重组大鼠Thrombopoietin蛋白(ab129140)
Key features and details
- Expression system: Escherichia coli
- Purity: > 98% SDS-PAGE
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE, HPLC
描述
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产品名称
重组大鼠Thrombopoietin蛋白
参阅全部 Thrombopoietin 蛋白酶 -
生物活性
Determined by its ability to stimulate the proliferation of Human MO7e cells. The expected ED50 is = 0.2 ng/ml, corresponding to a specific activity of = 5 x 106 units/mg.
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纯度
> 98 % SDS-PAGE.
Purity: > 98% by SDS-PAGE and HPLC analysis. -
表达系统
Escherichia coli -
Accession
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蛋白长度
Protein fragment -
无动物成分
No -
性质
Recombinant -
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种属
Rat -
序列
SPVPPACDPR LLNKLLRDSY LLHSRLSQCP DVNPLSIPVL LPAVDFSLGE WKTQTEQSKA QDILGAVSLL LEGVMAARGQ LEPSCLSSLL GQLSGQVRLL LGALQGLLGT QLPPQGRTTA HKDPSALFLS LQQLLRGKVR FLLLVEGPAL CVRRTLPTTA VPSRTSQLLT LNKF -
预测分子量
19 kDa
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技术指标
Our Abpromise guarantee covers the use of ab129140 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Functional Studies
SDS-PAGE
HPLC
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形式
Lyophilized -
补充说明
Endotoxin level: < 0.1 ng/µg of Thrombopoietin protein.
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. The lyophilized protein is stable for a few weeks at room temperature. Store at -20°C long term.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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复溶Reconstituted ab129140 should be stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles.
常规信息
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别名
- C mpl ligand
- C-mpl ligand
- Megakaryocyte colony stimulating factor
see all -
功能
Lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from their committed progenitor cells. It acts at a late stage of megakaryocyte development. It may be the major physiological regulator of circulating platelets. -
疾病相关
Defects in THPO are a cause of essential thrombocythemia (ET) [MIM:187950]. ET is inherited as an autosomal dominant trait which is characterized by elevated platelet levels due to sustained proliferation of megakaryocytes, and frequently lead to thrombotic and haemorrhagic complications. -
序列相似性
Belongs to the EPO/TPO family. -
结构域
Two-domain structure with an erythropoietin-like N-terminal and a Ser/Pro/Thr-rich C-terminal. -
细胞定位
Secreted. - Information by UniProt
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (0)
ab129140 尚未被引用在任何文献中。