驴抗山羊IgG H&L (HRP)预吸附二抗

• WB

Supplier Data

Western blot - Donkey Anti-Goat IgG H&L (HRP) preadsorbed (AB7125)

* "Doublecortin microtubule affinity is regulated by a balance of kinase and phosphatase activity at the leading edge of migrating neurons." Schaar B.T., Kinoshita K., McConnell S.K. Neuron 41 : 203213 (2004).

Minimal cortex defined : small portion of cortex removed prior to lysis.

All lanes:

Western blot - Anti-Doublecortin antibody (<a href='/products/primary-antibodies/doublecortin-antibody-ab235153'>ab235153</a>) at 1/1000 dilution

Lane 1:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate at 15 µg

Lane 2:

Post-natal rat pup whole brain lysate (PND2-6)* at 15 µg

Lane 3:

Rat pup PND2-6 - minimal cortex brain lysate at 15 µg

Lane 4:

Rat pup PND2-6 – minimal cortex brain lysate at 35 µg

Secondary

All lanes:

Western blot - Donkey Anti-Goat IgG H&L (HRP) preadsorbed (ab7125) at 1/40000 dilution

Predicted band size: 49 kDa

Observed band size: 45 kDa

false

Exposure time: 15s

• WB

Unknown

Western blot - Donkey Anti-Goat IgG H&L (HRP) preadsorbed (AB7125)

ab7125 was used at dilution 1/10000 with the primary antibody ab37917 in WB. See the review on ab37917.

Secondary

All lanes:

Western blot - Donkey Anti-Goat IgG H&L (HRP) preadsorbed (ab7125)

false

• WB

Supplier Data

Western blot - Donkey Anti-Goat IgG H&L (HRP) preadsorbed (AB7125)

All lanes:

Western blot - Anti-Doublecortin antibody (<a href='/products/primary-antibodies/doublecortin-antibody-ab235153'>ab235153</a>) at 1/1000 dilution

Lane 1:

Doublecortin (human) overexpressing HEK-293T lysate C-Myc/DDK tagged

Lane 2:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate

Lane 3:

Adult mouse normal brain tissue lysate

Lane 4:

Adult rat normal brain tissue lysate

Secondary

All lanes:

Western blot - Donkey Anti-Goat IgG H&L (HRP) preadsorbed (ab7125) at 1/40000 dilution

Predicted band size: 49 kDa

Observed band size: 35 kDa,48 kDa

false

Exposure time: 15s