VeriBlot for IP Detection Reagent (HRP)

• IP

Supplier Data

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

SLAMF6 was immunoprecipitated from 0.35 mg Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate with ab224201 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab224201 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

All lanes:

Immunoprecipitation - Anti-SLAMF6 antibody [EPR22170] (<a href='/products/primary-antibodies/slamf6-antibody-epr22170-ab224201'>ab224201</a>) at 1/500 dilution

Lane 1:

Jurkat whole cell lysate (input) at 10 µg

Lane 2:

<a href='/products/primary-antibodies/slamf6-antibody-epr22170-ab224201'>ab224201</a> at 1/30 IP in Jurkat whole cell lysate (+) at 10 µg

Lane 3:

Rabbit monoclonal IgG (<a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of <a href='/products/primary-antibodies/slamf6-antibody-epr22170-ab224201'>ab224201</a> in Jurkat whole cell lysate (-) at 10 µg

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

Predicted band size: 37 kDa

true

Exposure time: 10s

• IP

Supplier Data

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

E2F2 was immunoprecipitated from 0.35 mg Raji (human Burkitt's lymphoma B lymphocyte) whole cell lysate with ab318956 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab318956 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : Raji (human Burkitt's lymphoma B lymphocyte) whole cell lysate
Lane 2 : ab318956 IP in Raji (human Burkitt's lymphoma B lymphocyte) whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab318956 in Raji whole cell lysate

All lanes:

Immunoprecipitation - Anti-E2F2 antibody [EPR27391-170] (<a href='/products/primary-antibodies/e2f2-antibody-epr27391-170-ab318956'>ab318956</a>) at 1/30 dilution

All lanes:

Raji (human Burkitt's lymphoma B lymphocyte) whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution

false

Exposure time: 180s

• IP

Supplier Data

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

LONP1/Lon was immunoprecipitated from 0.35 mg HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate with ab317561 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab317561 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : ab317561 IP in HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab317561 in HeLa whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Bands observed below the major band at 106kDa in lane2 represent the isoforms of LONP1/Lon.

All lanes:

Immunoprecipitation - Anti-LONP1/Lon antibody [EPR28510-62] (<a href='/products/primary-antibodies/lonp1-lon-antibody-epr28510-62-ab317561'>ab317561</a>) at 1/30 dilution

All lanes:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution

false

Exposure time: 3s

• IP

Supplier Data

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

Histone H3 (di methyl K36) was immunoprecipitated from 0.35 mg HeLa (human cervical adenocarcinoma epithelial cell) histone lysate with ab318964 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab318964 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : HeLa (human cervical adenocarcinoma epithelial cell) histone lysate
Lane 2 : ab318964 IP in HeLa (human cervical adenocarcinoma epithelial cell) histone lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab318964 in HeLa histone lysate

All lanes:

Immunoprecipitation - Anti-Histone H3 (di methyl K36) antibody [EPR28335-73] (<a href='/products/primary-antibodies/histone-h3-di-methyl-k36-antibody-epr28335-73-ab318964'>ab318964</a>) at 1/30 dilution

All lanes:

HeLa (human cervical adenocarcinoma epithelial cell) histone lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution

false

Exposure time: 180s

• IP

Supplier Data

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

E2F2 was immunoprecipitated from 0.35 mg Ramos (human Burkitt's lymphoma B lymphocyte) whole cell lysate with ab318956 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab318956 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : Ramos (human Burkitt's lymphoma B lymphocyte) whole cell lysate
Lane 2 : ab318956 IP in Ramos (human Burkitt's lymphoma B lymphocyte) whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab318956 in Ramos whole cell lysate

All lanes:

Immunoprecipitation - Anti-E2F2 antibody [EPR27391-170] (<a href='/products/primary-antibodies/e2f2-antibody-epr27391-170-ab318956'>ab318956</a>) at 1/30 dilution

All lanes:

Ramos (human Burkitt's lymphoma B lymphocyte) whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution

false

Exposure time: 180s

• IP

Supplier Data

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

RPL11 was immunoprecipitated from 0.35 mg HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate with ab318976 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab318976 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : ab318976 IP in HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab318976 in HeLa whole cell lysate

All lanes:

Immunoprecipitation - Anti-RPL11 antibody [EPR28901-64] (<a href='/products/primary-antibodies/rpl11-antibody-epr28901-64-ab318976'>ab318976</a>) at 1/30 dilution

All lanes:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution

false

Exposure time: 10s

• IP

Supplier Data

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

Cdc20 was immunoprecipitated from 0.35 mg PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate with ab317036 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab317036 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : ab317036 IP in PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab317036 in PC-3 whole cell lysate

The band at ~37KDa may represent a truncated Cdc20 isoform (PMID : 37100900).

All lanes:

Immunoprecipitation - Anti-Cdc20 antibody [EPR28091-55] (<a href='/products/primary-antibodies/cdc20-antibody-epr28091-55-ab317036'>ab317036</a>) at 1/30 dilution

All lanes:

PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution

false

Exposure time: 180s

• IP

Lab

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

BRG1 was immunoprecipitated from K-562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate with ab108318 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab108318 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used as secondary antibody at 1/5000 dilution. Lane 1 (Input) : K-562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate, 10 μg Lane 2 (+) : K-562 whole cell lysate Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab108318 in K-562 whole cell lysate Blocking and diluting buffer and concentration : 5% NFDM/TBST. Observed MW : 185 kDa.

