重组Proteinase K (Active) (ab281339)
Key features and details
- Expression system: Yeast
- Purity: >= 95% SDS-PAGE
- Endotoxin level: < 0.250 Eu/mg
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
描述
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产品名称
重组Proteinase K (Active)
参阅全部 Proteinase K 蛋白酶 -
生物活性
Specific activity >30 units/mg.
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纯度
>= 95 % SDS-PAGE. -
内毒素水平
< 0.250 Eu/mg -
表达系统
Yeast -
Accession
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蛋白长度
Full length protein -
无动物成分
Yes -
性质
Recombinant -
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序列
AAQTNAPWGL ARISSTSPGT STYYYDESAG QGSCVYVIDT GIEASHPEFE GRAQMVKTYY YSSRDGNGHG THCAGTVGSR TYGVAKKTQL FGVKVLDDNG SGQYSTIIAG MDFVASDKNN RNCPKGVVAS LSLGGGYSSS VNSAAARLQS SGVMVAVAAG NNNADARNYS PASEPSVCTV GASDRYDRRS SFSNYGSVLD IFGPGTSILS TWIGGSTRSI SGTSMATPHV AGLAAYLMTL GKTTAASACR YIADTANKGD LSNIPFGTVN LLAYNNYQA -
预测分子量
29 kDa including tags -
氨基酸
106 to 384 -
标签
His tag N-Terminus -
额外的序列信息
Tritirachium album (Engyodontium album). Full-length mature chain lack the signal and pro-peptides.
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技术指标
Our Abpromise guarantee covers the use of ab281339 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
Functional Studies
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形式
Lyophilized -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Store at -20°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 6% Trehalose, 0.32% Tris HCl
Buffer lyophilized from.This product is an active protein and may elicit a biological response in vivo, handle with caution.
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复溶We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%.
常规信息
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别名
- Endopeptidase K
- Tritirachium alkaline proteinase
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相关性
Proteinase K is an endolytic protease that cleaves peptide bonds preferentially next to the carboxyl group of N-substituted hydrophobic aliphatic and aromatic amino acids. Proteinase K is classified as a serine protease and exhibits broad substrate specificity. It can hydrolyze keratin; hence the name. Proteinase K is used for the digestion of proteins on tissue sections as a pre-treatment to aid in the preparation of mRNA and its detection by in situ hybridisation methods using oligonucleotide probes.
图片
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SDS-PAGE - Recombinant Proteinase K (Active) (ab281339)
SDS-PAGE analysis of ab281339. 15% gel. Reducing conditions.
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Functional Studies - Recombinant Proteinase K (Active) (ab281339)Specific activity >30 Units/mg.
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Functional Studies - Recombinant Proteinase K (Active) (ab281339)Agarose gel analysis of residual nucleic acid in ab281339.
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Functional Studies - Recombinant Proteinase K (Active) (ab281339)Agarose gel analysis of RNAase activity in ab281339.
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Functional Studies - Recombinant Proteinase K (Active) (ab281339)Agarose gel analysis of DNAase activity in ab281339.
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Functional Studies - Recombinant Proteinase K (Active) (ab281339)Functional analysis of ab281339.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (1)
ab281339 被引用在 1 文献中.
- He JY et al. YY1-induced lncRNA XIST inhibits cartilage differentiation of BMSCs by binding with TAF15 to stabilizing FUT1 expression. Regen Ther 20:41-50 (2022). PubMed: 35402663
