重组Raf1 (mutated Y340E + Y341E)蛋白(Active) (ab62292)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 80% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: WB, Functional Studies
描述
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产品名称
重组Raf1 (mutated Y340E + Y341E)蛋白(Active)
参阅全部 Raf1 蛋白酶 -
生物活性
The specific activity of RAF1 was determined to be ~6,000 nmol/min/mg in a coupled assay as per antibody assay protocol.
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纯度
> 80 % SDS-PAGE.
Purity >80% as determined by SDS-PAGE and Coomassie blue staining. Affinity purified. -
表达系统
Baculovirus infected Sf9 cells -
Accession
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蛋白长度
Protein fragment -
无动物成分
No -
性质
Recombinant -
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预测分子量
63 kDa including tags -
氨基酸
306 to 648 -
修饰
mutated Y340E + Y341E -
标签
GST tag N-Terminus
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技术指标
Our Abpromise guarantee covers the use of ab62292 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Western blot
Functional Studies
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形式
Liquid -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
pH: 7.50
Constituents: 0.00385% DTT, 0.79% Tris HCl, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.31% Glutathione, 0.0029% EDTA, 0.0017% PMSFThis product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
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别名
- c Raf
- C-raf
- C-Raf proto-oncogene, serine/threonine kinase
see all -
功能
Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. Part of the Ras-dependent signaling pathway from receptors to the nucleus. Protects cells from apoptosis mediated by STK3. -
组织特异性
In skeletal muscle, isoform 1 is more abundant than isoform 2. -
疾病相关
Defects in RAF1 are the cause of Noonan syndrome type 5 (NS5) [MIM:611553]. Noonan syndrome (NS) is a disorder characterized by dysmorphic facial features, short stature, hypertelorism, cardiac anomalies, deafness, motor delay, and a bleeding diathesis. It is a genetically heterogeneous and relatively common syndrome, with an estimated incidence of 1 in 1000-2500 live births.
Defects in RAF1 are the cause of LEOPARD syndrome type 2 (LEOPARD2) [MIM:611554]. LEOPARD syndrome is an autosomal dominant disorder allelic with Noonan syndrome. The acronym LEOPARD stands for lentigines, electrocardiographic conduction abnormalities, ocular hypertelorism, pulmonic stenosis, abnormalities of genitalia, retardation of growth, and deafness. -
序列相似性
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 protein kinase domain.
Contains 1 RBD (Ras-binding) domain. -
翻译后修饰
Phosphorylated upon DNA damage, probably by ATM or ATR. Phosphorylation at Thr-269 increases its kinase activity. Phosphorylation at Ser-259 induces the interaction with YWHAZ and inactivates kinase activity. Dephosphorylation of Ser-259 by the complex containing protein phosphatase 1, SHOC2 and M-Ras/MRAS relieves inactivation, leading to stimulate RAF1 activity. -
细胞定位
Cytoplasm. Cell membrane. Colocalizes with RGS14 and BRAF in both the cytoplasm and membranes. - Information by UniProt
图片
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Functional Studies - Recombinant Raf1 (mutated Y340E + Y341E) protein (Active) (ab62292)
ab62292 Kinase assay plot
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Western blot - Recombinant Raf1 (mutated Y340E + Y341E) protein (Active) (ab62292)Recombinant Raf1 (mutated Y340E + Y341E) protein (Active) (ab62292) + RAF1
Predicted band size: 63 kDa
Observed band size: 63 kDa
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab62292 尚未被引用在任何文献中。