All lanes:

Immunoprecipitation - Anti-BRG1 antibody [EPR3912] (<a href='/products/primary-antibodies/brg1-antibody-epr3912-ab108318'>ab108318</a>) at 1/1000 dilution

Lane 1:

K-562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate at 10 µg

Lane 2:

K-562 whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution

Predicted band size: 185 kDa

Observed band size: 185 kDa

false

• IP

Lab

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

RPL26 was immunoprecipitated from 0.35 mg HEK-293 (human embryonic kidney epithelial cell) whole cell lysate with ab325434 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab325434 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : HEK-293 (human embryonic kidney epithelial cell) whole cell lysate
Lane 2 : ab325434 IP in HEK-293 (human embryonic kidney epithelial cell) whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab325434 in HEK-293 whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 180 seconds.

All lanes:

Immunoprecipitation - Anti-RPL26+RPL26L antibody [EPR28600-70] (<a href='/products/primary-antibodies/rpl26rpl26l-antibody-epr28600-70-ab325434'>ab325434</a>) at 1/1000 dilution

Lane 1:

HEK-293 (human embryonic kidney epithelial cell) whole cell lysate at 10 µg

Lane 2:

<a href='/products/primary-antibodies/rpl26rpl26l-antibody-epr28600-70-ab325434'>ab325434</a> at 1/30 IP in HEK-293 (human embryonic kidney epithelial cell) whole cell lysate

Lane 3:

Rabbit monoclonal IgG (<a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of <a href='/products/primary-antibodies/rpl26rpl26l-antibody-epr28600-70-ab325434'>ab325434</a> in HEK-293 whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution

Observed band size: 17 kDa

false

Exposure time: 180s

• IP

Supplier Data

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

POLE was immunoprecipitated from 0.35 mg parental HAP1(HOWT01) (human chronic myelogenous leukemia near-haploid cell ) whole cell lysate with ab318968 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab318968 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : parental HAP1(HOWT01) (human chronic myelogenous leukemia near-haploid cell ) whole cell lysate
Lane 2 : ab318968 IP in parental HAP1(HOWT01) (human chronic myelogenous leukemia near-haploid cell ) whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab318968 in parental HAP1(HOWT01) whole cell lysate

The bands beneath the target band between 100 kDa and 260 kDa are likely to be degraded target fragments.

All lanes:

Immunoprecipitation - Anti-POLE antibody [EPR28639-64] (<a href='/products/primary-antibodies/pole-antibody-epr28639-64-ab318968'>ab318968</a>) at 1/30 dilution

Lanes 1 - 2:

parental HAP1(HOWT01) (human chronic myelogenous leukemia near-haploid cell ) whole cell lysate at 10 µg

Lanes 4 - 5:

POLE Knockout HAP1(HO010404) whole cell lysate at 10 µg

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution

false

Exposure time: 6s

• IP

Lab

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

CD5 was immunoprecipitated from 0.35 mg MOLT-4 (human lymphoblastic leukemia T lymphoblast) whole cell lysate with ab325643 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab325643 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : MOLT-4 (human lymphoblastic leukemia T lymphoblast) whole cell lysate
Lane 2 : ab325643 IP in MOLT-4 (human lymphoblastic leukemia T lymphoblast) whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab325643 in MOLT-4 whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 76 seconds.

All lanes:

Immunoprecipitation - Anti-CD5 antibody [RM1352] (ab325643) at 1/1000 dilution

Lane 1:

MOLT-4 (human lymphoblastic leukemia T lymphoblast) whole cell lysate at 10 µg

Lane 2:

ab325643 at 1/30 IP in MOLT-4 (human lymphoblastic leukemia T lymphoblast) whole cell lysate

Lane 3:

Rabbit monoclonal IgG (<a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of ab325643 in MOLT-4 whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution

Observed band size: 67 kDa

false

Exposure time: 76s

• IP

Supplier Data

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

PAI1 was immunoprecipitated from 0.35 mg HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate with ab318286 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab318286 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate
Lane 2 : ab318286 IP in HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab318286 in HepG2 whole cell lysate

All lanes:

Immunoprecipitation - Anti-PAI1 antibody [RM1209] (<a href='/products/primary-antibodies/pai1-antibody-rm1209-ab318286'>ab318286</a>) at 1/30 dilution

All lanes:

HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution

false

Exposure time: 67s

• IP

Supplier Data

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

IL-2 was immunoprecipitated from 0.35 mg Jurkat (human T cell leukemia T lymphocyte from peripheral blood) whole cell lysate with ab317331 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab317331 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : Jurkat (human T cell leukemia T lymphocyte from peripheral blood) whole cell lysate
Lane 2 : ab317331 IP in Jurkat (human T cell leukemia T lymphocyte from peripheral blood) whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab317331 in Jurkat whole cell lysate

