Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: Inhibition Assay
参阅全部 PTP1B 蛋白酶
The specific activity of rat PTP1B was determined using pNPP. Enzyme reaction condition: 20 mM pNPP, 5 min incubation at 30C, 2 ug/ml enzyme.
纯度> 95 % SDS-PAGE.
蛋白长度Full length protein
氨基酸1 to 321
标签GST tag N-Terminus
Our Abpromise guarantee covers the use of ab42573 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 0.307% Glutathione, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.395% Tris HCl, 0.05% Tween, 50% Glycerol (glycerin, glycerine), 0.435% Sodium chloride
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- Non receptor tyrosine phosphatase 1
- Protein phosphotyrosylphosphatase 1B
功能May play an important role in CKII- and p60c-src-induced signal transduction cascades.
序列相似性Belongs to the protein-tyrosine phosphatase family. Non-receptor class 1 subfamily.
Contains 1 tyrosine-protein phosphatase domain.
翻译后修饰Oxidized on Cys-215; the Cys-SOH formed in response to redox signaling reacts with the alpha-amido of the following residue to form a 4-amino-3-isothiazolidinone serine cross-link, triggering a conformational change that inhibits substrate binding and activity. The active site can be restored by reduction.
细胞定位Endoplasmic reticulum membrane.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.