AB110170

Mitochondria分离试剂盒for Cultured Cells

Name: Mitochondria Isolation试剂盒for Cultured Cells (ab110170)| Abcam中文官网
Brand: Abcam Limited
SKU: AB110170
Price: 2752 CNY
Availability: InStock
Rating: 5 (2 reviews)

Mitochondria Isolation Kit for Cultured Cells

(2 Reviews)

(97 Publications)

Mitochondria isolation kit for cultured cells (ab110170) is designed for efficient isolation of mitochondria from cultured mammalian cells using differential centrifugation. Suitable for Western blot, immunocapture or activity assays.

- Provides reagents for up to 20 isolations
- Proven performance: cited in over 75 publications

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Supplier Data

Western blot - Mitochondria Isolation Kit for Cultured Cells (AB110170)

Isolated mitochondria show enriched signal when compared to the whole cell extract. In lane 1, MRC5 mitochondria isolated with Abcam's ab110170 (MS852) Mitochondria Isolation Kit for Cultured Cells were loaded at 20 µg. In lanes 2 and 3, post-spin supernatant (SN) and whole cell extract were loaded at 20 µg.

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关键信息

检测时间

反应种属

Mammals

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产品详情

ab110170 contains sufficient reagents for up to 20 benchtop mitochondria isolations from mammalian cultured cells. Isolated mitochondria can be analyzed by Western Blotting, Immunocapture, or Activity assay.

Available with a dounce homogenizer as kit ab110171.

Mitochondrial isolation protocol summary:
- suspend cells in reagent A and incubate for 10 min
- homogenize using dounce homogenizer
- spin at 1,000 g for 10 min and retain supernatant, repeat
- spin at 12,000 g for 15 min and retain pellet
- resuspend pellet in reagent C and analyze or freeze

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规格

{ "values": { "1Kit": { "sellingSize": "1 Kit", "publicAssetCode":"ab110170-1Kit", "assetComponentDetails": [ { "size":"1 x 10 mL", "name":"Reagent C", "number":"AB110170-CMP03", "productcode":"" }, { "size":"1 x 50 mL", "name":"Reagent B", "number":"AB110170-CMP02", "productcode":"" }, { "size":"1 x 50 mL", "name":"Reagent A", "number":"AB110170-CMP01", "productcode":"" } ] } } }

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性能和储存信息

运输条件

Blue Ice

储存信息

+4°C

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产品实验方案

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靶点信息

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文献 (97)

Recent publications for all applications. Explore the full list and refine your search

Cell death and differentiation : PubMed40404919

2025

NDUFS3 promotes proliferation via glucose metabolism reprogramming inducing AMPK phosphorylating PRPS1 to increase the purine nucleotide synthesis in melanoma.

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Unspecified application

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Unspecified reactive species

Guohang Xiong,Fang Yun,Lu Jiang,Zihan Yi,Xiaojia Yi,Lijuan Yang,Xuedan Zhang,Xiaoyu Li,Zhe Yang,Qiao Zhang,Buqing Sai,Yingmin Kuang,Yuechun Zhu

Cell death & disease 16:253 PubMed40185729

2025

Mitochondrial, metabolic and bioenergetic adaptations drive plasticity of colorectal cancer cells and shape their chemosensitivity.

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Species

Unspecified reactive species

Nikita Markov,Sirina Sabirova,Gulnaz Sharapova,Marina Gomzikova,Anna Brichkina,Nick A Barlev,Marcel Egger,Albert Rizvanov,Hans-Uwe Simon

Cell death & disease 16:173 PubMed40075073

2025

CHCHD2 rescues the mitochondrial dysfunction in iPSC-derived neurons from patient with Mohr-Tranebjaerg syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Yihua Huang,Zirui Chen,Weiling Deng,Yawei Jiang,Yue Pan,Zhirong Yuan,Hailiang Hu,Yongming Wu,Yafang Hu

Stem cell research & therapy 16:129 PubMed40069892

2025

Enhanced mitochondrial function and delivery from adipose-derived stem cell spheres via the EZH2-H3K27me3-PPARγ pathway for advanced therapy.