All lanes:

Immunoprecipitation - Anti-IL-2 antibody [RM1124] (<a href='/products/primary-antibodies/il-2-antibody-rm1124-ab317331'>ab317331</a>) at 1/30 dilution

All lanes:

Jurkat (human T cell leukemia T lymphocyte from peripheral blood) whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution

false

Exposure time: 180s

• IP

Supplier Data

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

RIP was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate with ab300617 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab300617 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP)(ab131366) was used at 1/5000 dilution.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-RIP antibody [EPR24883-85] (<a href='/products/primary-antibodies/rip-antibody-epr24883-85-ab300617'>ab300617</a>) at 1/30 dilution

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 10 µg

Lane 2:

<a href='/products/primary-antibodies/rip-antibody-epr24883-85-ab300617'>ab300617</a> IP in HeLa whole cell lysate

Lane 3:

Rabbit monoclonal IgG (<a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of <a href='/products/primary-antibodies/rip-antibody-epr24883-85-ab300617'>ab300617</a> in HeLa whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution

Predicted band size: 75 kDa

Observed band size: 75 kDa

false

Exposure time: 41s

• IP

Lab

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

KMT6 / EZH2 was immunoprecipitated from 0.35mg lysate HEK-293 (human embryonic kidney epithelial cell) whole cell lysate with ab227648 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab227648 at 1/1000 dilution. Capture antibody, 1 : 30 dilution (2μg in 0.35mg lysates) VeriBlot for IP secondary antibody (HRP) (ab131366) was used at 1/5000 dilution.

To minimize protein degradation, cells were lysed immediately after harvest and then applied for Immunoprecipitation as soon as possible.

Rabbit monoclonal IgG (ab172730) Isotype Control was used instead of ab185971 in HEK-293 whole cell lysate (Lane 3).

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

To minimize protein degradation, cells were lysed immediately after harvest and then applied for Immunoprecipitation as soon as possible.

All lanes:

Immunoprecipitation - Anti-KMT6 / EZH2 antibody [SP129] (<a href='/products/primary-antibodies/kmt6-ezh2-antibody-sp129-ab227648'>ab227648</a>) at 1/30 dilution

Lane 1:

HEK-293 (human embryonic kidney epithelial cell) whole cell lysate 10 μg

Lane 2:

HEK-293 (human embryonic kidney epithelial cell) whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution

Observed band size: 85 kDa

false

Exposure time: 32s

• IP

Lab

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

Annexin V/ANXA5 was immunoprecipitated from 0.35 mg 293T (human embryonic kidney epithelial cell) whole cell lysate with ab325388 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab325388 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : 293T (human embryonic kidney epithelial cell) whole cell lysate
Lane 2 : ab325388 at 1/30 IP in 293T (human embryonic kidney epithelial cell) whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab325388 in 293T whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 10 seconds.

All lanes:

Immunoprecipitation - Anti-Annexin V/ANXA5 antibody [RM1317] (<a href='/products/primary-antibodies/annexin-v-anxa5-antibody-rm1317-ab325388'>ab325388</a>) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) whole cell lysate at 10 µg

Lane 2:

<a href='/products/primary-antibodies/annexin-v-anxa5-antibody-rm1317-ab325388'>ab325388</a> at 1/30 IP in 293T (human embryonic kidney epithelial cell) whole cell lysate

Lane 3:

Rabbit monoclonal IgG (<a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of <a href='/products/primary-antibodies/annexin-v-anxa5-antibody-rm1317-ab325388'>ab325388</a> in 293Twhole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution

Observed band size: 35 kDa

false

Exposure time: 10s

• IP

Supplier Data

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

CD37 was immunoprecipitated from 0.35 mg Daudi (human Burkitt's lymphoma lymphoblast) whole cell lysate with ab323574 at 1/1000 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab323574 at 1/1000 dilution.

Blocking and dilution buffer and concentration : 5% NFDM/TBST

All lanes:

Immunoprecipitation - Anti-CD37 antibody [RM1306] (<a href='/products/primary-antibodies/cd37-antibody-rm1306-ab323574'>ab323574</a>) at 1/1000 dilution

Lane 1:

Daudi (human Burkitt's lymphoma lymphoblast) whole cell lysate at 10 µg

Lane 2:

<a href='/products/primary-antibodies/cd37-antibody-rm1306-ab323574'>ab323574</a> at 1/30 IP in Daudi (human Burkitt's lymphoma lymphoblast) whole cell lysate at 10 µg

Lane 3:

Rabbit monoclonal IgG (<a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of <a href='/products/primary-antibodies/cd37-antibody-rm1306-ab323574'>ab323574</a> in Daudi whole cell lysate at 10 µg

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution

Observed band size: 40 kDa

false

Exposure time: 6s

• IP

Lab

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

NUP214 was immunoprecipitated from 0.35 mg HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate with ab325238 at 1/30 dilution (2 μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab325238 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : ab325238 IP in HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab325238 in HeLa whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 180 seconds.

