HeLa全细胞RIPA Extract (ab170197)
Key features and details
- Sample type: Cell Lysate
概述
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产品名称
HeLa全细胞RIPA Extract -
样品类型
Cell Lysate -
产品概述
HeLa cells are an adherent human epithelial cell line derived from a cervical carcinoma. HeLa cells were lysed using radio immunoprecipitation assay (RIPA) buffer supplemented with protease and phosphatase inhibitors, centrifuged and the supernatant collected. Protein concentration of the extract was determined by BCA assay. This products is suitable for sandwich ELISAs, Immunoprecipitation, SDS-PAGE and Western Blotting.
Avoid freeze-thaw cycles with this product.
Concentration: Each unit contains a minimum of 300 µg at a lot-specific concentration. See label for concentration.
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说明
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
经测试应用
适用于: SDS-PAGE, IP, WB, Blocking, Sandwich ELISAmore details
性能
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存放说明
Store at -80°C. Please refer to protocols. -
组件 2 units HeLa Whole Cell RIPA Extract 2 vials -
研究领域
相关产品
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab170197于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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SDS-PAGE |
Use at an assay dependent concentration.
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IP |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration.
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Blocking |
Use at an assay dependent concentration.
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Sandwich ELISA |
Use at an assay dependent concentration.
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说明 |
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SDS-PAGE
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. |
Blocking
Use at an assay dependent concentration. |
Sandwich ELISA
Use at an assay dependent concentration. |
图片
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Glucose 6 Phosphate Isomerase (G6PI) protein was assayed in HeLa Whole Cell RIPA Extract (ab170197) in triplicate using Glucose 6 Phosphate Isomerase Human ELISA Kit (ab128574). A standard curve generated using the G6PI standard protein is shown (left image). G6PI signal using 2-fold serial dilutions of ab170197 starting at 50 µg/mL (right image). The G6PI concentration in the HeLa Whole Cell RIPA Extract was interpolated to be 24.9 µg/mL.
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Mitochondrial Transcription Factor A (mtTFA) protein was assayed in HeLa Whole Cell RIPA Extract (ab170197) in triplicate using mtTFA (TFAM) Human ELISA Kit (ab123455). A standard curve generated using the mtTFA standard protein is shown (left panel). mtTFA signal using 2-fold serial dilutions of ab170197 starting at 400 µg/mL is shown (right panel). The mtTFA concentration in the HeLa Whole Cell RIPA Extract was interpolated to be 841 ng/mL.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab170197 尚未被引用在任何文献中。