重组小鼠WNK4蛋白(ab206027)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 85% Densitometry
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
描述
-
产品名称
重组小鼠WNK4蛋白 -
生物活性
The specific activity of ab206027 was determined to be 1.5 nmol/min/mg.
-
纯度
> 85 % Densitometry.
Affinity purified. -
表达系统
Baculovirus infected Sf9 cells -
Accession
-
蛋白长度
Protein fragment -
无动物成分
No -
性质
Recombinant -
-
种属
Mouse -
序列
MLAPRNTETGVPMSQTEADLALRPSPALTSTGPTRLGPPPRRVRRFSGKA EPRPRSSRPSRRSSVDLGLLSSWSQPASLLPEPPDPPDSAGPTRSPPSSS KEPPEGTWMGAAPVKAVDSACPELTGSSGGPGSREPPRVPDAAARERRRE QEEKEDTETQAVATSPDGRYLKFDIEIGRGSFKTVYRGLDTDTTVEVAWC ELQTRKLSRAERQRFSEEVEMLKGLQHPNIVRFYDSWKSVLRGQVCIVLV TELMTSGTLKTYLRRFREMKPRVLQRWSRQILRGLHFLHSRVPPILHRDL KCDNVFITGPSGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYD EAVDVYAFGMCMLEMATSEYPYSECQNAAQIYRKVTSGTKPNSFYKVKMP EVKEIIEGCIRTDKNERFTIQDLLAHAFFREERGVHVELAE -
预测分子量
86 kDa including tags -
氨基酸
1 to 441 -
标签
GST tag N-Terminus -
额外的序列信息
NM_175638
-
技术指标
Our Abpromise guarantee covers the use of ab206027 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
应用
SDS-PAGE
Functional Studies
-
形式
Liquid -
Concentration information loading...
制备和贮存
-
稳定性和存储
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
pH: 7.50
Constituents: 0.79% Tris HCl, 0.87% Sodium chloride, 0.31% Glutathione, 0.003% EDTA, 0.004% DTT, 0.002% PMSF, 25% Glycerol (glycerin, glycerine)This product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
-
别名
- PHA 2B
- PHA2B
- PRKWNK 4
see all -
功能
Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Activates SCNN1A, SCNN1B, SCNN1D, SGK1, TRPV5 and TRPV6. Regulates the activity of the thiazide-sensitive Na-Cl cotransporter, SLC12A3, by phosphorylation which appears to prevent membrane trafficking of SLC12A3. Also inhibits the renal K(+) channel, KCNJ1, via a kinase-independent mechanism by which it induces clearance of the protein from the cell surface by clathrin-dependent endocytosis. WNK4 appears to act as a molecular switch that can vary the balance between NaCl reabsorption and K(+) secretion to maintain integrated homeostasis. Phosphorylates NEDD4L. Acts as a scaffold to inhibit SLC4A4 as well as CFTR activities and surface expression, recruits STK39 which mediates the inhibition. -
组织特异性
Expressed in kidney, colon and skin. -
疾病相关
Pseudohypoaldosteronism 2B -
序列相似性
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. WNK subfamily.
Contains 1 protein kinase domain. -
翻译后修饰
Phosphorylated by WNK1 and WNK3.
Ubiquitinated by the BCR(KLHL3) complex, leading to its degradation and increased expression of KCNJ1 at the cell surface. Ubiquitinated by the BCR(KLHL2) complex. -
细胞定位
Cell junction, tight junction. Present exclusively in intercellular junctions in the distal convoluted tubule and in both the cytoplasm and intercellular junctions in the cortical collecting duct. WNK4 is part of the tight junction complex (By similarity). - Information by UniProt
图片
-
Functional Studies - Recombinant mouse WNK4 protein (ab206027)
Sample Kinase Activity Plot. The specific activity of ab206027 was determined to be 1.5 nmol/min/mg.
-
SDS-PAGE - Recombinant mouse WNK4 protein (ab206027)SDS-PAGE analysis of ab206027.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
SDS download
-
Datasheet download
文献 (0)
ab206027 尚未被引用在任何文献中。