All lanes:

Immunoprecipitation - Anti-NUP214 antibody [EPR29100-623] (<a href='/products/primary-antibodies/nup214-antibody-epr29100-623-ab325238'>ab325238</a>) at 1/1000 dilution

Lane 1:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 10 µg

Lane 2:

<a href='/products/primary-antibodies/nup214-antibody-epr29100-623-ab325238'>ab325238</a> at 1/30 IP in HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate

Lane 3:

Rabbit monoclonal IgG (<a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of <a href='/products/primary-antibodies/nup214-antibody-epr29100-623-ab325238'>ab325238</a> in HeLa whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution

Observed band size: 268 kDa

false

Exposure time: 180s

• IP

Supplier Data

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

ALDH7A1 was immunoprecipitated from 0.35 mg of human liver tissue lysate with the antibody at 1/20 dilution. Western blot was performed from the immunoprecipitated using antibody at 1/1000 dilution.

All lanes:

Immunoprecipitation - Anti-ALDH7A1 antibody [EP1935Y] (<a href='/products/primary-antibodies/aldh7a1-antibody-ep1935y-ab53278'>ab53278</a>) at 1/20 dilution

Lane 1:

Human liver tissue lysate (Input) at 10 µg

Lane 2:

Human liver tissue lysate (+)

Lane 3:

Rabbit monoclonal IgG (<a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of <a href='/products/primary-antibodies/aldh7a1-antibody-ep1935y-ab53278'>ab53278</a> in human liver tissue lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution

false

• IP

Lab

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

SHARP2/DEC1 was immunoprecipitated from 0.35 mg HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate with ab325919 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab325919 at 1/325919 1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : ab325919 IP in HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab325919 in HeLa whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 180 seconds.

To minimize protein degradation, cells were lysed immediately after harvest and then applied for Immunoprecipitation as soon as possible.

All lanes:

Immunoprecipitation - Anti-SHARP2/DEC1 antibody [EPR29211-84] (<a href='/products/primary-antibodies/sharp2-dec1-antibody-epr29211-84-ab325919'>ab325919</a>) at 1/1000 dilution

Lane 1:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 10 µg

Lane 2:

<a href='/products/primary-antibodies/sharp2-dec1-antibody-epr29211-84-ab325919'>ab325919</a> at 1/30 IP in HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate

Lane 3:

Rabbit monoclonal IgG (<a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of <a href='/products/primary-antibodies/sharp2-dec1-antibody-epr29211-84-ab325919'>ab325919</a> in HeLa whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution

Observed band size: 49 kDa

false

Exposure time: 180s

• IP

Supplier Data

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

POLE was immunoprecipitated from 0.35 mg K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate with ab318968 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab318968 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate
Lane 2 : ab318968 IP in K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab318968 in K-562 whole cell lysate

The bands beneath the target band between 100 kDa and 260 kDa are likely to be degraded target fragments.

All lanes:

Immunoprecipitation - Anti-POLE antibody [EPR28639-64] (<a href='/products/primary-antibodies/pole-antibody-epr28639-64-ab318968'>ab318968</a>) at 1/30 dilution

Lanes 1 - 2:

K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate at 10 µg

Lanes 4 - 5:

F9 (mouse embryonal carcinoma epithelial cell) whole cell lysate at 10 µg

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution

false

Exposure time: 10s

• IP

Lab

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

BICD2 was immunoprecipitated from 0.35 mg HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate with ab325398 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab325398 at 1/325398 1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : ab325398 IP in HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab325398 in HeLa whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 50 seconds.

All lanes:

Immunoprecipitation - Anti-BICD2 antibody [EPR30244-565] (<a href='/products/primary-antibodies/bicd2-antibody-epr30244-565-ab325398'>ab325398</a>) at 1/1000 dilution

Lane 1:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 10 µg

Lane 2:

<a href='/products/primary-antibodies/bicd2-antibody-epr30244-565-ab325398'>ab325398</a> at 1/30 IP in HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate

Lane 3:

Rabbit monoclonal IgG (<a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of <a href='/products/primary-antibodies/bicd2-antibody-epr30244-565-ab325398'>ab325398</a> in HeLa whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution

Observed band size: 100 kDa

false

Exposure time: 50s

• IP

Lab

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

NOX5 was immunoprecipitated from 0.35 mg PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate with ab325647 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab325647 at 1/325647 1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : ab325647 IP in PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab325647 in PC-3 whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 15 seconds.

All lanes:

Immunoprecipitation - Anti-NOX5 antibody [EPR29814-35] (<a href='/products/primary-antibodies/nox5-antibody-epr29814-35-ab325647'>ab325647</a>) at 1/1000 dilution

Lane 1:

PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate at 10 µg

Lane 2:

<a href='/products/primary-antibodies/nox5-antibody-epr29814-35-ab325647'>ab325647</a> at 1/30 IP in PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate

Lane 3:

Rabbit monoclonal IgG (<a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of <a href='/products/primary-antibodies/nox5-antibody-epr29814-35-ab325647'>ab325647</a> in PC-3 whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution

