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鸡抗小鼠IgG H&L (FITC) (ab6810)

Submit a review Q&A (1)References (2)
Flow Cytometry - Chicken Anti-Mouse IgG H&L (FITC) (ab6810)

    Key features and details

    • Chicken Anti-Mouse IgG H&L (FITC)
    • Conjugation: FITC. Ex: 493nm, Em: 528nm
    • Host species: Chicken
    • Suitable for: WB, Immunomicroscopy, Flow Cyt, ICC/IF, IHC-P, IHC-Fr, ELISA

    Conjugates logo Related conjugates and formulations

    Biotin HRP Texas Red ® TRITC Unconjugated

    概述

    • 产品名称

      鸡抗小鼠IgG H&L (FITC)
      参阅全部 IgG 二抗

    • 宿主

      Chicken

    • 靶标种属

      Mouse

    • 经测试应用

      适用于: WB, Immunomicroscopy, Flow Cyt, ICC/IF, IHC-P, IHC-Fr, ELISAmore details

    • 免疫原

      mouse whole molecule

    • 偶联物

      FITC. Ex: 493nm, Em: 528nm

    性能

    • 形式

      Liquid

    • 存放说明

      Shipped at 4°C. Store at +4°C.

    • 存储溶液

      pH: 6.50
      Preservative: 0.01% Sodium azide
      Constituents: 0.88% Sodium chloride, 1% BSA, 0.424% Tripotassium orthophosphate
    • Concentration information loading...

    • 纯度

      Immunogen affinity purified

    • 纯化说明

      This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

    • 共轭说明

      Diaminotriazinylaminofluorescein (DTAF) (Molecular Weight 530 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 2.3 moles DTAF per mole of Rabbit IgG

    • 克隆

      多克隆

    • 研究领域

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab6810于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    WB
    Use at an assay dependent concentration. Predicted molecular weight: 36 kDa.
    Immunomicroscopy
    Use at an assay dependent concentration.
    Flow Cyt
    Use at an assay dependent concentration.
    ICC/IF
    1/1000 - 1/5000.
    IHC-P
    Use at an assay dependent concentration.
    IHC-Fr
    Use at an assay dependent concentration.
    ELISA
    1/10000 - 1/50000.
    说明
    WB
    Use at an assay dependent concentration. Predicted molecular weight: 36 kDa.
    Immunomicroscopy
    Use at an assay dependent concentration.
    Flow Cyt
    Use at an assay dependent concentration.
    ICC/IF
    1/1000 - 1/5000.
    IHC-P
    Use at an assay dependent concentration.
    IHC-Fr
    Use at an assay dependent concentration.
    ELISA
    1/10000 - 1/50000.

    图片

    • Flow Cytometry - Chicken Anti-Mouse IgG H&L (FITC) (ab6810)
      Flow Cytometry - Chicken Anti-Mouse IgG H&L (FITC) (ab6810)
      Ab6810 was used with the primary antibody ab2213 in Flow Cyt. See Abreview on ab2213.

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (2)

    发表研究结果有使用 ab6810？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab6810 被引用在 2 文献中.

    • Arutyunyan I  et al. Role of VEGF-A in angiogenesis promoted by umbilical cord-derived mesenchymal stromal/stem cells: in vitro study. Stem Cell Res Ther 7:46 (2016). IF . PubMed: 27001300
    • Balaji Raghavendran HR  et al. A comparative study on in vitro osteogenic priming potential of electron spun scaffold PLLA/HA/Col, PLLA/HA, and PLLA/Col for tissue engineering application. PLoS One 9:e104389 (2014). ICC/IF . PubMed: 25140798

    客户评价及客户问答

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    Question

    Hello, I know it says purified IgG, but has it been affinity purified so that it does not cross react with other species. The antibody will be mixed with donkey, goat and other secondary antibodies.

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    Answer

    All the information available for this product is stated on the datasheet. The datasheet reads "This product was prepared from monospecific antiserum by immunoaffinity chromatography using mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities". This antibody is therefore specific for mouse immunoglobulins.

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