AB170197

HeLa Whole Cell RIPA Extract

Name: HeLa Whole Cell RIPA Extract (ab170197)| Abcam中文官网
Brand: Abcam Limited
SKU: AB170197
Price: 1269 CNY
Availability: InStock

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(0 Publication)

HeLa cells are an adherent human epithelial cell line derived from a cervical carcinoma.

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ELISA - HeLa Whole Cell RIPA Extract (AB170197)

ELISA

Supplier Data

ELISA - HeLa Whole Cell RIPA Extract (AB170197)

Glucose 6 Phosphate Isomerase Human ELISA Kit (ab128574) using HeLa Whole Cell RIPA Extract.

Glucose 6 Phosphate Isomerase (G6PI) protein was assayed in HeLa Whole Cell RIPA Extract (ab170197) in triplicate using Glucose 6 Phosphate Isomerase Human ELISA Kit (ab128574). A standard curve generated using the G6PI standard protein is shown (left image). G6PI signal using 2-fold serial dilutions of ab170197 starting at 50 μg/mL (right image). The G6PI concentration in the HeLa Whole Cell RIPA Extract was interpolated to be 24.9 μg/mL.

ELISA

Supplier Data

ELISA - HeLa Whole Cell RIPA Extract (AB170197)

mtTFA (TFAM) Human ELISA Kit (ab123455) using HeLa Whole Cell RIPA Extract.

Mitochondrial Transcription Factor A (mtTFA) protein was assayed in HeLa Whole Cell RIPA Extract (ab170197) in triplicate using mtTFA (TFAM) Human ELISA Kit (ab123455). A standard curve generated using the mtTFA standard protein is shown (left panel). mtTFA signal using 2-fold serial dilutions of ab170197 starting at 400 μg/mL is shown (right panel). The mtTFA concentration in the HeLa Whole Cell RIPA Extract was interpolated to be 841 ng/mL.

关键信息

样品类型

Cell Lysate

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "BL": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IP": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" } } }

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产品详情

HeLa cells are an adherent human epithelial cell line derived from a cervical carcinoma. HeLa cells were lysed using radio immunoprecipitation assay (RIPA) buffer supplemented with protease and phosphatase inhibitors, centrifuged and the supernatant collected. Protein concentration of the extract was determined by BCA assay. This products is suitable for sandwich ELISAs, Immunoprecipitation, SDS-PAGE and Western Blotting.

Avoid freeze-thaw cycles with this product.

Concentration: Each unit contains a minimum of 300 μg at a lot-specific concentration. See label for concentration.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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规格

{ "values": { "2Unit": { "sellingSize": "2 Unit", "publicAssetCode":"ab170197-2Unit", "assetComponentDetails": [ { "size":"2 x 1 Vial", "name":"HeLa Whole Cell RIPA Extract", "number":"AB170197-CMP01", "productcode":"" } ] } } }

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性能和储存信息

运输条件

Dry Ice

储存信息

-80°C

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产品实验方案

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靶点信息

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HeLa Whole Cell RIPA Extract