Observed band size: 75 kDa

false

Exposure time: 15s

• IP

Supplier Data

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

Histone H3(acetyl K4+K9+K14+K18+K23+K27) was immunoprecipitated from 0.35 mg HeLa (human cervical adenocarcinoma epithelial cell) treated with 500ng/ml TSA for 4h whole cell lysate 10 µg with ab300641 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab300641 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366) was used at 1/5000 dilution.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-Histone H3 (acetyl K4+K9+K14+K18+K23+K27) antibody [RM1045] (<a href='/products/primary-antibodies/histone-h3-acetyl-k4k9k14k18k23k27-antibody-rm1045-ab300641'>ab300641</a>) at 1/30 dilution

Lane 1:

HeLa (human cervical adenocarcinoma epithelial cell) (Input) treated with 500ng/ml TSA for 4h whole cell lysate at 10 µg

Lane 2:

<a href='/products/primary-antibodies/histone-h3-acetyl-k4k9k14k18k23k27-antibody-rm1045-ab300641'>ab300641</a> IP in HeLa treated with 500ng/ml TSA for 4h whole cell lysate

Lane 3:

Rabbit monoclonal IgG (<a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of <a href='/products/primary-antibodies/histone-h3-acetyl-k4k9k14k18k23k27-antibody-rm1045-ab300641'>ab300641</a> in HeLa treated with 500ng/ml TSA for 4h whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution

Predicted band size: 15 kDa

Observed band size: 15 kDa

false

Exposure time: 100s

• IP

Lab

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

ATP13A2 was immunoprecipitated from 0.35 mg HEK-293 (human embryonic kidney epithelial cell) whole cell lysate with ab322735 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab322735 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : HEK-293 (human embryonic kidney epithelial cell) whole cell lysate
Lane 2 : ab322735 at 1/30 IP in HEK-293 (human embryonic kidney epithelial cell) whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab322735 in HEK-293 whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 180 seconds.

All lanes:

Immunoprecipitation - Anti-ATP13A2 antibody [MJF-D29756-28] (<a href='/products/primary-antibodies/atp13a2-antibody-mjf-d29756-28-ab322735'>ab322735</a>) at 1/1000 dilution

Lane 1:

HEK-293 (human embryonic kidney epithelial cell) whole cell lysate at 10 µg

Lane 2:

<a href='/products/primary-antibodies/atp13a2-antibody-mjf-d29756-28-ab322735'>ab322735</a> at 1/30 IP in HEK-293 (human embryonic kidney epithelial cell) whole cell lysate

Lane 3:

Rabbit monoclonal IgG (<a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of <a href='/products/primary-antibodies/atp13a2-antibody-mjf-d29756-28-ab322735'>ab322735</a> in HEK-293 whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution

Observed band size: 150 kDa

false

Exposure time: 180s

• IP

Lab

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

Zfp281/ZNF281 was immunoprecipitated from 0.35 mg U-2 OS (human bone osteosarcoma epithelial cell) whole cell lysate with ab325790 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab325790 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : U-2 OS (human bone osteosarcoma epithelial cell) whole cell lysate
Lane 2 : ab325790 IP in U-2 OS (human bone osteosarcoma epithelial cell) whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab325790 in U-2 OS whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 41 seconds.

To minimize protein degradation, lysates were made fresh.

All lanes:

Immunoprecipitation - Anti-Zfp281/ZNF281 antibody [EPR30593-518] (<a href='/products/primary-antibodies/zfp281-znf281-antibody-epr30593-518-ab325790'>ab325790</a>) at 1/1000 dilution

Lane 1:

U-2 OS (human bone osteosarcoma epithelial cell) whole cell lysate at 10 µg

Lane 2:

<a href='/products/primary-antibodies/zfp281-znf281-antibody-epr30593-518-ab325790'>ab325790</a> at 1/30 IP in U-2 OS (human bone osteosarcoma epithelial cell) whole cell lysate

Lane 3:

Rabbit monoclonal IgG (<a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of <a href='/products/primary-antibodies/zfp281-znf281-antibody-epr30593-518-ab325790'>ab325790</a> in U-2 OS whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution

Observed band size: 100 kDa

false

Exposure time: 41s

• IP

Lab

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

AGO2(phospho S387) was immunoprecipitated from 0.35 mg HEK293T cells were co-transfected with human AGO2 (His-tagged) and human AKT3 (EGFP-tagged) expression vectors, an upstream kinase used to enhance AGO2 phosphorylation at S387, whole cell lysate with ab326569 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab326569 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : HEK293T cells were co-transfected with human AGO2 (His-tagged) and human AKT3 (EGFP-tagged) expression vectors, an upstream kinase used to enhance AGO2 phosphorylation at S387, whole cell lysate
Lane 2 : ab326569 IP in HEK293T cells were co-transfected with human AGO2 (His-tagged) and human AKT3 (EGFP-tagged) expression vectors, an upstream kinase used to enhance AGO2 phosphorylation at S387, whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab326569 in HEK293T cells were co-transfected with human AGO2 (His-tagged) and human AKT3 (EGFP-tagged) expression vectors, an upstream kinase used to enhance AGO2 phosphorylation at S387, whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 180 seconds.

