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AB325292

Alpaca monoclonal [10E10] Anti-Rabbit IgG antibody (AZdye 568)

Alpaca monoclonal [10E10] Anti-Rabbit IgG antibody (AZdye 568)

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Alpaca monoclonal [10E10] Anti-Rabbit IgG antibody (AZdye 568) (ab325292) is a ready-to-go fluorescent camelid single-domain antibody (sdAb,nanobody, VHH) conjugate with maximum absorption wavelength of 578 nm and a maximum emission wavelength of 602 nm. Ideal for fluorescent cell and tissue imaging. Suitable for ICC/IF applications.

  • Quantitative and reliable readout in fluorescence-based analyses
  • Defined number of fluorophores enhance brightness of signals
  • Improved localization accuracy ideal for super resolution imaging
  • Designed to recognize conventional immunoglobulins with high isotype specificity

查看别名

Ig gamma chain C region

1 Images
Immunocytochemistry/ Immunofluorescence - Alpaca monoclonal [10E10] Anti-Rabbit IgG antibody (AZdye 568) (AB325292)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Alpaca monoclonal [10E10] Anti-Rabbit IgG antibody (AZdye 568) (AB325292)

Immunofluorescent analysis of 4% paraformaldehyde fixed, 0.1% TritonX-100 permeabilized, quenched with 0.1M ammonium chloride, and blocked with 10% normal goat serum in PBS HeLa (human cervical cancer cells) cells labelling Ki67 with ab92742 at 1/1000 (0.85 µg/ml) dilution, followed by ab325292 Alpaca monoclonal [10E10] Anti-Rabbit IgG antibody (AZdye 568) at 1/500 (0.15 µg/ml per sdAb) dilution (red).

Confocal image showing cytoplasmic staining in HeLa cells (shown in red). The counterstain was observed in purple. Nuclear DNA was labelled with DAPI (shown in blue).

Image was taken with a fluorescence microscope (Zeiss Axio Imager Z1).

ab316214 anti-Vimentin antibody was used to counterstain Vimentin at 1/1000 (0.80 µg/ml) dilution (purple).

Secondary antibody only control : Secondary antibody is ab325292 Alpaca monoclonal [10E10] Anti-Rabbit IgG antibody (AZdye 568) at 1/500 (0.15 µg/ml per sdAb) dilution.

关键信息

宿主种属

Alpaca

靶标种属

Rabbit

靶标亚型

IgG

靶标特异性

Fc region

最低交叉反应
预吸附

No

偶联物

AZdye 568

激发波长/发射波长

Ex: 578nm, Em: 602nm

应用

ICC/IF

applications

克隆

Monoclonal

克隆号

10E10

亚型

IgG

免疫原

The exact immunogen used to generate this antibody is proprietary information.

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/500", "notes":"<p></p>" } } }

产品详情

Together with their monovalent binding mode, the defined fluorophore conjugation of ab325292 results in a quantitative and reliable readout for several fluorescence-based applications.  It contains a defined number of fluorophores at known positions. In addition due to the small size the fluorophore displacement from the bound target protein is very low making ab325292 ideal for standard super resolution technologies.

Features:
- High affinity monovalent binding + two fluorophores per sdAb for brighter signals.
- Reagent is made from small-sized proteins. This ensures optimal access to the respective target protein, also in crowded environment or 3D samples.
In comparison to conventional secondary reagents the ab325292 nanobody has the following advantages:
- Small probes for enhanced staining speed and improved tissue penetration and higher resolution.
- Monoclonal tools with defined species  and isotype specificity.
- Recombinant production for reliable quality, scalability and reproducibility.
- Monovalent binding mode – Pre-incubation without clustering primary antibodies and therefore one-step indirect immunofluorescence possible.
- Enhanced binding allows one-step multi-colour staining and imaging even with primary antibodies from identical species and isotype (Multiplexing).
- Suitable for post-fixation and enhanced performance in demanding application (e.g., expansion microscopy, DNA-PAINT, dSTORM, etc.).

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

性能和储存信息

形式
Liquid
纯化工艺
Size-exclusion chromatography
存储溶液
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
运输条件
Blue Ice
推荐的短期储存时间
1 month
推荐的短期储存条件
-20°C
推荐的长期储存条件
-80°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle

产品实验方案

websiteProtocolBooklet
en
websiteProtocolBooklet
en

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