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重组蛋白G (ab7458)

Submit a review Q&A (2)

Key features and details

  • Expression system: Escherichia coli
  • Purity: > 95% SDS-PAGE
  • Suitable for: Conjugation

描述

  • 产品名称

    重组蛋白G
    参阅全部 Protein G 蛋白酶

  • 纯度

    > 95 % SDS-PAGE.
    Chromatographically pure Protein G

  • 表达系统

    Escherichia coli

  • Accession

    P19909

  • 蛋白长度

    Full length protein

  • 无动物成分

    No

  • 性质

    Recombinant

    • 种属

      Streptococcus

    • 预测分子量

      26 kDa

技术指标

Our Abpromise guarantee covers the use of ab7458 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    Conjugation

  • 形式

    Lyophilized

  • 补充说明

    Molecular Weight: 26.1 kDa. Approximately 5 mg of human IgG can be bound by 1 mg of Protein G. Optimal binding of Protein G to antibodies occurs at pH 5-6, with elution at pH 2.5-3. This product was expressed recombinantly in E. coli (non-native).

  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.

  • 复溶
    Reconstitute with 1ml of distilled water to a concentration of 1mg/ml

常规信息

  • 别名

    • IgG binding protein G
    • IgG-binding protein G
    • Immunoglobulin G binding protein G [Precursor]
    • spg
    • spg immunoglobulin G-binding protein
    • SPG2_STRSG
    see all

  • 相关性

    Protein G is a bacterial protein derived from the cell wall of certain strains of b-hemolytic Streptococcci. It binds with high affinity to the Fc portion of various classes and subclasses of immunoglobulins from a variety of species. Protein G binds to all IgG subclasses from human, mouse and rat species. It also binds to total IgG from guinea pig, rabbit, goat, cow, sheep, and horse. Protein G binds preferentially to the Fc portion of IgG, but can also bind to the Fab region, making it useful for purification of F(ab') fragments of IgG. Due to it's affinity for the Fc region of many mammalian immunoglobulins, protein G is considered a universal reagent in biochemistry and immunology.

  • 细胞定位

    Cell Wall and Secreted

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (0)

发表研究结果有使用 ab7458？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab7458 尚未被引用在任何文献中。

客户评价及客户问答

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1-2 of 2 Abreviews or Q&A

Question

- protein G (full length)
- protein G (full length) + His tag ....... ab7458.

- protein A (full length) ....... ab117657.
- protein A (full length) + His tag

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Answer

J'aimerais vous informer que mes collègues responsables de l'intégration des produits travaillent toujours à la recherche d'un collaborateur qui pourrait nous fournir :
- une protéine G (full length sans tag) en complément de la référence ab7458 (protéine G, full length, His tag),
- une protéine A (full length avec His-tag) en complément de la référence ab117657 (protéine A, full length, sans tag)

Je vous contacterai de nouveau avec les informations que mes collègues m'auront fournies.

Merci pour votre patience et bon week-end.

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Question

I am interested in your Protein A antibody (ab7244) however there is some discrepancy in your catalog. Under immunogen it lists Protein A from S aureus, the commonly used gold binding protein that also binds to IgG from several species. Later, under relevance, you describe protein A as a human GPCR. These are two very different proteins as far as I can tell. So which is it? Is this product an immunogen against Protein A from S. aureus or an Orphan GPCR? Please send me a reference where this product was used.

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Answer

Thank you for your enquiry. The immunogen is Protein A from S aureus. This product is an antibody against Protein A from S. aureus. I am sorry but I do not have a reference where this product was used. If a reference becomes available we will certainly publish it on the online datasheet. I hope this information helps, please do not hesitate to contact us if you need any more advice or information,

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