All lanes:

Immunoprecipitation - Anti-AGO2(phospho S387) antibody [EPR29226-168] (ab326569) at 1/1000 dilution

Lane 1:

HEK293T cells were co-transfected with human AGO2 (His-tagged) and human AKT3 (EGFP-tagged) expression vectors, an upstream kinase used to enhance AGO2 phosphorylation at S387, whole cell lysate at 10 µg

Lane 2:

ab326569 at 1/30 IP in HEK293T cells were co-transfected with human AGO2 (His-tagged) and human AKT3 (EGFP-tagged) expression vectors, an upstream kinase used to enhance AGO2 phosphorylation at S387, whole cell lysate

Lane 3:

Rabbit monoclonal IgG (<a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of ab326569 in HEK293T cells were co-transfected with human AGO2 (His-tagged) and human AKT3 (EGFP-tagged) expression vectors, an upstream kinase used to enhance AGO2 phosphorylation at S387, whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution

Observed band size: 97 kDa

false

Exposure time: 180s

• IP

Lab

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

POLR3A was immunoprecipitated from 0.35 mg HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate with ab325791 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab325791 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : ab325791 IP in HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab325791 in HeLa whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 180 seconds.

All lanes:

Immunoprecipitation - Anti-POLR3A antibody [EPR30480-88] (<a href='/products/primary-antibodies/polr3a-antibody-epr30480-88-ab325791'>ab325791</a>) at 1/1000 dilution

Lane 1:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 25 µg

Lane 2:

<a href='/products/primary-antibodies/polr3a-antibody-epr30480-88-ab325791'>ab325791</a> at 1/30 IP in HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate

Lane 3:

Rabbit monoclonal IgG (<a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of <a href='/products/primary-antibodies/polr3a-antibody-epr30480-88-ab325791'>ab325791</a> in HeLa whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution

Observed band size: 155 kDa

false

Exposure time: 180s

• IP

Lab

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

IL-12RB1 was immunoprecipitated from 0.35 mg HDLM-2 (human Hodgkin lymphoma cell) whole cell lysate with ab325502 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab325502 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : HDLM-2 (human Hodgkin lymphoma cell) whole cell lysate
Lane 2 : ab325502 IP in HDLM-2 (human Hodgkin lymphoma cell) whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab325502 in HDLM-2 whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 180 seconds.

All lanes:

Immunoprecipitation - Anti-IL-12RB1 antibody [EPR28354-118] (<a href='/products/primary-antibodies/il-12rb1-antibody-epr28354-118-ab325502'>ab325502</a>)

Lane 1:

HDLM-2 (human Hodgkin lymphoma cell) whole cell lysate at 10 µg

Lane 2:

<a href='/products/primary-antibodies/il-12rb1-antibody-epr28354-118-ab325502'>ab325502</a> at 1/30 IP in HDLM-2 (human Hodgkin lymphoma cell) whole cell lysate

Lane 3:

Rabbit monoclonal IgG (<a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of <a href='/products/primary-antibodies/il-12rb1-antibody-epr28354-118-ab325502'>ab325502</a> in HDLM-2 whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution

Observed band size: 100 kDa

false

Exposure time: 180s

• IP

Supplier Data

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

CTRP6 was immunoprecipitated from 0.35 mg human placenta tissue lysate with ab300583 at 1/30 dilution (2 µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab300583 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP)(ab131366) was used at 1/5000 dilution.

All lanes:

Immunoprecipitation - Anti-CTRP6 antibody [EPR25750-35] (<a href='/products/primary-antibodies/ctrp6-antibody-epr25750-35-ab300583'>ab300583</a>) at 1/30 dilution

Lane 1:

Human placenta tissue lysate (Inset) at 10 µg

Lane 2:

<a href='/products/primary-antibodies/ctrp6-antibody-epr25750-35-ab300583'>ab300583</a> IP in Human placenta tissue lysate

Lane 3:

Rabbit monoclonal IgG (<a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of <a href='/products/primary-antibodies/ctrp6-antibody-epr25750-35-ab300583'>ab300583</a> in human placenta tissue lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution

Predicted band size: 29 kDa

Observed band size: 30 kDa

false

Exposure time: 3min

• IP

Lab

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

RYBP was immunoprecipitated from HEK-293 (human embryonic kidney epithelial cell) whole cell lysate 10 μg with ab185971 1/1000 dilution. Western blot was performed on the immunoprecipitate using ab185971 at 1/1000 dilution. Capture antibody, 1 : 30 dilution (2μg in 0.35mg lysates).

Rabbit monoclonal IgG (ab172730) Isotype Control was used instead of ab185971 in HEK-293 whole cell lysate (Lane 3).

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

To minimize protein degradation, cells were lysed immediately after harvest and then applied for Immunoprecipitation as soon as possible.

