AB150131

Donkey Anti-Goat IgG H&L (Alexa Fluor® 647)

Name: 驴抗山羊IgG H&L (Alexa Fluor® 647) (ab150131)| Abcam中文官网
Brand: Abcam Limited
SKU: AB150131
Price: 1242 CNY
Availability: InStock

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(95 Publications)

Donkey Anti-Goat IgG H&L (Alexa Fluor® 647) is a secondary antibody with a maximum absorption wavelength of 650nm and a maximum emission wavelength of 665nm. Ideal for fluorescent cell and tissue imaging. Suitable for IHC, ICC/IF, Flow Cytometry and ELISA.

- Intense fluorescence and high photostability allowing more time for image capture especially when detecting low abundance targets
- Minimal spectral overlap with other Alexa Fluor® dyes makes this ideal for use in multi-color analysis
- Cited in over 90 publications

查看别名

Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma-2 chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G

2 Images

Immunocytochemistry/ Immunofluorescence - Donkey Anti-Goat IgG H&L (Alexa Fluor® 647) (AB150131)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Donkey Anti-Goat IgG H&L (Alexa Fluor® 647) (AB150131)

ICC/IF image of ab7291 stained HeLa cells. The cells were 100% methanol fixed (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then incubated in 1%BSA / 0.3M glycine in 0.1% PBS-Tween for 1h to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7291, 1μg/ml) overnight at +4°C. ab98800, goat anti-mouse IgG, was then added as a secondary bridging antibody, at 1/250 dilution for 1h. ab150131 Alexa Fluor® 647 donkey anti-goat IgG (H+L) (shown in red) was then used at 1μg/ml for 1h as a tertiary antibody. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.

Alexa Fluor® - Donkey Anti-Goat IgG H&L (Alexa Fluor® 647) (AB150131)

Alexa Fluor®

Unknown

Alexa Fluor® - Donkey Anti-Goat IgG H&L (Alexa Fluor® 647) (AB150131)

关键信息

宿主种属

Donkey

靶标种属

Goat

靶标亚型

IgG

靶标特异性

Heavy & Light chains

最低交叉反应

预吸附

偶联物

Alexa Fluor® 647

激发波长/发射波长

Ex: 650nm, Em: 665nm

应用

ICC/IF, IHC-P, Flow Cyt, ELISA, IHC-Fr

applications

克隆

Polyclonal

亚型

IgG

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/2000", "notes":"" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/200 - 1/1000", "notes":"" } } }

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产品详情

This antibody reacts with the heavy and light chains of Goat IgG

Fluorochrome chart- a complete quick and easy guide to help you select the most appropriate fluorochromes for your next experiment.

When it comes to advancing your immunohistochemistry (IHC) research, Abcam offers comprehensive suite of IHC kits and secondary antibodies, designed to deliver precise and reliable results.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Immunogen

纯化说明

This antibody was isolated by affinity chromatography using antigen coupled to agarose beads.

存储溶液

Preservative: 0.02% Sodium azide Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark

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产品实验方案

Visit the General protocols
Visit the Troubleshooting

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靶点信息

See full target information Goat IgG

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文献 (95)

Recent publications for all applications. Explore the full list and refine your search

Heliyon 9:e22614 PubMed38107325

2023

Decoy peptides effectively inhibit the binding of SARS-CoV-2 to ACE2 on oral epithelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Lai-Keng Loi,Cheng-Chieh Yang,Yu-Cheng Lin,Yee-Fun Su,Yi-Chen Juan,Yi-Hsin Chen,Hsiu-Chuan Chang

Frontiers in nanotechnology 5:1220514 PubMed37954478

2023

Epidermal growth factor alters silica nanoparticle uptake and improves gold-nanoparticle-mediated gene silencing in A549 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Eva Susnik,Amelie Bazzoni,Patricia Taladriz-Blanco,Sandor Balog,Aura Maria Moreno-Echeverri,Christina Glaubitz,Beatriz Brito Oliveira,Daniela Ferreira,Pedro Viana Baptista,Alke Petri-Fink,Barbara Rothen-Rutishauser

Journal of neuroinflammation 20:240 PubMed37864249

2023

Intermittent hypoxia therapy ameliorates beta-amyloid pathology via TFEB-mediated autophagy in murine Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Xueting Wang,Yuqi Xie,Guijuan Chen,Yapeng Lu,Dan Wang,Li Zhu

Scientific reports 13:17874 PubMed37857701

2023

Neuroprotective potential of intranasally delivered L-myc immortalized human neural stem cells in female rats after a controlled cortical impact injury.

Applications

Unspecified application

Species

Unspecified reactive species

Mari Amirbekyan,Vikram Adhikarla,Jeffrey P Cheng,Eleni H Moschonas,Corina O Bondi,Russell C Rockne,Anthony E Kline,Margarita Gutova

JCI insight 8: PubMed37811653

2023

RhoA vesicle trafficking-mediated transglutaminase 2 membrane translocation promotes IgA1 mesangial deposition in IgA nephropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Zhong Zhong,Zhijian Li,Yanjie Li,Lanping Jiang,Qingyu Kong,Wei Chen,Shaozhen Feng

Signal transduction and targeted therapy 8:342 PubMed37691058

2023

Temporospatial inhibition of Erk signaling is required for lymphatic valve formation.

Applications

Unspecified application

Species

Unspecified reactive species

Yaping Meng,Tong Lv,Junfeng Zhang,Weimin Shen,Lifang Li,Yaqi Li,Xin Liu,Xing Lei,Xuguang Lin,Hanfang Xu,Anming Meng,Shunji Jia

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 10:e2303291 PubMed37553778

2023

Lepr-Expressing PDLSCs Contribute to Periodontal Homeostasis and Respond to Mechanical Force by Piezo1.

Applications

Unspecified application

Species

Unspecified reactive species

Danting Zhang,Weimin Lin,Shuang Jiang,Peng Deng,Linfeng Liu,Qian Wang,Rui Sheng,Hui Sophie Shu,Lijun Wang,Weiguo Zou,Bo O Zhou,Junjun Jing,Ling Ye,Bo Yu,Shiwen Zhang,Quan Yuan

Biology 12: PubMed37508377

2023

Corin Deficiency Diminishes Intestinal Sodium Excretion in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Xiabing Gu,Kun Wang,Wenguo Li,Meiling He,Tiantian Zhou,Meng Liu,Qingyu Wu,Ningzheng Dong

Science advances 9:eadf9790 PubMed37327343

2023

Cell competition for neuron-derived trophic factor controls the turnover and lifespan of microglia.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Yu,Haoyue Kuang,Xiaohai Wu,Ying Huang,Jianzhong Wang,Zilong Wen

Nature communications 14:3074 PubMed37244912

2023

Single-cell transcriptomics reveals immune suppression and cell states predictive of patient outcomes in rhabdomyosarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jeff DeMartino,Michael T Meister,Lindy L Visser,Mariël Brok,Marian J A Groot Koerkamp,Amber K L Wezenaar,Laura S Hiemcke-Jiwa,Terezinha de Souza,Johannes H M Merks,Anne C Rios,Frank C P Holstege,Thanasis Margaritis,Jarno Drost

View all publications

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Donkey Anti-Goat IgG H&L (Alexa Fluor® 647)