蛋白A Magnetic Beads (ab214286)
Key features and details
- Sample type: Cell Lysate, Serum
概述
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产品名称
蛋白A Magnetic Beads -
样品类型
Serum, Cell Lysate -
种属反应性
与反应: Mouse, Rabbit, Horse, Guinea pig, Hamster, Cow, Human, Pig
不与反应: Rat, Sheep, Goat, Chicken -
产品概述
Features:
Easy to use, high-binding capacity, non-adherent beads.
Support Characteristics: Paramagnetic, spherical, 6 % cross-linked agarose.
Ligand: Recombinant Protein A.
Particle Size: 75 – 150 µm.
Binding Capacity: Generally >25 mg human IgG/ml wet beads.
Working Temperature: Room temperature.
Storage Solution: PBS with 0.02% Sodium Azide.
Storage Temperature: 4 – 8 °C.
Applications:
Useful for immunoprecipitation and enrichment of IgG antibodies.
High affinity for Fc region of IgG antibodies from a variety of species.
Protein A binds to most human and mouse IgG subclasses (e.g., human IgG1, IgG2, IgG4; mouse IgG1, IgG2a, IgG2b, IgG3).
It also binds to total IgG from cow, guinea pig, hamster, horse, pig, and rabbit. Protein A has little affinity to chicken, goat, rat and sheep.
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说明
This product is manufactured by BioVision, an Abcam company and was previously called 6507 Protein A Magnetic Beads. 6507-1 is the same size as the 1 ml size of ab214286.
Description:
Protein A Magnetic Beads are prepared by covalently coupling Recombinant Protein A to 6% crosslinked magnetically beaded agarose. The coupling technique is optimized to give a high binding capacity for IgG. The capacity of IgG binding is generally greater than 25 mg of human IgG per ml of wet gel.
SUGGESTED PROTOCOL:
Prepare the antibody solution by diluting the required amount of antibody in binding buffer before running the protocol.
1. Magnetic Bead Preparation (perform three times)
a. Dispense the required amount of magnetic beads into a 1.5 ml microfuge tube.
b. Place the tube in the magnetic rack and remove the storage solution.
c. Add 500 µl binding buffer.
d. Resuspend the beads.
e. Remove the liquid
2. Antibody Capture
a. Immediately add the antibody solution.
b. Resuspend and mix (slow end-over-end) for at least 15 minutes.
c. Remove the liquid.
3. Washing
a. Add 500 µl Binding Buffer containing 0.5 M NaCl; Remove the liquid.
b. Add 500 µl Binding Buffer; Remove the liquid.
4. Target Binding
a. Add sample diluted in binding buffer.
b. Incubate with slow end-over-end mixing for up to 60 minutes.
c. Remove and collect unbound fraction.
5. Washing ( perform three times)
a. Add 500 µl wash buffer
b. Remove liquid (save washes to troubleshoot)
6. Elution (perform three times)
a. Add 2 volumes elution buffer (vs. bead volume).
b. Completely resuspend beads and incubate at least 2 minutes.
c. Remove and collect elution fraction.
RECOMMENDED BUFFER EXAMPLES:
Binding buffer: 50 mM Tris, 150 mM NaCl, pH 7.5
Wash buffer: 50 mM Tris, 150 mM NaCl, pH 7.5 (or add 1% Octylglucoside to this buffer) (Could also try 1X PBS as both binding and wash buffer)
Elution buffer: 0.1 M -0.2 M Glycine pH 2.5-3.1 (or 0.1 M citric acid, pH 2.5-3.1 or 2.5 % Acetic Acid)
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经测试应用
适用于: Purification, IPmore details
性能
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存放说明
Store at +4°C. Please refer to protocols. -
组件 1 ml Protein A magnetic beads 1 x 1ml
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab214286于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Purification |
Use at an assay dependent concentration.
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IP |
Use at an assay dependent concentration.
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说明 |
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Purification
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab214286 被引用在 5 文献中.
- Chen X et al. Targeting the CtBP1-FOXM1 transcriptional complex with small molecules to overcome MDR1-mediated chemoresistance in osteosarcoma cancer stem cells. J Cancer 12:482-497 (2021). PubMed: 33391445
- Samuels TJ et al. Neuronal upregulation of Prospero protein is driven by alternative mRNA polyadenylation and Syncrip-mediated mRNA stabilisation. Biol Open 9:N/A (2020). PubMed: 32205310
- Xu J et al. Globular C1q Receptor (gC1qR/p32/HABP1) Suppresses the Tumor-Inhibiting Role of C1q and Promotes Tumor Proliferation in 1q21-Amplified Multiple Myeloma. Front Immunol 11:1292 (2020). PubMed: 32760394
- Wei L et al. Novel urokinase-plasminogen activator inhibitor SPINK13 inhibits growth and metastasis of hepatocellular carcinoma in vivo. Pharmacol Res 143:73-85 (2019). PubMed: 30862605
- Meng D et al. MicroRNA-645 targets urokinase plasminogen activator and decreases the invasive growth of MDA-MB-231 triple-negative breast cancer cells. Onco Targets Ther 11:7733-7743 (2018). PubMed: 30464522