All lanes:

Immunoprecipitation - Anti-RYBP antibody [EPR13059(2)] - ChIP Grade (<a href='/products/primary-antibodies/rybp-antibody-epr130592-chip-grade-ab185971'>ab185971</a>) at 1/1000 dilution

Lane 1:

HEK-293 (human embryonic kidney epithelial cell) whole cell lysate (Low Expression Sample) 10 μg

Lane 2:

HEK-293 (human embryonic kidney epithelial cell) whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution

Observed band size: 32 kDa

false

Exposure time: 15s

• IP

Lab

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

c-Myb was immunoprecipitated from 0.35 mg MOLT-4 (human lymphoblastic leukemia T lymphoblast) fresh whole cell lysate with ab324970 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab324970 at 1/324970 1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : MOLT-4 (human lymphoblastic leukemia T lymphoblast) fresh whole cell lysate
Lane 2 : ab324970 IP in MOLT-4 (human lymphoblastic leukemia T lymphoblast) fresh whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab324970 in MOLT-4 whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 180 seconds.

All lanes:

Immunoprecipitation - Anti-c-Myb antibody [EPR27202-86] (<a href='/products/primary-antibodies/c-myb-antibody-epr27202-86-ab324970'>ab324970</a>) at 1/1000 dilution

Lane 1:

MOLT-4 (human lymphoblastic leukemia T lymphoblast) fresh whole cell lysate at 10 µg

Lane 2:

MOLT-4 (human lymphoblastic leukemia T lymphoblast) fresh whole cell lysate

Lane 3:

Rabbit monoclonal IgG (<a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of <a href='/products/primary-antibodies/c-myb-antibody-epr27202-86-ab324970'>ab324970</a> in MOLT-4 whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution

Observed band size: 75 kDa

false

Exposure time: 180s

• IP

Lab

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

n-Myc/MYCN was immunoprecipitated from 0.35 mg IMR-32 (human neuroblastoma neuroblast) whole cell lysate with ab325641 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab325641 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : IMR-32 (human neuroblastoma neuroblast) whole cell lysate
Lane 2 : ab325641 IP in IMR-32 (human neuroblastoma neuroblast) whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab325641 in IMR-32 whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 180 seconds.

All lanes:

Immunoprecipitation - Anti-n-Myc/MYCN antibody [EPR26107-153] (<a href='/products/primary-antibodies/n-myc-mycn-antibody-epr26107-153-ab325641'>ab325641</a>) at 1/1000 dilution

Lane 1:

IMR-32 (human neuroblastoma neuroblast) whole cell lysate at 10 µg

Lane 2:

<a href='/products/primary-antibodies/n-myc-mycn-antibody-epr26107-153-ab325641'>ab325641</a> at 1/30 IP in IMR-32 (human neuroblastoma neuroblast) whole cell lysate

Lane 3:

Rabbit monoclonal IgG (<a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of <a href='/products/primary-antibodies/n-myc-mycn-antibody-epr26107-153-ab325641'>ab325641</a> in IMR-32 whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution

Observed band size: 60 kDa

false

Exposure time: 180s

• IP

Lab

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

KDM4A / JHDM3A / JMJD2A was immunoprecipitated from 0.35 mg HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate with ab326104 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab326104 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : ab326104 IP in HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab326104 in HeLa whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 180 seconds.

To minimize protein degradation, cells (lanes 5-8) were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.

All lanes:

Immunoprecipitation - Anti-KDM4A / JHDM3A / JMJD2A antibody [EPR29602-146] (<a href='/products/primary-antibodies/kdm4a-jhdm3a-jmjd2a-antibody-epr29602-146-ab326104'>ab326104</a>) at 1/1000 dilution

Lane 1:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 10 µg

Lane 2:

<a href='/products/primary-antibodies/kdm4a-jhdm3a-jmjd2a-antibody-epr29602-146-ab326104'>ab326104</a> at 1/30 IP in HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate

Lane 3:

Rabbit monoclonal IgG (<a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of <a href='/products/primary-antibodies/kdm4a-jhdm3a-jmjd2a-antibody-epr29602-146-ab326104'>ab326104</a> in HeLa whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution

Observed band size: 150 kDa

false

Exposure time: 180s

• IP

Lab

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

SAP97 was immunoprecipitated from 0.35 mg SH-SY5Y (human neuroblastoma epithelial cell), whole cell lysate 10 µg with ab300481 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab300481 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-SAP97 antibody [EPR26467-123] (<a href='/products/primary-antibodies/sap97-antibody-epr26467-123-ab300481'>ab300481</a>) at 1/30 dilution

Lane 1:

SH-SY5Y (human neuroblastoma epithelial cell), whole cell lysate (Inset) at 10 µg

Lane 2:

<a href='/products/primary-antibodies/sap97-antibody-epr26467-123-ab300481'>ab300481</a> IP in SH-SY5Y whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution

Observed band size: 100 kDa

false

Exposure time: 5.5s

• IP

Lab

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

ATP13A2 was immunoprecipitated from 0.35 mg Saos-2 (human osteosarcoma epithelial cell) whole cell lysate with ab322735 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab322735 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : Saos-2 (human osteosarcoma epithelial cell) whole cell lysate
Lane 2 : ab322735 at 1/30 IP in Saos-2 (human osteosarcoma epithelial cell) whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab322735 in Saos-2 whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 102 seconds.

