重组兔IL-2蛋白(Active) (ab307411)
Key features and details
- Expression system: HEK 293 cells
- Purity: >= 95% HPLC
- Endotoxin level: <= 0.005 Eu/µg
- Active: Yes
- Suitable for: HPLC, SDS-PAGE, MS
描述
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产品名称
重组兔IL-2蛋白(Active)
参阅全部 IL-2 蛋白酶 -
生物活性
Fully biologically active determined by the dose dependent proliferation of CTLL-2 cells. ED50 is ≤ 1.35 ng/ml, corresponding to a specific activity of 7.4 x 105 units/mg.
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纯度
>= 95 % HPLC.
SDS-PAGE >= 95% -
内毒素水平
<=0.005 Eu/µg -
表达系统
HEK 293 cells -
Accession
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蛋白长度
Full length protein -
无动物成分
Yes -
无载体
是 -
性质
Recombinant -
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种属
Rabbit -
序列
APTSSSTKETQEQLDQLLLDLQVLLKGVNDYKNSKLSRMLTFKFYMPKKV TELKHLQCLEEELKPLEEVLNLAQGKNSHGGNTRESISNINVTVLKLKGS ETFMCEYDETVTIVEFLNRWITFCQSIISASSS -
预测分子量
15 kDa -
实际分子量
15 kDa -
分子量信息
Predicted MW is 15162.36 Da (+/- 10 Da by ESI-TOF). Observed MW is 15162.91 Da. -
氨基酸
21 to 153 -
额外的序列信息
N-terminal glycine (On N-Term)
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技术指标
Our Abpromise guarantee covers the use of ab307411 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
HPLC
SDS-PAGE
Mass Spectrometry
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形式
Lyophilized -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at Room Temperature. Store at Room Temperature.
pH: 7.40
Constituents: 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Monobasic dihydrogen potassium phosphate, 10.26% TrehaloseThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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复溶Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product. Store lyophilized form at room temperature. Reconstitute in phosphate buffered saline, aliquot and store at -80°C for 12 months or +4°C for 1 week. Avoid repeated freeze-thaw.
常规信息
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别名
- Aldesleukin
- IL 2
- IL-2
see all -
功能
Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells. -
疾病相关
Note=A chromosomal aberration involving IL2 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with involves TNFRSF17. -
序列相似性
Belongs to the IL-2 family. -
细胞定位
Secreted. - Information by UniProt
图片
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Biological Activity - Recombinant Rabbit IL-2 Protein (Active) (ab307411)
Fully biologically active determined by the dose dependent proliferation of CTLL-2 cells. ED50 is ≤ 1.35 ng/ml, corresponding to a specific activity of 7.4 x 105 units/mg.
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Mass Spectrometry - Recombinant Rabbit IL-2 Protein (ab307411)Mass determination by ESI-TOF. Predicted MW is 15162.36 Da (+/-10 Da by ESI-TOF). Observed MW is 15162.91 Da.
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HPLC - Recombinant Rabbit IL-2 Protein (ab307411)HPLC analysis of ab307411
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SDS-PAGE - Recombinant Rabbit IL-2 Protein (ab307411)SDS-PAGE analysis of ab307411
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (0)
ab307411 尚未被引用在任何文献中。
