TMB ELISA底物(Highest Sensitivity) (ab171522)
概述
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产品名称
TMB ELISA底物(Highest Sensitivity)
参阅全部 ELISA substrate 试剂 -
经测试应用
适用于: ELISAmore details
不适用于: IHC-Fr or IHC-P -
常规说明
Abcam’s TMB ELISA Substrate (Highest Sensitive) detects horseradish peroxidase (HRP) activity and contains 3,3’,5,5’-tetramethylbenzidine in a mildly acidic buffer. The substrate is supplied as a ready to use solution. Unreacted substrate should be colorless or very light yellow in appearance. When this substrate system is reacted with peroxidase, a soluble blue reaction product is obtained. The reaction can be stopped using appropriate stop solution, producing a soluble yellow or soluble blue reaction product, depending upon the stop reagent used, which is stable for at least 1 hour. ab171522 is not recommended for membrane or immunohistochemical applications that require a precipitating reaction product.
Product Use:
ab171522 is supplied as a ready to use solution. The product should be allowed to equilibrate to room temperature (25°C) prior to use. For microwell applications, 100 µL of substrate solution is added to each well. A soluble blue reaction product develops which can be read at 370 nm or in a range of 620 nm to 650 nm. For best results, sample absorbance values should be monitored and read before absorbance values exceed 2.0 OD units. In endpoint assays, the substrate reaction can be stopped using equal volumes of 1 N HCl, 0.6 N sulfuric acid, or one of the stop solutions (ab171529 and ab171531). Addition of acid turns the blue color to yellow and stops the enzymatic reaction. In the case of the 650 nm Stop Solutions for TMB Substrate (ab171531), the blue color does not change. Since stopping the reaction increases sample absorbance values approximately three fold, unless using the 650 nm Stop Solutions for TMB Substrate (ab171531), sample OD values should be monitored and substrate reaction stopped when values reach approximately 0.7 OD units. After stopping with the 450 nm Stop Solutions for TMB Substrate (ab171529), a soluble yellow product develops which is read in the 450 nm range. Stopping with the 650 nm Stop Solutions for TMB Substrate (ab171531) produces a soluble blue product which is read in the 650 nm range. Dilution of the substrate is not recommended. To reduce the intensity of a reaction, it is recommended that the antibodies or conjugates be diluted.Storage Instructions:
Exposure to direct sunlight and other UV sources should be avoided due to the light sensitive nature of the TMB molecule.
性能
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形式
Liquid -
存放说明
Store at +4°C. Please see notes section. -
存储溶液
pH: 1
Constituents: 0.05% TMB, 79% Water, 0.1% Hydrogen peroxide -
Concentration information loading...
相关产品
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Related Products
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
- 450 nm Stop Solution for TMB Substrate (ab171529)
- 650 nm Stop Solution for TMB Substrate (ab171531)
- Phosphate Buffered Saline (PBS) Casein ELISA Reagent (ab171532)
- Tris Buffered Saline (TBS) Casein ELISA Reagent (ab171533)
- Immunoassay Blocking Buffer (ab171534)
- Immunoassay Blocking (BSA Free) (ab171535)
- AP Stability Conjugate Reagent (ab171536)
- HRP Stability Conjugate Reagent (ab171537)
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab171522于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ELISA |
Use at an assay dependent concentration.
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说明 |
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ELISA
Use at an assay dependent concentration. |
图片
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (14)
ab171522 被引用在 14 文献中.
- Wang Y et al. An automatic immuno-microfluidic system integrating electrospun polystyrene microfibrous reactors for rapid detection of salivary cortisol. iScience 26:107820 (2023). PubMed: 37752956
- Sun S et al. Respiratory mucosal vaccination of peptide-poloxamine-DNA nanoparticles provides complete protection against lethal SARS-CoV-2 challenge. Biomaterials 292:121907 (2023). PubMed: 36436305
- Li W et al. Stability of SARS-CoV-2-Encoded Proteins and Their Antibody Levels Correlate with Interleukin 6 in COVID-19 Patients. mSystems 7:e0005822 (2022). PubMed: 35582921
- Pinkaew D et al. Fortilin interacts with TGF-ß1 and prevents TGF-ß receptor activation. Commun Biol 5:157 (2022). PubMed: 35197550
- Bakkers MJG et al. CD164 is a host factor for lymphocytic choriomeningitis virus entry. Proc Natl Acad Sci U S A 119:e2119676119 (2022). PubMed: 35235462