All lanes:

Immunoprecipitation - Anti-ATP13A2 antibody [MJF-D29756-28] (<a href='/products/primary-antibodies/atp13a2-antibody-mjf-d29756-28-ab322735'>ab322735</a>) at 1/1000 dilution

Lane 1:

Saos-2 (human osteosarcoma epithelial cell) whole cell lysate at 10 µg

Lane 2:

<a href='/products/primary-antibodies/atp13a2-antibody-mjf-d29756-28-ab322735'>ab322735</a> at 1/30 IP in Saos-2 (human osteosarcoma epithelial cell) whole cell lysate

Lane 3:

Rabbit monoclonal IgG (<a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of <a href='/products/primary-antibodies/atp13a2-antibody-mjf-d29756-28-ab322735'>ab322735</a> in Saos-2 whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution

Observed band size: 150 kDa

false

Exposure time: 102s

• IP

Lab

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

Purified ab250467 at 1 : 20 dilution (0.8μg) immunoprecipitating FXYD6/Plp in Human testis lysate.
Blocking Buffer and concentration : 5% NFDM/TBST.
Diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-FXYD6/Plp antibody [EPR9004(2)] (<a href='/products/primary-antibodies/fxyd6-plp-antibody-epr90042-ab181254'>ab181254</a>)

Lane 1:

Human testis lysate at 10 µg

Lane 2:

<a href='/products/primary-antibodies/fxyd6-plp-antibody-epr90042-bsa-and-azide-free-ab250467'>ab250467</a> + Human testis lysate

Lane 3:

Rabbit monoclonal IgG (<a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of <a href='/products/primary-antibodies/fxyd6-plp-antibody-epr90042-ab181254'>ab181254</a> in human testis lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

Predicted band size: 11 kDa

false

• IP

Lab

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

SMC1A was immunoprecipitated from 0.35 mg MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate with ab325922 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab325922 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : ab325922 IP in MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab325922 in MCF7 whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 15 seconds.

All lanes:

Immunoprecipitation - Anti-SMC1A antibody [EPR30532-7] (<a href='/products/primary-antibodies/smc1a-antibody-epr30532-7-ab325922'>ab325922</a>) at 1/1000 dilution

Lane 1:

MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 10 µg

Lane 2:

<a href='/products/primary-antibodies/smc1a-antibody-epr30532-7-ab325922'>ab325922</a> at 1/30 IP in MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate

Lane 3:

Rabbit monoclonal IgG (<a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of <a href='/products/primary-antibodies/smc1a-antibody-epr30532-7-ab325922'>ab325922</a> in MCF7 whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution

Observed band size: 160 kDa

false

Exposure time: 15s

• IP

Supplier Data

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

beta Catenin (phospho S552) was immunoprecipitated from 0.35 mg SK-N-MC (human brain epithelial cell) treated with 100 μM forskolin for 30 minutes whole cell lysate with ab314502 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab314502 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : SK-N-MC (human brain epithelial cell) treated with 100 μM forskolin for 30 minutes whole cell lysate

Lane 2 : ab314502 IP in SK-N-MC (human brain epithelial cell) treated with 100 μM forskolin for 30 minutes whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab314502 in SK-N-MC treated with 100 μM forskolin for 30 minutes whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-beta Catenin (phospho S552) antibody [EPR28411-7] (<a href='/products/primary-antibodies/beta-catenin-phospho-s552-antibody-epr28411-7-ab314502'>ab314502</a>) at 1/30 dilution

All lanes:

SK-N-MC (human brain epithelial cell) treated with 100&micro;M forskolin for 30 minutes whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution

false

Exposure time: 145s

• IP

Lab

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

was immunoprecipitated from 0.35 mg THP-1 (human monocytic leukemia monocyte) whole cell lysate with ab325712 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab325712 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : THP-1 (human monocytic leukemia monocyte) whole cell lysate
Lane 2 : ab325712 IP in THP-1 (human monocytic leukemia monocyte) whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab325712 in THP-1 whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 24 seconds.

All lanes:

Immunoprecipitation - Anti-FcRgamma antibody [EPR30963-69] (<a href='/products/primary-antibodies/fcrgamma-antibody-epr30963-69-ab325712'>ab325712</a>) at 1/1000 dilution

Lane 1:

THP-1 (human monocytic leukemia monocyte) whole cell lysate at 5 µg

Lane 2:

<a href='/products/primary-antibodies/fcrgamma-antibody-epr30963-69-ab325712'>ab325712</a> at 1/30 IP in THP-1 (human monocytic leukemia monocyte) whole cell lysate

Lane 3:

Rabbit monoclonal IgG (<a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of <a href='/products/primary-antibodies/fcrgamma-antibody-epr30963-69-ab325712'>ab325712</a> in THP-1 whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution

Observed band size: 10 kDa

false

Exposure time: 24s

• IP

Supplier Data

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (AB131366)

Collybistin was immunoprecipitated from 0.35 mg SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate with ab316963 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab316963 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate
Lane 2 : ab316963 IP in SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab316963 in SH-SY5Y whole cell lysate

All lanes:

Immunoprecipitation - Anti-Collybistin antibody [EPR28024-32] (<a href='/products/primary-antibodies/collybistin-antibody-epr28024-32-ab316963'>ab316963</a>) at 1/30 dilution

All lanes:

SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/5000 dilution

false

Exposure time: 84s