重组SARS spike glycoprotein (Coronavirus) (ab49046)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Tags: His tag C-Terminus
- Suitable for: ELISA, WB
描述
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产品名称
重组SARS spike glycoprotein (Coronavirus)
参阅全部 SARS spike glycoprotein 蛋白酶 -
纯度
> 90 % SDS-PAGE.
Purified by affinity chromatography. -
表达系统
Escherichia coli -
蛋白长度
Protein fragment -
无动物成分
No -
性质
Recombinant -
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序列
RVDFCGKGYH LMSFPQAAPH GVVFLHVTYV PSQERNFTTA PAICHEGKAY FPREGVFVFN GTSWFITQRN FFSPQIITTD NTFVSGNCDV VIGIINNTVY DPLQPELDSF KEELDKYFKN HTSPDVDLGD ISGINASVVN IQKEIDRLNE VAKNLNESLI DLQELGKYEQ YIKWPWYVWL GFIAGLIAIV MVTILLCCMT SCCSCLKGAC SCGSCCKFDE DDSEPVLKGV KLHYT -
预测分子量
27 kDa -
标签
His tag C-Terminus -
额外的序列信息
E. coli recombinant containing Spike protein immunodominant regions Fusions Six histidines. This protein is from the Spike protein S1 region.
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技术指标
Our Abpromise guarantee covers the use of ab49046 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
ELISA
Western blot
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形式
Liquid -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
pH: 7.20
Constituents: 9% Urea, 0.32% Tris HCl, 50% Glycerol (glycerin, glycerine)
常规信息
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别名
- E2
- E2 glycoprotein
- Human coronavirus spike glycoprotein
see all -
相关性
A novel coronavirus has been identified as the causative agent of SARS (Severe Acute Respiratory Syndrome). Coronaviruses are a major cause of upper respiratory diseases in humans. The genomes of these viruses are positive stranded RNA approximately 27 to 31kb in length. SARS infection can be mediated by the binding of the viral spike protein, a glycosylated 139 kDa protein and the major surface antigen of the virus, to the angiotensin converting enzyme 2 (ACE2) on target cells. This binding can be blocked by a soluble form of ACE2.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab49046 被引用在 3 文献中.
- Desai AV et al. Surface-Functionalized Metal-Organic Frameworks for Binding Coronavirus Proteins. ACS Appl Mater Interfaces 15:9058-65 (2023). PubMed: 36786318
- Gasparello J et al. Sulforaphane inhibits the expression of interleukin-6 and interleukin-8 induced in bronchial epithelial IB3-1 cells by exposure to the SARS-CoV-2 Spike protein. Phytomedicine 87:153583 (2021). PubMed: 34033999
- Gasparello J et al. In vitro induction of interleukin-8 by SARS-CoV-2 Spike protein is inhibited in bronchial epithelial IB3-1 cells by a miR-93-5p agomiR. Int Immunopharmacol 101:108201 (2021). PubMed: 34653729