AB7007

Donkey F(ab')2 Anti-Rabbit IgG H&L (PE) preadsorbed

Name: 驴F(ab')2抗兔IgG H&L (PE)预吸附二抗 (ab7007)| Abcam中文官网
Brand: Abcam Limited
SKU: AB7007
Price: 5457 CNY
Availability: InStock

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(32 Publications)

Suitable for IM, ICC/IF, IHC-P, Flow Cyt, WB. Preadsorbed to minimise non-specific binding and high background staining. Cited in 32 publications.

查看别名

Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma chain C region, Ig gamma-2 chain C region, Ig kappa-b4 chain C region, Ig kappa-b5 chain C region, Ig kappa-b9 chain C region, Ig lambda chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G, K-BAS

2 Images

Flow Cytometry - Donkey F(ab')2 Anti-Rabbit IgG H&L (PE) preadsorbed (AB7007)

Flow Cyt

Unknown

Flow Cytometry - Donkey F(ab')2 Anti-Rabbit IgG H&L (PE) preadsorbed (AB7007)

ab7007 was used with the primary antibody ab18203 in Flow Cyt. See Abreview on ab18203.

Supplier Data

Western blot - Donkey F(ab')2 Anti-Rabbit IgG H&L (PE) preadsorbed (AB7007)

Western blot analysis of ab7007 used as a secondary antibody at 1/1000 dilution for 60 min at RT.

All lanes:

Rabbit IgG at 50 ng

Secondary

All lanes:

Western blot - Donkey F(ab')2 Anti-Rabbit IgG H&L (PE) preadsorbed (ab7007) at 1/1000 dilution

false

关键信息

宿主种属

Donkey

靶标种属

Rabbit

靶标亚型

IgG

靶标特异性

Heavy & Light chains

最低交叉反应

Mouse, Rat, Sheep, Goat, Horse, Chicken, Guinea pig, Hamster, Cow, Human

预吸附

Yes

偶联物

激发波长/发射波长

Ex: 480;565nm, Em: 578nm

应用

Flow Cyt, ICC/IF, IM, IHC-P, WB

applications

克隆

Polyclonal

亚型

IgG

特异性

No reaction was observed against anti-Pepsin, anti-Donkey IgG F(c) or to Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat & Sheep Serum Proteins.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "IM": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/100 - 1/250", "notes":"" }, "IHC-P": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/200", "notes":"" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/100 - 1/250", "notes":"The maximum amount of reagent required to stain 1 x 106 cells in flow cytometry is approximately 1.0 ug of antibody conjugate." }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/1000", "notes":"" } } }

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产品详情

Can be used in immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification

纯化说明

F(ab')2 Anti-Rabbit IgG (H&L) Phycoerythrin Conjugated Antibody Pre-adsorbed was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.

存储溶液

Preservative: 0.01% Sodium azide Constituents: 1% BSA, 0.88% Sodium chloride, 0.424% Potassium phosphate solution

运输条件

Blue Ice

产品实验方案

Visit the General protocols
Visit the Troubleshooting

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靶点信息

See full target information Rabbit IgG

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文献 (32)

Recent publications for all applications. Explore the full list and refine your search

Cells 12: PubMed36611982

2023

Maternal Hyperhomocysteinemia Disturbs the Mechanisms of Embryonic Brain Development and Its Maturation in Early Postnatal Ontogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Dmitrii S Vasilev,Anastasiia D Shcherbitskaia,Natalia L Tumanova,Anastasiia V Mikhel,Yulia P Milyutina,Anna A Kovalenko,Nadezhda M Dubrovskaya,Daria B Inozemtseva,Irina V Zalozniaia,Alexander V Arutjunyan

Basic and clinical neuroscience 13:625-636 PubMed37313021

2022

Transdifferentiation of Human Umbilical Cord-Derived Mesenchymal Stem Cells in Dopaminergic Neurons in a Three-Dimensional Culture.

