重组蛋白G (HRP) (ab7460)
Key features and details
- Expression system: Escherichia coli
- Purity: > 98% n/a
- Suitable for: WB, Dot blot, ELISA, IHC-P
描述
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产品名称
重组蛋白G (HRP)
参阅全部 Protein G 蛋白酶 -
纯度
> 98 % n/a.
Protein G Peroxidase was prepared from chromatographically pure recombinant Protein G. -
表达系统
Escherichia coli -
Accession
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蛋白长度
Protein fragment -
无动物成分
No -
性质
Recombinant -
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种属
Streptococcus -
序列
L DKYGVSDYHK NLINNAKTVE GVKDLQAQVV ESAKKARISE ATDGLSDFLK SQTPAEDTVK SIELAEAKVL ANRELDKYGV SDYYKNLINN AKTVEGVKAL IDEILAALPK TDTYKLILNG KTLKGETTTE AVDAATAEKV FKQYANDNGV DGEWTYDDAT KTFTVTEKPE VIDASELTPA VTTYKLVING KTLK -
预测分子量
63 kDa -
氨基酸
190 to 384
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偶联物
HRP
技术指标
Our Abpromise guarantee covers the use of ab7460 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Western blot
Dot blot
ELISA
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
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形式
Lyophilized -
补充说明
No reaction was observed against anti-Protein A. -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
pH: 7.2
Preservative: 0.01% Gentamicin sulphate
Constituents: 1% BSA, 0.424% Potassium phosphate solution, 0.88% Sodium chloride -
复溶Reconstitute product with 1ml of deionized water.
常规信息
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别名
- IgG binding protein G
- IgG-binding protein G
- Immunoglobulin G binding protein G [Precursor]
see all -
相关性
Protein G is a bacterial protein derived from the cell wall of certain strains of b-hemolytic Streptococcci. It binds with high affinity to the Fc portion of various classes and subclasses of immunoglobulins from a variety of species. Protein G binds to all IgG subclasses from human, mouse and rat species. It also binds to total IgG from guinea pig, rabbit, goat, cow, sheep, and horse. Protein G binds preferentially to the Fc portion of IgG, but can also bind to the Fab region, making it useful for purification of F(ab') fragments of IgG. Due to it's affinity for the Fc region of many mammalian immunoglobulins, protein G is considered a universal reagent in biochemistry and immunology. -
细胞定位
Cell Wall and Secreted
图片
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Western blot - Recombinant Protein G (HRP) (ab7460)Image courtesy of an anonymous Abreview.Used for detection of primary antibody in WB after IP with the same antibody. In this case, IP was with goat anti HP1-alpha ab77256 on Protein G beads, and detection was with goat anti HP1-alpha ab77256, and Protein G HRP ab7460. Detection was very specific, and eliminated heavy and light chains of denatured antibodies.
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Dot Blot - Recombinant Protein G (HRP) (ab7460)Dot Blot analysis of ab7460 Protein G protein (HRP).
Load: 200ng of ab7460 followed by a 3-fold serial dilution
Antigens:
Row 1: Human IgG
Row 2: Rat IgG
Row 3: Dog IgG
Primary antibody: none
Secondary antibody: Protein G Peroxidase Conjugated at 1/1000 for 60 min at room temperature
Block: Blocking buffer for 60 min at room temperature
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (11)
ab7460 被引用在 11 文献中.
- Lee J et al. Abnormal triaging of misfolded proteins by adult neuronal ceroid lipofuscinosis-associated DNAJC5/CSPα mutants causes lipofuscin accumulation. Autophagy 19:204-223 (2023). PubMed: 35506243
- Wu F et al. Acetylation-dependent coupling between G6PD activity and apoptotic signaling. Nat Commun 14:6208 (2023). PubMed: 37798264
- Ng L et al. Celastrol Downmodulates Alpha-Synuclein-Specific T Cell Responses by Mediating Antigen Trafficking in Dendritic Cells. Front Immunol 13:833515 (2022). PubMed: 35309340
- Huettner I et al. Cross-reactivity of glycan-reactive HIV-1 broadly neutralizing antibodies with parasite glycans. Cell Rep 38:110611 (2022). PubMed: 35354052
- Viard J et al. Chr21 protein-protein interactions: enrichment in proteins involved in intellectual disability, autism, and late-onset Alzheimer's disease. Life Sci Alliance 5:N/A (2022). PubMed: 35914814
