMed: 33841088
- Ronzano R et al. Proximal and distal spinal neurons innervating multiple synergist and antagonist motor pools. Elife 10:N/A (2021). PubMed: 34727018
- Huang SH et al. Immunohistological and Ultrastructural Study of the Inflammatory Response to Perforated Polyimide Cortical Implants: Mechanisms Underlying Deterioration of Electrophysiological Recording Quality. Front Neurosci 14:926 (2020). PubMed: 32982683
- Kober-Hasslacher M et al. c-Rel gain in B cells drives germinal center reactions and autoantibody production. J Clin Invest 130:3270-3286 (2020). PubMed: 32191641
- Scott RW et al. Hic1 Defines Quiescent Mesenchymal Progenitor Subpopulations with Distinct Functions and Fates in Skeletal Muscle Regeneration. Cell Stem Cell 25:797-813.e9 (2019). PubMed: 31809738
- Park HE et al. Scalable and Isotropic Expansion of Tissues with Simply Tunable Expansion Ratio. Adv Sci (Weinh) 6:1901673 (2019). PubMed: 31763149
- Strubberg AM et al. Cftr Modulates Wnt/ß-Catenin Signaling and Stem Cell Proliferation in Murine Intestine. Cell Mol Gastroenterol Hepatol 5:253-271 (2018). PubMed: 29675451
- Wilson DE et al. GABAergic Neurons in Ferret Visual Cortex Participate in Functionally Specific Networks. Neuron 93:1058-1065.e4 (2017). PubMed: 28279352