AB109209

Serum抗体Purification试剂盒(Protein A)

Name: Serum抗体Purification试剂盒(Protein A) (ab109209)| Abcam中文官网
Brand: Abcam Limited
SKU: AB109209
Price: 4622 CNY
Availability: InStock

Serum Antibody Purification Kit (Protein A)

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(2 Publications)

Serum Antibody Purification Kit (Protein A) (ab109209) is designed to purify IgG fractions from serum. The kit uses Protein A which has a high affinity for Fc regions of IgG molecules from a variety of species.

- All reagents (including Protein A resin) are ready-to-use as supplied.
- Up to 20 mg of antibody can be purified in each run.
- Compatible with our Lightning-Link^® Antibody Conjugation Kits and Oligonucleotide Conjugation Kit range.

关键信息

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产品详情

Serum Antibody Purification Kit (Protein A) (ab109209) is prepared by coupling highly purified protein A to agarose beads and can therefore be used to purify IgG fractions from both serum and ascites fluid.

The antibody is captured on the resin and unwanted substances are removed by a simple wash procedure. The purified product is then eluted and neutralized.

The components of ab109209 are fully compatible with our Lightning-Link® Antibody Conjugation Kits and our Oligonucleotide Conjugation Kit. However they are not compatible with our nanoparticle Antibody conjugation kits (Gold, Latex, Europium and Magnetic). To purify antibodies for use with our nanoparticle conjugation kits, please use our Antibody Purification Kit - Nanoparticles (ab204909).

ab109209 is not suitable for goat antibody purification. Please refer to the protocol booklet for more information about suitable and unsuitable species antibodies.

This product is manufactured by Expedeon, an Abcam company, and was previously called AbSelect™ A Serum Antibody Purification System. 863-0500 is the same as the 3 tests size. 863-0030 is the same as the 1 test size.

ab109209 contains 1-3 columns, and each can purify up to 20mg of antibody per run.

The volume of sample required will depend on the host species (See Table below):

Species	Normal range IgG(mg/ml)	Suitable Volumes for Product(ml)
Rabbit	12 - 15	1.3 - 1.7
Human	7 - 23	0.9 - 2.9
Mouse	2 - 5	4 - 10
Sheep/Goat	18 - 24	0.8 - 1.1
Rat	5 - 7	2.9 - 4
Ascites Fluid	0.5 - 5	4 - 40

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规格

{ "values": { "3Test": { "sellingSize": "3 Test", "publicAssetCode":"ab109209-3Test", "assetComponentDetails": [ { "size":"1 x 5 mL", "name":"Neutralizer Buffer", "number":"AB109209-CMP05" }, { "size":"1 x 18 mL", "name":"Binding Buffer (10X)", "number":"AB109209-CMP02" }, { "size":"1 x 75 mL", "name":"Wash Buffer", "number":"AB109209-CMP06" }, { "size":"1 x 15 mL", "name":"Elution Buffer", "number":"AB109209-CMP04" }, { "size":"3 x 1 Unit", "name":"Purification Column", "number":"AB109209-CMP03" }, { "size":"3 x 5 mL", "name":"Protein A resin", "number":"AB109209-CMP01" } ] }, "1Test": { "sellingSize": "1 Test", "publicAssetCode":"ab109209-1Test", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"Wash Buffer", "number":"AB109209-CMP06", "productcode":"" }, { "size":"1 x 5 mL", "name":"Protein A resin", "number":"AB109209-CMP01", "productcode":"AB270308" }, { "size":"1 x 1 Unit", "name":"Purification Column", "number":"AB109209-CMP03", "productcode":"" }, { "size":"1 x 5 mL", "name":"Elution Buffer", "number":"AB109209-CMP04", "productcode":"" }, { "size":"1 x 2 mL", "name":"Neutralizer Buffer", "number":"AB109209-CMP05", "productcode":"" }, { "size":"1 x 6 mL", "name":"Binding Buffer (10X)", "number":"AB109209-CMP02", "productcode":"" } ] } } }

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性能和储存信息

运输条件

Ambient - Cannot Ship with Ice

储存信息

+4°C

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产品实验方案

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靶点信息

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of clinical microbiology 60:e0023622 PubMed35652310

2022

Validation of an Indirect Immunofluorescence Assay and Commercial Q Fever Enzyme-Linked Immunosorbent Assay for Use in Macropods.

Applications

Unspecified application

Species

Unspecified reactive species

Anita Tolpinrud,John Stenos,Anne-Lise Chaber,Joanne M Devlin,Catherine Herbert,An Pas,Magdalena Dunowska,Mark A Stevenson,Simon M Firestone

Inflammopharmacology 25:643-651 PubMed28528362

2017

Budesonide, fluticasone propionate, and azithromycin do not modulate the membrane vesicle release by THP-1 macrophages and respiratory pathogens during macrophage infection.

Applications

Unspecified application

Species

Unspecified reactive species

Charlotte Volgers,Gert E Grauls,Pauline H M Hellebrand,Paul H M Savelkoul,Frank R M Stassen

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For licensing inquiries, please contact partnerships@abcam.com

Serum抗体Purification试剂盒(Protein A)

Serum Antibody Purification Kit (Protein A)

关键信息

产品详情