重组小鼠RANKL蛋白(Active) (ab129136)
Key features and details
- Expression system: Escherichia coli
- Purity: >= 95% SDS-PAGE
- Endotoxin level: <= 1.000 Eu/µg
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies
描述
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产品名称
重组小鼠RANKL蛋白(Active)
参阅全部 RANKL 蛋白酶 -
生物活性
ab129136 activity is measured by its ability to induce osteoclast formation on mouse RAW264.7 cells using a concentration of 5.0-10.0 ng/mL (or ≥ 2.0 x 10^4 units/mg).
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纯度
>= 95 % SDS-PAGE.
UV spectroscopy at 280 nm. Purified using standard chromatographical techniques. -
内毒素水平
<=1.000 Eu/µg -
表达系统
Escherichia coli -
Accession
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蛋白长度
Protein fragment -
无动物成分
No -
性质
Recombinant -
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种属
Mouse -
序列
MPAMMEGSWLD VAQRGKPEAQ PFAHLTINAA SIPSGSHKVT LSSWYHDRGW AKISNMTLSN GKLRVNQDGF YYLYANICFR HHETSGSVPT DYLQLMVYVV KTSIKIPSSH NLMKGGSTKN WSGNSEFHFY SINVGGFFKL RAGEEISIQV SNPSLLDPDQ DATYFGAFKV QDID -
预测分子量
20 kDa -
氨基酸
143 to 316
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技术指标
Our Abpromise guarantee covers the use of ab129136 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
Functional Studies
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形式
Lyophilized -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle. For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA).
Constituents: 0.16% Sodium phosphate, 0.29% Sodium chloride
Lyophilized from a sterile (0.2 micron) filtered aqueous solution.This product is an active protein and may elicit a biological response in vivo, handle with caution.
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复溶Centrifuge vial before opening. Gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/mL, which can be further diluted into other aqueous solutions. Allow several minutes for complete reconstitution.
常规信息
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别名
- CD254
- hRANKL2
- ODF
see all -
功能
Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy. -
组织特异性
Highest in the peripheral lymph nodes, weak in spleen, peripheral blood Leukocytes, bone marrow, heart, placenta, skeletal muscle, stomach and thyroid. -
疾病相关
Defects in TNFSF11 are the cause of osteopetrosis autosomal recessive type 2 (OPTB2) [MIM:259710]; also known as osteoclast-poor osteopetrosis. Osteopetrosis is a rare genetic disease characterized by abnormally dense bone, due to defective resorption of immature bone. The disorder occurs in two forms: a severe autosomal recessive form occurring in utero, infancy, or childhood, and a benign autosomal dominant form occurring in adolescence or adulthood. Autosomal recessive osteopetrosis is usually associated with normal or elevated amount of non-functional osteoclasts. OPTB2 is characterized by paucity of osteoclasts, suggesting a molecular defect in osteoclast development. -
序列相似性
Belongs to the tumor necrosis factor family. -
翻译后修饰
The soluble form of isoform 1 derives from the membrane form by proteolytic processing (By similarity). The cleavage may be catalyzed by ADAM17. -
细胞定位
Cytoplasm; Secreted and Cell membrane. - Information by UniProt
图片
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Functional Studies - Recombinant mouse RANKL protein (Active) (ab129136)
ab129136 induced SEAP Production by RAW-blue TM cells.
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Functional Studies - Recombinant mouse RANKL protein (Active) (ab129136)ab129136 Induced production of TNF alpha from RAW cells.
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SDS-PAGE - Recombinant mouse RANKL protein (Active) (ab129136)1ug of ab129136 in a 4-20% Tris-Glycine gel, stained with Coomassie Blue.
Lane 1: Reducing conditions.
Lane 2: Non-reducing conditions.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (6)
ab129136 被引用在 6 文献中.
- Thompson AL et al. Morphine-induced osteolysis and hypersensitivity is mediated through toll-like receptor-4 in a murine model of metastatic breast cancer. Pain 164:2463-2476 (2023). PubMed: 37326644
- Jeong J et al. Synergistic Effect of Whitlockite Scaffolds Combined with Alendronate to Promote Bone Regeneration. Tissue Eng Regen Med 19:83-92 (2022). PubMed: 34962627
- Liu X et al. Osteoclasts protect bone blood vessels against senescence through the angiogenin/plexin-B2 axis. Nat Commun 12:1832 (2021). PubMed: 33758201
- Lee SS et al. Comprehensive in vitro comparison of cellular and osteogenic response to alternative biomaterials for spinal implants. Mater Sci Eng C Mater Biol Appl 127:112251 (2021). PubMed: 34225890
- Peng Y et al. Glucocorticoids Disrupt Skeletal Angiogenesis Through Transrepression of NF-?B-Mediated Preosteoclast Pdgfb Transcription in Young Mice. J Bone Miner Res 35:1188-1202 (2020). PubMed: 32078184
- Elashiry M et al. Dendritic cell derived exosomes loaded with immunoregulatory cargo reprogram local immune responses and inhibit degenerative bone disease in vivo. J Extracell Vesicles 9:1795362 (2020). PubMed: 32944183
