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驴抗鸡IgY H&L (FITC) (ab63507)

Reviews (2)Q&A (1)References (18)
Immunohistochemistry (Frozen sections) - Donkey Anti-Chicken IgY H&L (FITC) (ab63507)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Donkey Anti-Chicken IgY H&L (FITC) (ab63507)

Key features and details

  • Donkey Anti-Chicken IgY H&L (FITC)
  • Conjugation: FITC. Ex: 493nm, Em: 528nm
  • Host species: Donkey
  • Isotype: IgG
  • Suitable for: IHC-Fr

概述

  • 产品名称

    驴抗鸡IgY H&L (FITC)
    参阅全部 IgY 二抗

  • 宿主

    Donkey

  • 靶标种属

    Chicken

  • 经测试应用

    适用于: IHC-Frmore details

  • 免疫原

    Purified chicken IgY isolated from normal chicken egg yolks.

  • 偶联物

    FITC. Ex: 493nm, Em: 528nm

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C.

  • 存储溶液

    pH: 7.20
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: PBS, 50% Glycerol (glycerin, glycerine)
  • Concentration information loading...

  • 纯度

    Protein L purified

  • 克隆

    多克隆

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab63507于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-Fr (1)
Use at an assay dependent dilution.
说明
IHC-Fr
Use at an assay dependent dilution.

图片

  • Immunohistochemistry (Frozen sections) - Donkey Anti-Chicken IgY H&L (FITC) (ab63507)
    Immunohistochemistry (Frozen sections) - Donkey Anti-Chicken IgY H&L (FITC) (ab63507)This image is courtesy of an anonymous Abreview.

    ab13970 staining GFP in E10.5 mouse embryo sections by Immunohistochemistry (IHC-Fr - frozen sections).

    Tissue was fixed with paraformaldehyde, permeabilized with PBS/0.5% Triton-X100 and blocked with protein block for 30 minutes at 25°C. Samples were incubated with primary antibody (1/500) for 3 hours at 25°C. FITC-conjugated donkey anti-chicken IgY H&L (ab63507) (1/500) was used as the secondary antibody.

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Donkey Anti-Chicken IgY H&L (FITC) (ab63507)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Donkey Anti-Chicken IgY H&L (FITC) (ab63507)

    ab63507 was used at dilution 1/100 with the primary antibody ab13970 in IHC - Free Floating. See the review on ab13970.

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (18)

发表研究结果有使用 ab63507？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab63507 被引用在 18 文献中.

  • Raut NG  et al. Schwann cells modulate nociception in neurofibromatosis 1. JCI Insight 9:N/A (2024). PubMed: 38258905
  • Hu SW  et al. Engineering of the AAV-Compatible Hair Cell-Specific Small-Size Myo15 Promoter for Gene Therapy in the Inner Ear. Research (Wash D C) 7:0341 (2024). PubMed: 38665848
  • Wang L  et al. Neonatal IL-4 Over-Exposure is Accompanied by Macrophage Accumulation in Dura Mater After Instant Anti-inflammatory Cytokine Response in CSF. Cell Mol Neurobiol 44:18 (2024). PubMed: 38315435
  • Decourtye-Espiard L  et al. Stimulation of GHRH Neuron Axon Growth by Leptin and Impact of Nutrition during Suckling in Mice. Nutrients 15:N/A (2023). PubMed: 36904077
  • Fang A  et al. Porous microneedle patch with sustained delivery of extracellular vesicles mitigates severe spinal cord injury. Nat Commun 14:4011 (2023). PubMed: 37419902
View all Publications for this product

客户评价及客户问答

Show All 评价 Q&A
提交评价 提交问题

1-3 of 3 Abreviews or Q&A

Immunohistochemistry (Frozen sections) abreview using Donkey anti-Chicken (FITC) secondary antibody.

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Cryostat sections of E10.5 mouse embryo, which were fixed with PFA before gelatin/sucrose embedding.
Antigen retrieval is optional in this staining.
Primary antibody: Anti-GFP antibody (ab13970) 1:800 at 4 degree overnight.
Secondary antibody: Donkey Anti-Chicken (FITC) ab63507 at RT 4h (long incubation time of secondary would help to increase the signal).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Feb 25 2014

Immunohistochemistry - Free Floating Abreview for Donkey anti-Chicken IgY H&L (FITC) secondary antibody

 Below Average
Abreviews
abreview image
Application
Immunohistochemistry free floating

Sample: Mouse Tissue sections (Brain and spinal cord)
Specification: Brain and spinal cord

Other product details
Dilution: 1/500
Incubation time: 12 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: PBS

Secondary antibody
Secondary antibody: None used

Additional data
Additional Notes: Please note that the below average score represents a score for an application that was not under the tested applications list on the information sheet.
Abcam response: Thank you for submitting feedback on this antibody in an untested application.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

提交于 Nov 06 2009

Question

Customer inquired about detecting presence of 7 different proteins in brain tissue via IHC-F. Would like 3-4 different host species for primary, secondary antibodies that do not overlap in spectra. Customer is also not sure if Caspase 3, Nogo, and neurofilament are necessary for detecting apoptosis / neuronal response.

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Answer

Thank you for contacting Abcam.

I managed to find antibodies against rat GFAP, FOX3, Iba1, and CD68 all raised in different hosts (chicken, rabbit, goat, and mouse, respectively). They each are highly rated by other researchers and have numerous specific published references. The list for these 4 antibodies is given below:
1. GFAP ab4674 (https://www.abcam.com/gfap-antibody-astrocyte-marker-ab4674.html)
2. FOX3 ab104225 (https://www.abcam.com/fox3neun-antibody-ab104225.html)
3. Iba1 ab5076 (https://www.abcam.com/iba1-antibody-ab5076.html)
4. CD68 ab31630 (https://www.abcam.com/cd68-antibody-ed1-ab31630.html)

I also compiled anti-chicken, rabbit, goat, and mouse secondaries with separate emission spectra. We don't have secondaries for these species that weren't raised in any of the 4 species conjugated to a fluorescent protein with a similar spectrum to DAPI. Thus, I think your best bet is to obtain a far-red filter (Cy5), that way you could still stain with DAPI as well. The donkey secondaries that I found with separate emission spectra are listed below:
1. anti-chicken: FITC (https://www.abcam.com/chicken-igy-secondary-antibody-ab63507.html)
2. anti-rabbit: DyLight 594 (Similar spectrum to Texas Red) (https://www.abcam.com/donkey-polyclonal-secondary-antibody-to-rabbit-igg-h-l-dylight-594-pre-adsorbed-ab96921.html)
3. anti-goat: DyLight 650 (Similar spectrum to Cy5) (https://www.abcam.com/donkey-polyclonal-secondary-antibody-to-goat-igg-h-l-dylight-650-pre-adsorbed-ab96938.html)
4. anti-mouse: DyLight 550 (similar spectrum to TRITC) (https://www.abcam.com/donkey-polyclonal-secondary-antibody-to-mouse-igg-h-l-dylight-550-ab96876.html)

The other antibodies you chose (Nogo, heavy neurofilament, caspase 3) seem to be good choices as well as they are tested in formaldehyde fixed, frozen sections via IHC in rat. Below are the antibodies we discussed against those three proteins:
1. anti-Nogo (https://www.abcam.com/nogo-antibody-ab32298.html)
2. anti-heavy neurofilament (https://www.abcam.com/200-kd-neurofilament-heavy-antibody-ab134459.html)
3. anti-caspase 3 (https://www.abcam.com/active-caspase-3-antibody-ab13847.html)

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

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