重组小鼠PTP1B蛋白(ab42574)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: Inhibition Assay
描述
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产品名称
重组小鼠PTP1B蛋白
参阅全部 PTP1B 蛋白酶 -
生物活性
The specific activity of mouse PTP1B was determined using pNPP. Enzyme reaction condition: 20 mM pNPP, 5 min incubation at 30C, 2 ug/ml enzyme.
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纯度
> 95 % SDS-PAGE. -
表达系统
Escherichia coli -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Mouse -
氨基酸
1 to 321 -
标签
GST tag N-Terminus
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技术指标
Our Abpromise guarantee covers the use of ab42574 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Inhibition Assay
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形式
Liquid -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.307% Glutathione, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.395% Tris HCl, 0.05% Tween, 50% Glycerol (glycerin, glycerine), 0.435% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
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别名
- PTP1B
- Non receptor tyrosine phosphatase 1
- Protein phosphotyrosylphosphatase 1B
see all -
功能
May play an important role in CKII- and p60c-src-induced signal transduction cascades. -
序列相似性
Belongs to the protein-tyrosine phosphatase family. Non-receptor class 1 subfamily.
Contains 1 tyrosine-protein phosphatase domain. -
翻译后修饰
Oxidized on Cys-215; the Cys-SOH formed in response to redox signaling reacts with the alpha-amido of the following residue to form a 4-amino-3-isothiazolidinone serine cross-link, triggering a conformational change that inhibits substrate binding and activity. The active site can be restored by reduction. -
细胞定位
Endoplasmic reticulum membrane. - Information by UniProt
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab42574 被引用在 1 文献中.
- Ded L et al. 3D in situ imaging of the female reproductive tract reveals molecular signatures of fertilizing spermatozoa in mice. Elife 9:N/A (2020). PubMed: 33078708