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重组Streptolysin O (Hemolytic streptococcus)蛋白(ab63978)

Submit a review Q&A (6)References (5)
SDS-PAGE - Recombinant Streptolysin O (Hemolytic streptococcus) protein (ab63978)

    Key features and details

    • Expression system: Escherichia coli
    • Purity: > 98% SDS-PAGE
    • Active: Yes
    • Tags: His tag N-Terminus
    • Suitable for: SDS-PAGE, Functional Studies

    描述

    • 产品名称

      重组Streptolysin O (Hemolytic streptococcus)蛋白
      参阅全部 Streptolysin O (Hemolytic streptococcus) 蛋白酶

    • 生物活性

      Definition of 1HU is activation of 50% hemolysis by incubating 3% sheep red blood cells at 37? for 30 minutes.

      The specific activity is as high as 1,200,000- 2,000,000 hemolytic units (HU) /mg.

       

    • 纯度

      > 98 % SDS-PAGE.
      Affinity-purified with Ni colum followed by gel-filtration

    • 表达系统

      Escherichia coli

    • Accession

      Q54114

    • 蛋白长度

      Full length protein

    • 无动物成分

      No

    • 性质

      Recombinant

      • 种属

        Streptococcus

      • 序列

        MGGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDPSSR NKQN TANTETTTTN EQPKPESSEL TTEKAGQKMD DMLNSNDMIK LAPKEMPLES AEKEEKKSED NKKSEEDHTE EINDKIYSLN YNELEVLAKN GETIENFVPK EGVKKADKFI VIERKKKNIN TTPVDISIID SVTDRTYPAA LQLANKGFTE NKPDAVVTKR NPQKIHIDLP GMGDKATVEV NDPTYANVST AIDNLVNQWH DNYSGGNTLP ARTQYTESMV YSKSQIEAAL NVNSKILDGT LGIDFKSISK GEKKVMIAAY KQIFYTVSAN LPNNPADVFD KSVTLKELQR KGVSNEAPPL FVSNVAYGRT VFVKLETSSK SNDVEAAFSA ALKGTDVKTN GKYSDILENS SFTAVVLGGD AAEHNKVVTK DFDVIRNVIK DNATFSRKNP AYPISYTSVF LKNNKIAGVN NRSEYVETTS TEYTSGKINL SHQGAYVAQY EILWDEINYD DKGKEVITKR RWDNNWYSKT SPFSTVIPLG ANSRNIRIMA RECTGLAWEW WRKVIDERDV KLSKEINVNI SGSTLSPYGS ITYK

      • 氨基酸

        37 to 574

      • 标签

        His tag N-Terminus

    技术指标

    Our Abpromise guarantee covers the use of ab63978 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • 应用

      SDS-PAGE

      Functional Studies

    • 形式

      Liquid

    • 补充说明

      Information about health hazards:

      Target: Cholesterol on human and animal cell membrane

      Health Hazards: May be fatal if enters bloodstream.

      LD50-Lethal dose (50 percent kill) intravenous,

      Rabbit, 1500 ng/kg (Ref :PHTHDT Pharmacology and Therapeutics. (Pergamon Press Ltd.,Headington Hill Hall, Oxford OX3 0BW, UK) Vol.(Issue) 11, Page 661,1981 )

      Guinea pig, 12 ug/kg (Ref: BICMBE Biochimie. (SPPIF, B.P.22, F-41353 Vineuil, France, Vol.(Issue)55,Page 1187, 1973)

      Toxicity is much less when introduced via other routes of entry like interdermal injection.

    • Concentration information loading...

    制备和贮存

    • 稳定性和存储

      Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.

      pH: 6
      Constituents: 49% Water, 0.015% DTT, 50% Glycerol (glycerin, glycerine), 0.1% Streptolysin, x1 Phosphate Buffered Saline

      This product is an active protein and may elicit a biological response in vivo, handle with caution.

    常规信息

    • 别名

      • Streptolysin O
      • Streptolysin O (Thiol-activated cytolysin) slo

    • 相关性

      Streptolysin O (SLO) is a membrane-damaging extracellular toxin produced by hemolytic streptococci. The membrane-damaging activity is measured by hemolysis of red-blood cell. SLO is oxygen-sensitive and is easily inactivated in its presence but can be reactivated by thiol compounds , so it is also called thiol-activated cytolysin. SLO is produced not only by Group A hemolytic streptococci but also by Group C and Group G strains. The amino acid sequences are highly conserved among them and their homology is over 98%.

