Streptavidin (HRP) (ab7403)
Key features and details
- Expression system: Native
- Purity: > 95% n/a
- Active: Yes
- Suitable for: Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, ICC, WB
描述
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产品名称
Streptavidin (HRP)
参阅全部 Streptavidin 蛋白酶 -
生物活性
Binds to Biotin. Dissociation constant has not been measured.
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纯度
> 95 % n/a.
Streptavidin-HRP was prepared from chromatographically purified streptavidin. -
表达系统
Native -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Native -
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种属
Streptomyces avidinii
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偶联物
HRP
相关产品
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Substrate reagent
技术指标
Our Abpromise guarantee covers the use of ab7403 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Dot blot
ELISA
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunohistochemistry (Frozen sections)
Immunomicroscopy
Immunocytochemistry
Western blot
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形式
Liquid -
补充说明
Horseradish peroxidase is conjugated to the streptavidin tetramer at ~1:1 molar ratio.
This product has been assayed against 1.0 µg of Biotinylated IgG in a standard capture ELISA using a peroxidase substrate as ABTS ab142041 as a substrate for 30 minutes at room temperature. A working dilution of 1:15,000 to 1:60,000 of the reconstitution concentration is suggested for this product. Optimal titers for other applications should be determined by the researcher.
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Preservative: 0.01% Gentamicin sulphate
Constituents: 0.424% Tripotassium orthophosphate, 0.88% Sodium chloride, 1% BSAThis product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
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别名
- SA V1
- SA V2
- strepavidin
see all -
相关性
Streptavidin is a tetrameric protein purified from Streptomyces sp. that binds very tightly to the vitamin biotin with a Kd of ~ 10-14 mol/l. The high affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits. -
细胞定位
Cytoplasmic
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Streptavidin (HRP) (ab7403)
IHC image of Histone H1 staining in a section of formalin-fixed paraffin-embedded [human normal colon]*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was then incubated with ab11080, 1/1000 dilution, for 15 mins at room temperature. A goat anti-mouse biotinylated secondary antibody (ab6788, 1/1000 dilution), was used to detect the primary, and visualized using an HRP conjugated ABC system. Streptavidin HRP was used, ab7403 at a 1/10000 dilution. DAB was used as the chromogen (ab103723), diluted 1/100 and incubated for 10min at room temperature. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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ELISA - Streptavidin (HRP) (ab7403)The wells were coated with ab200699 at 1µg/ml at 50µl/well overnight at 4°C, followed by a 5% BSA blocking step for 2h RT. Human IgG3 (ab138703) was then added starting at 20 µg/ml and plasma/serum at 1:500 and gradually diluted 1:4, 50µl/well for 2h. Ab201248 was then added at 1:10,000 dilution, 50µl/well for 2h. A HRP-streptavidin (ab7403) was used at 1:10,000 dilution for 1h.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (178)
ab7403 被引用在 178 文献中.
- Gasperoni JG et al. The Role of PLAG1 in Mouse Brain Development and Neurogenesis. Mol Neurobiol 61:5851-5867 (2024). PubMed: 38240991
- Griffin TA et al. Fibril treatment changes protein interactions of tau and α-synuclein in human neurons. J Biol Chem 299:102888 (2023). PubMed: 36634849
- Mevissen TET et al. TRIM21-dependent target protein ubiquitination mediates cell-free Trim-Away. Cell Rep 42:112125 (2023). PubMed: 36807144
- Sootichote R et al. Potential Protective Effect of Dengue NS1 Human Monoclonal Antibodies against Dengue and Zika Virus Infections. Biomedicines 11:N/A (2023). PubMed: 36672734
- Agas A et al. Hemolytic iron regulation in traumatic brain injury and alcohol use. Alcohol 109:1-12 (2023). PubMed: 36690222
