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AB204708

Deproteinizing Sample准备试剂盒- TCA

Deproteinizing Sample Preparation Kit - TCA

4

(1 Review)

|

(90 Publications)

Deproteinizing Sample Preparation Kit - TCA ab204708 offers a simple method to prepare samples for use in assays to measure small molecules, such as lipids, metabolites, metal ions etc.

Individual kit components also available for purchase with a minimum order of 20 units. Contact us to discuss your needs.
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Functional Studies - Deproteinizing Sample Preparation Kit - TCA (AB204708)
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Supplier Data

Functional Studies - Deproteinizing Sample Preparation Kit - TCA (AB204708)

ab 204708.

Effect of TCA amount on deproteinization :

Aliquots of pooledhuman serum (off the clot, 100 μL) were treated with different amounts of TCA following the kit protocol. Estimated protein concentration before deproteinization : 7.9 g/dL. More than 99% of protein was removed using less than 10 μL of TCA/100 μL human serum. Red solid line = Total protein; blue dotted line = sample treated with TCA; green solid line = sample treated with spin column.

Functional Studies - Deproteinizing Sample Preparation Kit - TCA (AB204708)
  • FuncS

Supplier Data

Functional Studies - Deproteinizing Sample Preparation Kit - TCA (AB204708)

ab 204708.

Comparison of analysis of lactate :

Human serum samples (100 μL) were analyzed for lactate concentration using L-Lactate Assay Kit (Colorimetric) (ab65331). Untreated samples (purple line) show higher signal when compared to TCA treated samples (red line). However, background signal is very similar in both untreated (green line) and treated (blue line) samples.

关键信息

检测时间

25m

样品类型

Tissue Lysate, Urine, Tissue Extracts, Whole Blood, Tissue Culture Media

产品详情

Deproteinizing Sample Preparation Kit - TCA (ab204708) offers a simple method to prepare samples for use in assays to measure small molecules, such as lipids, metabolites, metal ions etc.

This kit is useful where endogenous enzymes and other proteins would interfere with the assay, or would act directly to affect the level of the analyte to be measured, or would bind the analyte.

In this kit, trichloroacetic acid (TCA) is used to precipitate proteins by drastically lowering the sample pH. After removal of precipitated proteins, the pH of the sample is neutralized with neutralization buffer that is provided in the kit. Small molecules present in the sample can then be easily analyzed without interference from endogenous enzymes and other proteins .

The kit is easy to follow, convenient, and can be utilized for the preparation of a large number of samples in parallel. Samples prepared using this kit can be directly used in various bioassays.

This product is manufactured by BioVision, an Abcam company and was previously called K823 Deproteinizing Sample Preparation Kit II. K823-200 is the same size as the 200 test size of ab204708.

The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.

规格

{ "values": { "200Test": { "sellingSize": "200 Test", "publicAssetCode":"ab204708-200Test", "assetComponentDetails": [ { "size":"1 x 4 mL", "name":"Neutralization Buffer I", "number":"AB204708-CMP01", "productcode":"" }, { "size":"1 x 3 mL", "name":"Trichloroacetic Acid Solution", "number":"AB204708-CMP02", "productcode":"" } ] } } }

性能和储存信息

运输条件
Ambient - Cannot Ship with Ice
推荐的短期储存条件
Ambient
推荐的长期储存条件
Ambient
储存信息
Ambient

产品实验方案

文献 (90)

Recent publications for all applications. Explore the full list and refine your search

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