重组小鼠RANKL蛋白(Active) (ab307187)
Key features and details
- Expression system: HEK 293 cells
- Purity: >= 95% SDS-PAGE
- Endotoxin level: <= 0.005 Eu/µg
- Active: Yes
- Suitable for: Cell Culture, Functional Studies, HPLC, MS, SDS-PAGE
描述
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产品名称
重组小鼠RANKL蛋白(Active)
参阅全部 RANKL 蛋白酶 -
生物活性
Fully biologically active determined by the dose dependent NF-ΚB activation of mouse RAW 264.7 macrophages overexpressing AP-1 and NF-ΚB inducible SEAP reporter gene. Results obtained with InvivoGen RAW BlueTM cells (Cat code.,raw-sp). ED50 ≤ 207 ng/ml, corresponding to a specific activity of 4.8 x 103 units/mg.
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纯度
>= 95 % SDS-PAGE. -
内毒素水平
<=0.005 Eu/µg -
表达系统
HEK 293 cells -
Accession
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蛋白长度
Protein fragment -
无动物成分
Yes -
无载体
是 -
性质
Recombinant -
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种属
Mouse -
序列
GAPAMMEGSWLDVAQRGKPEAQPFAHLTINAASIPSGSHKVTLSSWYHDR GWAKISNMTLSNGKLRVNQDGFYYLYANICFRHHETSGSVPTDYLQLMVY VVKTSIKIPSSHNLMKGGSTKNWSGNSEFHFYSINVGGFFKLRAGEEISI QVSNPSLLDPDQDATYFGAFKVQDID -
预测分子量
20 kDa -
实际分子量
20 kDa -
分子量信息
Predicted MW is 19539.97 Da (+/-10 Da by ESI-TOF). Observed MW is 19541.33 Da. -
氨基酸
141 to 316
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技术指标
Our Abpromise guarantee covers the use of ab307187 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Cell Culture
Functional Studies
HPLC
Mass Spectrometry
SDS-PAGE
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形式
Lyophilized -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at Room Temperature. Store at Room Temperature.
pH: 7.40
Constituents: 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Monobasic dihydrogen potassium phosphate, 10.26% TrehaloseThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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复溶Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product. Store lyophilized form at room temperature. Reconstitute in phosphate buffered saline, aliquot and store at -80°C for 12 months or +4°C for 1 week. Avoid repeated freeze-thaw.
常规信息
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别名
- CD254
- hRANKL2
- ODF
see all -
功能
Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy. -
组织特异性
Highest in the peripheral lymph nodes, weak in spleen, peripheral blood Leukocytes, bone marrow, heart, placenta, skeletal muscle, stomach and thyroid. -
疾病相关
Defects in TNFSF11 are the cause of osteopetrosis autosomal recessive type 2 (OPTB2) [MIM:259710]; also known as osteoclast-poor osteopetrosis. Osteopetrosis is a rare genetic disease characterized by abnormally dense bone, due to defective resorption of immature bone. The disorder occurs in two forms: a severe autosomal recessive form occurring in utero, infancy, or childhood, and a benign autosomal dominant form occurring in adolescence or adulthood. Autosomal recessive osteopetrosis is usually associated with normal or elevated amount of non-functional osteoclasts. OPTB2 is characterized by paucity of osteoclasts, suggesting a molecular defect in osteoclast development. -
序列相似性
Belongs to the tumor necrosis factor family. -
翻译后修饰
The soluble form of isoform 1 derives from the membrane form by proteolytic processing (By similarity). The cleavage may be catalyzed by ADAM17. -
细胞定位
Cytoplasm; Secreted and Cell membrane. - Information by UniProt
图片
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Biological Activity - Recombinant Mouse RANKL Protein (Active) (ab307187)
Fully biologically active determined by the dose dependent NF-ΚB activation of mouse RAW 264.7 macrophages overexpressing AP-1 and NF-ΚB inducible SEAP reporter gene. Results obtained with InvivoGen RAW Blue™ cells (Cat code.,raw-sp). ED50 ≤ 207 ng/ml, corresponding to a specific activity of 4.8 x 103 units/mg.
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SDS-PAGE - Recombinant Mouse RANKL Protein (Active) (ab307187)SDS-PAGE analysis of ab307187
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Mass Spectrometry - Recombinant Mouse RANKL Protein (Active) (ab307187)Mass determination by ESI-TOF. Predicted MW is 19539.97 Da (+/-10 Da by ESI-TOF). Observed MW is 19541.33 Da.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (0)
ab307187 尚未被引用在任何文献中。
