重组RFP蛋白(ab51993)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE, WB, Flow Cyt
描述
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产品名称
重组RFP蛋白
参阅全部 RFP 蛋白酶 -
纯度
> 95 % SDS-PAGE.
The recombinant RFP (Red Fluorescence Protein) is expressed and purified from transformed E. coli using a method that ensures high purity and maximal fluorescence of the protein.
Endotoxin was removed for in vivo injection or cell culture applications. Endotoxin level equal to or less than 0.1 ng/µg. -
表达系统
Escherichia coli -
蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Discosoma -
预测分子量
28 kDa including tags -
标签
His tag N-Terminus
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相关产品
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Related Products
技术指标
Our Abpromise guarantee covers the use of ab51993 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
Western blot
Flow Cytometry
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形式
Lyophilized -
补充说明
This product is manufactured by BioVision, an Abcam company and was previously called 4997 dsRed Fluorescent Protein. 4997-100 is the same size as the 100 µg size of ab51993.
This protein (DSRed) is suitable as control reagent for RFP expression studies or as a labeling reagent.
Endotoxin level is <0.1 ng/μg.
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
pH: 7.40
Constituents: 10.269% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Monobasic dihydrogen potassium phosphate
常规信息
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别名
- DsRed
- GFP like chromoprotein
- red fluorescent protein
- RFP
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相关性
Fluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions.
图片
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SDS-PAGE - Recombinant RFP protein (ab51993)
ab51993 loaded under reducing conditions and stained with coomassive blue.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab51993 被引用在 1 文献中.
- Dufour YS et al. Direct Correlation between Motile Behavior and Protein Abundance in Single Cells. PLoS Comput Biol 12:e1005041 (2016). PubMed: 27599206