Applications

Unspecified application

Species

Unspecified reactive species

Ardeshir Moayeri,Rafieh Alizadeh,Hatef Ghasemi Hamidabadi,Maryam Nazm Bojnordi,Ali Niapour,Azim Hedayatpour,Marzieh Darvishi,Fatemeh Heidari,Maryam Soleimani,Leila Elyasi

Frontiers in neuroscience 16:867120 PubMed35495064

2022

Maternal Hypoxia Increases the Excitability of Neurons in the Entorhinal Cortex and Dorsal Hippocampus of Rat Offspring.

Applications

Unspecified application

Species

Unspecified reactive species

Dmitry V Amakhin,Elena B Soboleva,Tatiana Yu Postnikova,Natalia L Tumanova,Nadezhda M Dubrovskaya,Daria S Kalinina,Dmitrii S Vasilev,Aleksey V Zaitsev

Oncogene 40:4884-4893 PubMed34163029

2021

SOX9 is a critical regulator of TSPAN8-mediated metastasis in pancreatic cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Junjian Li,Xiaoliang Chen,Liqun Zhu,Zhenghong Lao,Tianhao Zhou,Lijuan Zang,Weiyu Ge,Mengyi Jiang,Jingxuan Xu,Yuan Cao,Shaoqian Du,Yue Yu,Guangjian Fan,Hongxia Wang

Cells 10: PubMed34207057

2021

Prenatal Hyperhomocysteinemia Induces Glial Activation and Alters Neuroinflammatory Marker Expression in Infant Rat Hippocampus.

Applications

Unspecified application

Species

Unspecified reactive species

Anastasiia D Shcherbitskaia,Dmitrii S Vasilev,Yulia P Milyutina,Natalia L Tumanova,Anastasiia V Mikhel,Irina V Zalozniaia,Alexander V Arutjunyan

Molecular therapy oncolytics 20:187-198 PubMed33665357

2021

IRGM promotes melanoma cell survival through autophagy and is a promising prognostic biomarker for clinical application.

Applications

Unspecified application

Species

Unspecified reactive species

Linlu Tian,Hongxue Meng,Xiao Dong,Xinlei Li,Zilin Shi,Hulun Li,Lie Zhang,Yue Yang,Ruijie Liu,Chunying Pei,Bo Li,Hongwei Xu,Rui Li

Prostaglandins, leukotrienes, and essential fatty 163:102210 PubMed33242781

2020

Prostaglandin F2α in vitro can affect basic inflammatory parameters of mesenchymal stem cells and slight modulating some of their immunomodulatory properties.

Applications

Unspecified application

Species

Unspecified reactive species

Andressa Cristina Antunes Santos,Talita Sartori,Primavera Borelli,Ricardo Ambrosio Fock

Pharmaceuticals (Basel, Switzerland) 13: PubMed33113868

2020

Anakinra Reduces Epileptogenesis, Provides Neuroprotection, and Attenuates Behavioral Impairments in Rats in the Lithium-Pilocarpine Model of Epilepsy.

Applications

Unspecified application

Species

Unspecified reactive species

Alexandra V Dyomina,Olga E Zubareva,Ilya V Smolensky,Dmitry S Vasilev,Maria V Zakharova,Anna A Kovalenko,Alexander P Schwarz,Alexander M Ischenko,Aleksey V Zaitsev

PloS one 15:e0239292 PubMed33002044

2020

Silencing miR-202-3p increases MMP-1 and promotes a brain invasive phenotype in metastatic breast cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Rania Harati,Shirin Hafezi,Aloïse Mabondzo,Abdelaziz Tlili

Pharmaceuticals (Basel, Switzerland) 13: PubMed32645833

2020

Loss of miR-101-3p Promotes Transmigration of Metastatic Breast Cancer Cells through the Brain Endothelium by Inducing COX-2/MMP1 Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Rania Harati,Mohammad G Mohammad,Abdelaziz Tlili,Raafat A El-Awady,Rifat Hamoudi

View all publications

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Donkey F(ab')2 Anti-Rabbit IgG H&L (PE) preadsorbed