AB289882

核/Cytosol Fractionation试剂盒

Name: Nuclear/Cytosol Fractionation试剂盒 (ab289882)| Abcam中文官网
Brand: Abcam Limited
SKU: AB289882
Price: 5667 CNY
Availability: InStock

Nuclear/Cytosol Fractionation Kit

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(16 Publications)

Nuclear/Cytosol Fractionation Kit ab289882 for isolation of nuclear/cytosol fractions without ultracentrifugation

- Simple procedure; 2-3 hours
- little or no cross-contamination

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs

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Other - Nuclear/Cytosol Fractionation Kit (AB289882)

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Supplier Data

Other - Nuclear/Cytosol Fractionation Kit (AB289882)

Representative image of Nuclear/Cytosol Fractionation Kit ab289882

Components shown from left to right :

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit.

关键信息

样品类型

Tissue, Suspension cells, Adherent cells

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产品详情

Nuclear/Cytosol Fractionation Kit ab289882 is a complete kit that allows isolation of nuclear/cytosol fractions without ultracentrifugation.

The optimized reagents and procedures provided with the kit allow separation of nuclear and cytoplasmic fractions quickly with little or no cross-contamination. The extracted nuclear and cytoplasmic protein fractions are functional and compatible with downstream assays such as transcriptional activity, RNA splicing, gel shift assay, reporter assays, enzyme activity assays, and Western blotting.

Nuclear/Cytosol fractionation protocol summary

- Collect cells by centrifugation

-Add Extraction buffer

- Vortex

-Incubate on Ice for 10 min

- Add lysis/cytosol extraction buffer

- Vortex

- Incubate on ice for 1 min

-Vortex then centrifuge

- Transfer the supernatant cytoplasmic extract fraction to a clean prechilled tube and place on ice

- Resuspend pellet (nuclei) and vortex and centrifuge

- Transfer the superanatant (nuclear extract) to a clean pre-chilled tube.

- Place on ice and store extract at -80°C for future use

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

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规格

{ "values": { "100Tests": { "sellingSize": "100 Tests", "publicAssetCode":"ab289882-100Tests", "assetComponentDetails": [ { "size":"1 x 1.2 mL", "name":"Lysis Buffer X", "number":"AB289882-CMP02", "productcode":"" }, { "size":"1 x 10 mL", "name":"Extraction Buffer V", "number":"AB289882-CMP05", "productcode":"" }, { "size":"1 x 100 µL", "name":"DTT II", "number":"AB289882-CMP04", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Protease Inhibitor Cocktail I", "number":"AB289882-CMP03", "productcode":"" }, { "size":"1 x 20 mL", "name":"Extraction Buffer VI", "number":"AB289882-CMP01", "productcode":"" } ] } } }

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性能和储存信息

运输条件

Blue Ice

储存信息

-20°C

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产品实验方案

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靶点信息

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文献 (16)

Recent publications for all applications. Explore the full list and refine your search

The Journal of clinical investigation 135: PubMed40526428

2025

Allergens abrogate antiinflammatory DNA effects and unmask macrophage-driven neutrophilic asthma via ILC2/STING/TNF-α signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Anand Sripada,Divya Verma,Rangati Varma,Kapil Sirohi,Carolyn Kwiat,Mohini Pathria,Mukesh Verma,Anita Sahu,Vamsi Guntur,Laurie Manka,Brian Vestal,Camille Moore,Richard J Martin,Magdalena M Gorska,John Cambier,Andrew Getahun,Rafeul Alam

The Kaohsiung journal of medical sciences 41:e12948 PubMed40035259

2025

LINC01871 facilitates cervical cancer cell migration and immune escape by targeting miR-873-3p/MAP3K2 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Li,Li Wei,Hui-Hui Zhang,Hong-Fei Ci,Duo-Jie Li

American journal of cancer research 15:363-374 PubMed39949934

2025

Overexpression of SHANK2 contributes malignant outcomes as a Hippo pathway regulator in gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Hiroshi Arakawa,Shuhei Komatsu,Jun Kiuchi,Taisuke Imamura,Keiji Nishibeppu,Hajime Kamiya,Yusuke Takashima,Ryo Ishida,Satoshi Hamada,Masateru Yamauchi,Takuma Ohashi,Hiroki Shimizu,Tomohiro Arita,Hirotaka Konishi,Atsushi Shiozaki,Takeshi Kubota,Hitoshi Fujiwara,Hitoshi Tsuda,Eigo Otsuji

Molecular medicine (Cambridge, Mass.) 30:246 PubMed39701936

2024

Depressed TFAM promotes acetaminophen-induced hepatotoxicity regulated by DDX3X-PGC1α-NRF2 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Sisi Chen,Yaling Cao,Zihao Fan,Ling Xu,Zhenzhen Pan,Yao Gao,Linlin Wei,Qiaoxin Wei,Yuan Tian,Xiangying Zhang,Mei Liu,Feng Ren

Environmental toxicology 40:165-178 PubMed39324672

2024

Black Tea Suppresses Invasiveness and Reverses TNF-α-Induced Invasiveness and Cell Stemness in Human Malignant Melanoma Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Chin-Yin Lin,Shu-Chen Chu,Yih-Shou Hsieh,Wen-Yi Tsai,Pei-Ni Chen

Journal of experimental & clinical cancer research : CR 43:227 PubMed39148124

2024

Palmitoyltransferase ZDHHC6 promotes colon tumorigenesis by targeting PPARγ-driven lipid biosynthesis via regulating lipidome metabolic reprogramming.

Applications

Unspecified application

Species

Unspecified reactive species

Junqi Shan,Xinyu Li,Runqi Sun,Yao Yao,Yan Sun,Qin Kuang,Xianling Dai,Yanlai Sun

Oncology reports 51: PubMed38577936

2024

Butyrate increases methylglyoxal production through regulation of the JAK2/Stat3/Nrf2/Glo1 pathway in castration‑resistant prostate cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yi-Jan Hsia,Zhang-Min Lin,Taolan Zhang,Tz-Chong Chou

Respiratory research 25:108 PubMed38419044

2024

FGF18 alleviates sepsis-induced acute lung injury by inhibiting the NF-κB pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenyu Hu,Jindan Dai,Tianpeng Xu,Hui Chen,Guoxiu Shen,Jie Zhou,Hongfang Ma,Yang Wang,Litai Jin

Nature methods 21:584-596 PubMed38409225

2024

Genome-scale pan-cancer interrogation of lncRNA dependencies using CasRx.

Applications

Unspecified application

Species

Unspecified reactive species

Juan J Montero,Riccardo Trozzo,Maya Sugden,Rupert Öllinger,Alexander Belka,Ekaterina Zhigalova,Paul Waetzig,Thomas Engleitner,Marc Schmidt-Supprian,Dieter Saur,Roland Rad

Poultry science 103:103361 PubMed38154448

2023

Wnt signaling blockade is essential for maintaining the pluripotency of chicken embryonic stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ryota Kajihara,Ryo Ezaki,Kennosuke Ichikawa,Tenkai Watanabe,Takumi Terada,Mei Matsuzaki,Hiroyuki Horiuchi

View all publications

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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

核/Cytosol Fractionation试剂盒

Nuclear/Cytosol Fractionation Kit

Other - Nuclear/Cytosol Fractionation Kit (AB289882)

关键信息

样品类型

产品详情