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AB92846

重组大鼠VEGFA蛋白

Recombinant rat VEGFA protein

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(0 Publication)

Recombinant rat VEGFA protein is a Rat Fragment protein, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, FuncS.

查看别名

Vegf, Vegfa, Vascular endothelial growth factor A, VEGF-A, Vascular permeability factor, VPF

3 Images
Functional Studies - Recombinant rat VEGFA protein (AB92846)
  • FuncS

Unknown

Functional Studies - Recombinant rat VEGFA protein (AB92846)

Functional analysis of ab92846

Functional Studies - Recombinant rat VEGFA protein (AB92846)
  • FuncS

Unknown

Functional Studies - Recombinant rat VEGFA protein (AB92846)

ab92846 used in Functional Studies.

SDS-PAGE - Recombinant rat VEGFA protein (AB92846)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant rat VEGFA protein (AB92846)

SDS PAGE analysis of ab92846 under non-reducing (-) and reducing (+) conditions. Stained with Coomassie Blue.

关键信息

纯度

>95% SDS-PAGE

表达系统

Escherichia coli

标签

Tag free

应用

SDS-PAGE, FuncS

applications

生物活性

Yes

生物学活性

Biological Activity: As determined by the dose-dependent induced proliferation of Human umbilical vein endothelial cells (HUVECs) when compared to a standard. The expected ED50 is 4.0-8.0 ng/ml.

访问

P16612

不含动物源

No

不含载体蛋白

No

种属

Rat

复溶

Reconstitute at 0.1 mg/mL in water

存储溶液

Constituents: 0.165% Sodium phosphate

storage-buffer

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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产品详情

Endotoxin Level:Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.
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序列信息

[{"sequence":"MAPTTEGEQKAHEVVKFMDVYQRSYCRPIETLVDIFQEYPDEIEYIFKPSCVPLMRCAGCCNDALECVPTSESNVTMQIMRIKPHQSQHIGEMSFLQHSRCECRPKKDRTKPEKHCEPCSERRKHLFVQDPQTCKCSCKNTDSRCKARQLELNERTCRCDKPRR","proteinLength":"Fragment","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P16612","tags":[]}]
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性能和储存信息

运输条件
Blue Ice
推荐的短期储存条件
-20°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
True
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Vascular endothelial growth factor A (VEGFA) also known as VEGF or vascular permeability factor (VPF) is a protein that plays a central role in angiogenesis as well as in the growth of blood vessels. The molecular weight of VEGFA varies depending on the isoform typically ranging from 34 to 42 kDa. VEGFA is expressed in many tissues including fibroblasts macrophages endothelial cells and platelets. Its expression levels can increase in response to stimuli like hypoxia or inflammatory cytokines. VEGFA is an important target in research for therapies related to vascular diseases and cancer.
Biological function summary

VEGFA functions by promoting the proliferation and migration of vascular endothelial cells. It operates both as a homodimer and as part of complex signaling cascades interacting with VEGF receptors on cell surfaces to trigger downstream signals for angiogenesis. This includes endothelial cell growth migration and the new blood vessel formation process. VEGFA's activity is important for physiological processes such as wound healing and embryonic development and it also contributes to pathological conditions through its unregulated expression in diseases.

Pathways

Many processes involve VEGFA including the PI3K/AKT pathway and the MAPK/ERK pathway. These pathways regulate cell survival growth and differentiation. Other proteins like PLGF (placenta growth factor) and VEGFB often accompany VEGFA in these pathways aiding in distinct but overlapping roles. These interactions are transmitted through binding to VEGF receptors such as VEGFR-1 and VEGFR-2 subsequently activating various signaling cascades necessary for cellular and tissue homeostasis.

VEGFA relates to cancer and age-related macular degeneration among others. In cancer overexpression of VEGFA can lead to excessive angiogenesis supplying blood to tumors and facilitating tumor growth. In age-related macular degeneration upregulation of VEGFA contributes to the progression of the disease by promoting the formation of abnormal blood vessels under the retina. Anti-VEGFA therapies such as anti-VEGF antibodies (e.g. bevacizumab) can target these overexpressed pathways. VEGFA interacts with other proteins like HIF-1α which regulates its expression under hypoxia a common condition in tumors.
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特殊说明

形式

Lyophilized

附加说明

Purity is approximately 97% as determined by RP-HPLC and reducing and non-reducing SDS-PAGE.

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常规信息

功能

Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. May play a role in increasing vascular permeability during lactation, when increased transport of molecules from the blood is required for efficient milk protein synthesis. Binding to NRP1 receptor initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development (By similarity). Also binds the DEAR/FBXW7-AS1 receptor (By similarity).

序列相似性

Belongs to the PDGF/VEGF growth factor family.

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产品实验方案

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靶点信息

Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. May play a role in increasing vascular permeability during lactation, when increased transport of molecules from the blood is required for efficient milk protein synthesis. Binding to NRP1 receptor initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development (By similarity). Also binds the DEAR/FBXW7-AS1 receptor (By similarity).
See full target information Vegfa
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搭配使用产品

Reagents, Controls & Accessories

AB325994

Prestained Protein Ladder - Mid-range molecular weight (11-180 kDa, 4-color)

SDS-PAGE - Prestained Protein Ladder - Mid-range molecular weight (11-180 kDa, 4-color) (AB325994)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB46154

Anti-VEGFA antibody

Lab Essentials

Western blot - Anti-VEGFA antibody (AB46154)

  • 5
  • 29
Primary Antibodies

AB7817

Anti-alpha smooth muscle Actin antibody [1A4]

KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [1A4] (AB7817)

  • 5
  • 105
Assay Kits

AB222510

Human VEGF ELISA Kit

SimpleStep

ELISA - Human VEGF ELISA Kit (AB222510)

  • 5
  • 1

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