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重组S. cerevisiae Glutaredoxin 1蛋白(ab63231)

Submit a review Q&A (1)References (2)

Key features and details

  • Expression system: Escherichia coli
  • Purity: > 90% SDS-PAGE
  • Active: Yes
  • Tags: His tag C-Terminus
  • Suitable for: Functional Studies

描述

  • 产品名称

    重组S. cerevisiae Glutaredoxin 1蛋白

  • 生物活性

     

    Strongly binds to Glutathione, reduced and oxidized.

  • 纯度

    > 90 % SDS-PAGE.

  • 表达系统

    Escherichia coli

  • Accession

    850322

  • 蛋白长度

    Full length protein

  • 无动物成分

    No

  • 性质

    Recombinant

    • 种属

      Saccharomyces cerevisiae

    • 标签

      His tag C-Terminus

  • 描述

    重组S. cerevisiae Glutaredoxin 1蛋白

技术指标

Our Abpromise guarantee covers the use of ab63231 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    Functional Studies

  • 形式

    Liquid
  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituent: 0.395% Tris HCl

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (2)

发表研究结果有使用 ab63231？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab63231 被引用在 2 文献中.

  • Yao F  et al. Low frequency pulsed electromagnetic field promotes differentiation of oligodendrocyte precursor cells through upregulation of miR-219-5p in vitro. Life Sci 223:185-193 (2019). PubMed: 30885522
  • Park HA  et al. Glutathione disulfide induces neural cell death via a 12-lipoxygenase pathway. Cell Death Differ 16:1167-79 (2009). PubMed: 19373248

客户评价及客户问答

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Question

Is this recombinant human glutaredoxin 1? What is the activity of the enzyme (units/ml)?

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Answer

The Glutaredoxin 1 protein (Active), ab63231, is the Glutaredoxin 1 protein from Saccharomyces cerevisiae (SwissProt reference P25373).
The activity of the protein was ascertained by monitoring its ability to oxidize NADPH. The activity was calculated to be 3X10^-3 micromol/min. The reaction mix contained NADPH (0.4 mM), GSH (1.0 mM), glutathione reductase (17 ug) and HED (1.4 mM) in 0.1 M Tris HCl, pH 7.4.
A mixed disulphide between HED and GSH is formed within 2 minutes, and the reaction was started by the addition of 17 ug Grx1. The reaction was followed by the decrease in A340 due to the oxidation of NADPH.

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