Phosphate Buffered Saline (PBS) Casein ELISA试剂盒(ab171532)
概述
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产品名称
Phosphate Buffered Saline (PBS) Casein ELISA试剂盒
参阅全部 ELISA buffer 试剂 -
经测试应用
适用于: ELISAmore details
不适用于: IHC-Fr or IHC-P -
常规说明
Abcam’s Phosphate Buffered Saline (PBS) Casein ELISA Reagent consists of many phosphoserine groups that function to bind Ca2+. The solution is ready for dilution to specific application requirements. ab171532 contributes to the minimization of non-specific interactions of the secondary reagents with each other and with the primary solid phase binding sites. The high blocking efficiency of casein is due to its content of small molecular weight proteins. Phosphate Buffered Saline (PBS), pH 7.4, is a poor buffer above pH 7.5. Phosphate buffer should not be used in assays where competition for phosphate groups or complex formation with a metal ion is essential for the enzyme activation. Phosphate ions will inhibit carboxypeptidiase, carboxylase, urease, muscle diaminase, formase and phosphoglucomutase.
Storage Instructions:
This product should be clear and colorless in appearance. To minimize the risk of contamination, a fresh working solution should be prepared each day or store unused working solutions at the recommended storage temperature. If proper technique is not performed the possibility of introducing contaminants is always possible.Product Use:
ab171532 should be diluted with reagent quality water. To produce a 1X working solution, dilute 1 mL of Phosphate Buffered Saline (PBS) Casein ELISA Reagent in 4 mL reagent quality water. To use as a blocking reagent, treat target surface by covering completely with 1X solution. To use as a diluent, dilute antigen/antibody to desired concentration in 1X solution and treat surface by covering completely. When using 1X solution for coating plates, best results are obtained following an overnight incubation at +4°C.
性能
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形式
Liquid -
存放说明
Store at room temperature. -
存储溶液
pH: 7
Constituent: Casein -
Concentration information loading...
相关产品
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应用
The Abpromise guarantee
Abpromise™承诺保证使用ab171532于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ELISA |
Use at an assay dependent concentration.
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说明 |
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ELISA
Use at an assay dependent concentration. |
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab171532 被引用在 1 文献中.
- Gong S et al. Antibody Light Chains: Key to Increased Monoclonal Antibody Yields in Expi293 Cells? Antibodies (Basel) 11:N/A (2022). PubMed: 35645210