重组小鼠Podoplanin蛋白(ab129032)
Key features and details
- Expression system: Escherichia coli
- Purity: > 98% SDS-PAGE
- Tags: His tag C-Terminus
- Suitable for: SDS-PAGE
描述
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产品名称
重组小鼠Podoplanin蛋白
参阅全部 Podoplanin 蛋白酶 -
纯度
> 98 % SDS-PAGE.
Purity is > 98% by SDS-PAGE and silver stain. -
表达系统
Escherichia coli -
Accession
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蛋白长度
Protein fragment -
无动物成分
No -
性质
Recombinant -
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种属
Mouse -
序列
GTIGVNEDDIVTPGTGDGMVPPGIEDKITTTGATGGLNESTGKAPLVPTQ RERGTKPPLEELSTSATSDHDHREHESTTTVKVVTSHSVDKKTSHPNRDN AGDETQTTDKKDGLPVVTLEHHHHHH -
预测分子量
13 kDa including tags -
氨基酸
23 to 141 -
标签
His tag C-Terminus
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描述
重组小鼠Podoplanin / gp36蛋白
相关产品
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Related Products
技术指标
Our Abpromise guarantee covers the use of ab129032 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
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形式
Lyophilized -
补充说明
Endotoxin level: < 0.1ng per µg of Mouse soluble Podoplanin / gp36. -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. The lyophilized protein is stable for a few weeks at room temperature. Store at -20°C long term. Avoid freeze / thaw cycle.
Constituent: 99% PBS
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复溶We recommend a quick spin followed by reconstitution in water to a concentration of 0.1-1.0 mg/ml.
常规信息
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别名
- Aggrus
- D2-40
- Glycoprotein 36 KD
see all -
功能
May be involved in cell migration and/or actin cytoskeleton organization. When expressed in keratinocytes, induces changes in cell morphology with transfected cells showing an elongated shape, numerous membrane protrusions, major reorganization of the actin cytoskeleton, increased motility and decreased cell adhesion. Required for normal lung cell proliferation and alveolus formation at birth. Induces platelet aggregation. Does not have any effect on folic acid or amino acid transport. Does not function as a water channel or as a regulator of aquaporin-type water channels. -
组织特异性
Highly expressed in placenta, lung, skeletal muscle and brain. Weakly expressed in brain, kidney and liver. In placenta, expressed on the apical plasma membrane of endothelium. In lung, expressed in alveolar epithelium. Up-regulated in colorectal tumors and expressed in 25% of early oral squamous cell carcinomas. -
序列相似性
Belongs to the podoplanin family. -
翻译后修饰
Extensively O-glycosylated. Contains sialic acid residues. O-glycosylation is necessary for platelet aggregation activity.
The N-terminus is blocked. -
细胞定位
Membrane. Cell projection > filopodium membrane. Cell projection > lamellipodium membrane. Cell projection > microvillus membrane. Cell projection > ruffle membrane. Localized to actin-rich microvilli and plasma membrane projections such as filopodia, lamellipodia and ruffles. - Information by UniProt
图片
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SDS-PAGE - Recombinant Mouse Podoplanin protein (ab129032)SDS-PAGE analysis of ab129032.
Sample was loaded in 15% SDS-polyacrylamide gel under reducing conditions and stained with Silver stain.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (0)
ab129032 尚未被引用在任何文献中。