Applications

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Species

Unspecified reactive species

Ming-Min Chang,Dinh Toi Chu,Sheng-Che Lin,Jung-Shun Lee,Thuy Duong Vu,Hue Thi Vu,Thamil Selvee Ramasamy,Shau-Ping Lin,Chia-Ching Wu

Experimental & molecular medicine 56:2491-2502 PubMed39511428

2024

Lactate utilization in Lace1 knockout mice promotes browning of inguinal white adipose tissue.

Applications

Unspecified application

Species

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Youn Ju Kim,Sang Gyu Lee,Su In Jang,Won Kon Kim,Kyoung-Jin Oh,Kwang-Hee Bae,Hye Jin Kim,Je Kyung Seong

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2404391 PubMed39444078

2024

Membrane Associated RNA-Containing Vesicles Regulate Cortical Astrocytic Microdomain Calcium Transients in Awake Ischemic Stroke Mice.

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Zhongqiu Zhou,Ying Bai,Xiaochun Gu,Hui Ren,Wen Xi,Yu Wang,Liang Bian,Xue Liu,Ling Shen,Xianyuan Xiang,Wenhui Huang,Zhuojuan Luo,Bing Han,Honghong Yao

Journal of biochemical and molecular toxicology 38:e23854 PubMed39287333

2024

Malate dehydrogenase-2 inhibition shields renal tubular epithelial cells from anoxia-reoxygenation injury by reducing reactive oxygen species.

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Georgios Pissas,Maria Tziastoudi,Maria Divani,Christina Poulianiti,Maria Anna Polyzou Konsta,Evangelos Lykotsetas,Vasilios Liakopoulos,Ioannis Stefanidis,Theodoros Eleftheriadis

eLife 13: PubMed39269443

2024

The bacterial quorum sensing signal 2'-aminoacetophenone rewires immune cell bioenergetics through the Ppargc1a/Esrra axis to mediate tolerance to infection.

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Arijit Chakraborty,Arunava Bandyopadhaya,Vijay K Singh,Filip Kovacic,Sujin Cha,William M Oldham,A Aria Tzika,Laurence G Rahme

Antioxidants (Basel, Switzerland) 13: PubMed39199267

2024

A Multi-Target Pharmacological Correction of a Lipoyltransferase Gene Mutation in Patient-Derived Cellular Models.

Applications

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Unspecified reactive species

David Gómez-Fernández,Ana Romero-González,Juan M Suárez-Rivero,Paula Cilleros-Holgado,Mónica Álvarez-Córdoba,Rocío Piñero-Pérez,José Manuel Romero-Domínguez,Diana Reche-López,Alejandra López-Cabrera,Salvador Ibáñez-Mico,Marta Castro de Oliveira,Andrés Rodríguez-Sacristán,Susana González-Granero,José Manuel García-Verdugo,José A Sánchez-Alcázar

Biomolecules 14: PubMed38786005

2024

Polydatin and Nicotinamide Rescue the Cellular Phenotype of Mitochondrial Diseases by Mitochondrial Unfolded Protein Response (mtUPR) Activation.

Applications

Unspecified application

Species

Unspecified reactive species

Paula Cilleros-Holgado,David Gómez-Fernández,Rocío Piñero-Pérez,José Manuel Romero Domínguez,Marta Talaverón-Rey,Diana Reche-López,Juan Miguel Suárez-Rivero,Mónica Álvarez-Córdoba,Ana Romero-González,Alejandra López-Cabrera,Marta Castro De Oliveira,Andrés Rodríguez-Sacristan,José Antonio Sánchez-Alcázar

View all publications

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Mitochondria分离试剂盒for Cultured Cells

Mitochondria Isolation Kit for Cultured Cells

Western blot - Mitochondria Isolation Kit for Cultured Cells (AB110170)

关键信息

检测时间

反应种属

产品详情