重组小鼠S100 alpha蛋白(ab108117)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE, MS
描述
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产品名称
重组小鼠S100 alpha蛋白 -
纯度
> 90 % SDS-PAGE.
ab108117 is purified by using anion-exchange chromatography (DEAE sepharose resin) and gel-filtration chromatography (Sephacryl S-200) with 20mM Tris pH 7.5, 2mM EDTA. -
表达系统
Escherichia coli -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Mouse -
序列
MGSSHHHHHHSSGLVPRGSHMGSELESAMETLINVFHAHSGKEGDKYKLS KKELKDLLQTELSGFLDVQKDADAVDKVMKELDENGDGEVDFKEYVVLVA ALTVACNNFFWETS -
预测分子量
13 kDa including tags -
氨基酸
1 to 94 -
标签
His tag N-Terminus
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相关产品
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Related Products
技术指标
Our Abpromise guarantee covers the use of ab108117 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
Mass Spectrometry
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质谱法
MALDI-TOF -
形式
Liquid -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
pH: 8.00
Constituents: 0.0154% DTT, 0.316% Tris HCl, 30% Glycerol (glycerin, glycerine), 0.58% Sodium chloride
常规信息
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别名
- Bpb
- NEF
- Protein S100-A1
see all -
功能
Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. -
组织特异性
Highly prevalent in heart. Also found in lesser quantities in skeletal muscle and brain. -
序列相似性
Belongs to the S-100 family.
Contains 2 EF-hand domains. -
细胞定位
Cytoplasm. - Information by UniProt
图片
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SDS-PAGE - Recombinant Mouse S100 alpha protein (ab108117)15% SDS-PAGE showing ab108117 at approximately 12.6kDa (3µg).
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab108117 尚未被引用在任何文献中。