重组大鼠IL-7蛋白(Active) (ab290092)
Key features and details
- Expression system: HEK 293 cells
- Purity: >= 95% HPLC
- Endotoxin level: <= 0.005 Eu/µg
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE, HPLC, MS
描述
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产品名称
重组大鼠IL-7蛋白(Active)
参阅全部 IL-7 蛋白酶 -
生物活性
Fully biologically active determined by the dose dependent proliferation of 2E8 cells.
ED50 is ≤ 16.3 ng/ml, corresponding to a specific activity of 6.1 x 104 units/mg.
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纯度
>= 95 % HPLC.
SDS-PAGE >=95% -
内毒素水平
<=0.005 Eu/µg -
表达系统
HEK 293 cells -
Accession
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蛋白长度
Full length protein -
无动物成分
Yes -
无载体
是 -
性质
Recombinant -
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种属
Rat -
序列
DCHIKDKDGKAFGSVLMISINQLDKMTGTDSDCPNNEPNFFKKHLCDDTK EAAFLNRAARKLRQFLKMNISEEFNDHLLRVSDGTQTLVNCTSKEEKTIK EQKKNDPCFLKRLLREIKTCWNKILKGSI -
预测分子量
15 kDa -
实际分子量
15 kDa -
分子量信息
Predicted MW is 14936.21 Da (+/-10 Da by ESI-TOF). Observed MW is 14938.09 Da. -
氨基酸
26 to 154 -
额外的序列信息
N-terminal glycine
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描述
重组大鼠IL-7蛋白
技术指标
Our Abpromise guarantee covers the use of ab290092 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Functional Studies
SDS-PAGE
HPLC
Mass Spectrometry
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形式
Lyophilized -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at Room Temperature. Store at Room Temperature.
pH: 7.4
Constituents: 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Monobasic dihydrogen potassium phosphate, 10.26% TrehaloseThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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复溶Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product. Store lyophilized form at room temperature. Reconstitute in phosphate buffered saline, aliquot and store at -80°C for 12 months or +4°C for 1 week. Avoid repeated freeze-thaw.
常规信息
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别名
- IL 7
- IL-7
- Il7
see all -
功能
Hematopoietic growth factor capable of stimulating the proliferation of lymphoid progenitors. It is important for proliferation during certain stages of B-cell maturation. -
序列相似性
Belongs to the IL-7/IL-9 family. -
细胞定位
Secreted. - Information by UniProt
图片
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Functional Studies - Recombinant Rat IL-7 Protein (ab290092)
Fully biologically active determined by the dose dependent proliferation of 2E8 cells.
ED50 is ≤ 16.3 ng/ml, corresponding to a specific activity of 6.1 x 104 units/mg.
Cell based assay testing is performed on the first lot of protein only and is provided as a reference for protein activity; subsequent lots of protein must pass all biophysical quality control parameters that meet the same parameters as the first lot. Lot GR3453026-1.
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Mass Spectrometry - Recombinant Rat IL-7 Protein (ab290092)Mass determination by ESI-TOF. Predicted MW is 14936.21 Da (+/-10 Da by ESI-TOF). Observed MW is 14938.09 Da.
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HPLC - Recombinant Rat IL-7 Protein (ab290092)HPLC analysis of ab290092
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SDS-PAGE - Recombinant Rat IL-7 Protein (ab290092)SDS-PAGE analysis of ab290092
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (0)
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