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AB52953

Recombinant Staphylococcus aureus Protein A

Recombinant Staphylococcus aureus Protein A

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(3 Publications)

Recombinant Staphylococcus aureus Protein A is a Fragment protein in the 32 to 327 aa range with >99% purity, < 0.1 EU/mg endotoxin level and suitable for SDS-PAGE.The predicted molecular weight of ab52953 protein is 35.5 kDa.

- Save time and ensure accurate results - use our recombinant S. aureus Protein A (SpA) to probe and detect IgG

查看别名

SAOUHSC_00069, spa, Immunoglobulin G-binding protein A, IgG-binding protein A, Staphylococcal protein A, SpA

2 Images
Size Exclusion Chromatography - Recombinant Staphylococcus aureus Protein A (AB52953)
  • Size Exclusion Chromatography

Supplier Data

Size Exclusion Chromatography - Recombinant Staphylococcus aureus Protein A (AB52953)

SEC chromatography of Protein A. The apparent molecular weight is ~ 70 KDa calculated based on SEC standard curve.

SDS-PAGE - Recombinant Staphylococcus aureus Protein A (AB52953)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Staphylococcus aureus Protein A (AB52953)

SDS-PAGE (4-20%) of Recombinant Protein A.

Lane 1-3 : 5, 2.5, and 1 μg, Protein A under Reducing conditions,

Lane4 : protein Ladder;

Lane5-7 : 5, 2.5, and 1 μg of Protein A under Non-Reducing conditions

关键信息

纯度

>99% SDS-PAGE

内毒素水平

< 0.1 EU/mg

表达系统

Escherichia coli

标签

6x His tag N-Terminus

应用

SDS-PAGE

applications

生物活性

No

访问

P02976

不含动物源

Yes

不含载体蛋白

No

种属

Staphylococcus aureus subsp. aureus NCTC 8325

存储溶液

Constituents: Water

storage-buffer

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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产品详情

Ensure the validity of your result using our recombinant S. aureus Protein A ab52953 to probe and detect IgG in SDS-PAGE.

Check out our protein gel staining guide for SDS-PAGE here


Function

The cell wall binding region, albumin binding region and other non-specific binding regions have been eliminated from this protein to ensure the maximum specific IgG binding. The 6x His-tag can be used for affinity purification or for Protein A detection using anti-His-tag antibody. The recombinant Protein A is ideal for purification of polyclonal or monoclonal IgG antibodies.

Protein A binds to most human and mouse IgG subclasses (e.g: human IgG1, IgG2, IgG4; mouse IgG1, IgG2a, IgG2b, IgG3).

It also binds to total IgG from cow, guinea pig, hamster, horse, pig, and rabbit.
Protein A has little affinity to chicken, goat, rat and sheep.


This product is manufactured by BioVision, an Abcam company and was previously called 6500 Protein A (Liquid form). 6500-10 is the same size as the 10 mg size of ab52953.
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序列信息

[{"sequence":"AANAAQHDEAQQNAFYQVLNMPNLNADQRNGFIQSLKDDPSQSANVLGEAQKLNDSQAPKADAQQNNFNKDQQSAFYEILNMPNLNEAQRNGFIQSLKDDPSQSTNVLGEAKKLNESQAPKADNNFNKEQQNAFYEILNMPNLNEEQRNGFIQSLKDDPSQSANLLSEAKKLNESQAPKADNKFNKEQQNAFYEILHLPNLNEEQRNGFIQSLKDDPSQSANLLAEAKKLNDAQAPKADNKFNKEQQNAFYEILHLPNLTEEQRNGFIQSLKDDPSVSKEILAEAKKLNDAQAPK","proteinLength":"Fragment","predictedMolecularWeight":"35.5 kDa","actualMolecularWeight":null,"aminoAcidEnd":327,"aminoAcidStart":32,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"P02976","tags":[{"tag":"6x His","terminus":"N-Terminus"}]}]
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性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
-20°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
False
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特殊说明

形式

Liquid

附加说明

Purity: >99% by SDS-PAGE and HPLC analyses.

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常规信息

功能

Plays a role in the inhibition of the host innate and adaptive immune responses. Possesses five immunoglobulin-binding domains that capture both the fragment crystallizable region (Fc region) and the Fab region (part of Ig that identifies antigen) of immunoglobulins (PubMed : 10805799, PubMed : 2938951, PubMed : 4163007). In turn, Staphylococcus aureus is protected from phagocytic killing via inhibition of Ig Fc region. In addition, the host elicited B-cell response is prevented due to a decrease of antibody-secreting cell proliferation that enter the bone marrow, thereby decreasing long-term antibody production. Inhibits osteogenesis by preventing osteoblast proliferation and expression of alkaline phosphatase, type I collagen, osteopontin and osteocalcin. Acts directly as a pro-inflammatory factor in the lung through its ability to bind and activate tumor necrosis factor alpha receptor 1/TNFRSF1A (By similarity).

序列相似性

Belongs to the immunoglobulin-binding protein SpA family.

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产品实验方案

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靶点信息

Plays a role in the inhibition of the host innate and adaptive immune responses. Possesses five immunoglobulin-binding domains that capture both the fragment crystallizable region (Fc region) and the Fab region (part of Ig that identifies antigen) of immunoglobulins (PubMed : 10805799, PubMed : 2938951, PubMed : 4163007). In turn, Staphylococcus aureus is protected from phagocytic killing via inhibition of Ig Fc region. In addition, the host elicited B-cell response is prevented due to a decrease of antibody-secreting cell proliferation that enter the bone marrow, thereby decreasing long-term antibody production. Inhibits osteogenesis by preventing osteoblast proliferation and expression of alkaline phosphatase, type I collagen, osteopontin and osteocalcin. Acts directly as a pro-inflammatory factor in the lung through its ability to bind and activate tumor necrosis factor alpha receptor 1/TNFRSF1A (By similarity).
See full target information spa
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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

Diagnostics (Basel, Switzerland) 13: PubMed37175021

2023

Application of a Novel One-Side Cell Quartz Crystal Microbalance Immunosensor in the Determination of Alpha-Fetoprotein from Human Serum.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Chen,Huashan Shi,Bo Mu

The Analyst 144:5108-5116 PubMed31373337

2019

Influence of antibody immobilization strategy on carbon electrode immunoarrays.

Applications

Unspecified application

Species

Unspecified reactive species

Mohamed Sharafeldin,Kira McCaffrey,James F Rusling

Small (Weinheim an der Bergstrasse, Germany) 14:e1802865 PubMed30334351

2018

Microparticle Delivery of Protein Markers for Single-Cell Western Blotting from Microwells.

Applications

Unspecified application

Species

Unspecified reactive species

John J Kim,Peggy P Y Chan,Julea Vlassakis,Alisha Geldert,Amy E Herr
View all publications
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搭配使用产品

Reagents, Controls & Accessories

AB325996

Prestained Protein Ladder - Broad molecular weight (11-250 kDa, 5-color, 12 bands)

SDS-PAGE - Prestained Protein Ladder - Broad molecular weight (11-250 kDa, 5-color, 12 bands) (AB325996)

  • 0
  • 0
Proteins & Peptides

AB52213

Recombinant Protein A + Protein G

SDS-PAGE - Recombinant Protein A + Protein G (AB52213)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB18596

HRP Anti-Protein A antibody

  • 0
  • 0
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88

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