    图片

    • SDS-PAGE - Recombinant Streptolysin O (Hemolytic streptococcus) protein (ab63978)
      SDS-PAGE - Recombinant Streptolysin O (Hemolytic streptococcus) protein (ab63978)

      SDS-PAGE analysis of purified Streptolysin O (Hemolytic streptococcus) protein (ab63978).

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    文献 (5)

    发表研究结果有使用 ab63978？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab63978 被引用在 5 文献中.

    • Wang F  et al. Interferon Gamma Induces Reversible Metabolic Reprogramming of M1 Macrophages to Sustain Cell Viability and Pro-Inflammatory Activity. EBioMedicine 30:303-316 (2018). PubMed: 29463472
    • Blanca Ramírez M  et al. GTP binding regulates cellular localization of Parkinson's disease-associated LRRK2. Hum Mol Genet 26:2747-2767 (2017). PubMed: 28453723
    • Siau A  et al. Proteome mapping of Plasmodium: identification of the P. yoelii remodellome. Sci Rep 6:31055 (2016). PubMed: 27503796
    • Lee YK  et al. Modeling of Friedreich ataxia-related iron overloading cardiomyopathy using patient-specific-induced pluripotent stem cells. Pflugers Arch 466:1831-44 (2014). Functional Studies . PubMed: 24327207
    • Gao N & Lehrman MA Mannose-6-phosphate: a regulator of LLO destruction. Methods Mol Biol 1022:277-82 (2013). PubMed: 23765668

    客户评价及客户问答

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    1-6 of 6 Abreviews or Q&A

    Question

    Hi, Re: Inactivation of streptolysin O with formaldehyde. Do you know of a published reference for this inactivation method? Thanks

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    Answer

    Thank you for contacting us.

    I am afraid I am not aware of any publication regarding the inactivation method of Streptolysin O.

    However, it can be done by dialyzing against 0.4% formaldehyde in 100 mM at 30℃ for 3 days.

    I hope this helps. Please do not hesitate to contact us for further assistance.

    Read More

    Question

    What effect the protein will have when injected into sheep?

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    Answer

    Thank you for contacting us.
    Streptolysin O (SLO), prototype of the cholesterol-binding family of bacterial exotoxins, forms very large pores in the plasma membrane of mammalian cells (http://www.ncbi.nlm.nih.gov/pubmed/8593102). I am afraid injection of a large amount of streptolysin will be harmful to animals, so antigen has to be inactivated by dialyzing in 0.4%formaldehyde for 3 days at 30℃. Formalin inactivated toxin is called toxoid. The rest will be the same as ordinary antibody formation with adjuvant.
    If you are interested, please let me know we might provide rabbit antiserum for this protein.
    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Question

    : Do you activate streptolysin O (ab63978) with cysteine before measuring its activity? Also, do you have any data regarding pore formation with this product?

    Read More
    Answer

    Thank you for contacting Abcam.

    Our streptolysin O (SLO) is active without pretreatment probably because the solution contains 1 mM DTT. We measured pore-forming activity by hemolysin activity.

    If there is anything else I can help you with, please let me know.

    Read More

    Question

    Do you have an extinction coefficient for product number ab63978, Streptolysin O protein?

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    Answer

    Thank you for contacting Abcam.
    To find the extinction coefficient, I used the follwing link:
    http://encorbio.com/protocols/Prot-MW-Abs.htm
    For Streptolysin O protein ;
    There are 571 Amino acids and Molecular Weight is 63.64 KiloDaltons
    Charge is 3.5 assuming N and C termini are not blocked
    There are 22 Tyrosines and 7 Tryptophans
    The Molar Extinction Coefficient is 71280
    The absorbance of a 1mg/ml solution at 280nm is 1.120
    Please let me know if there is anything else I can help you with.

    Read More

    Question

    Do you have any additional details about the protocol that is used to measure the activity of this protein?

    Read More
    Answer

    Thank you for contacting us.

    The activity of this Streptolysin O proteinis measured by applying the protein to 3% sheep red blood cells in PBS, pH 7.4 at 37C for 30 minutes and counting the lysed cells under the microscope. Using this protocol,1HU is defined as the amount of protein required to produce 50% hemolysis.

    I hope this helps, please let me know if you need any additional information.

    Read More

    Question

    Is this protein produced under GMP conditions?

    Read More
    Answer

    This protein is not produced under GMP conditions. It is not for human use and is for research use only. I hope this is helpful. Please contact me again if you need any further information.

    Read More

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