重组小鼠PDGFR alpha蛋白(ab60755)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 90% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
描述
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产品名称
重组小鼠PDGFR alpha蛋白
参阅全部 PDGFR alpha 蛋白酶 -
生物活性
Specific activity: 14 nmol/min/mg.
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纯度
> 90 % SDS-PAGE.
Purity: >90% as determined by densitometry. Affinity purified. -
表达系统
Baculovirus infected Sf9 cells -
Accession
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蛋白长度
Protein fragment -
无动物成分
No -
性质
Recombinant -
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种属
Mouse -
预测分子量
100 kDa -
氨基酸
550 to 1089 -
标签
GST tag N-Terminus -
额外的序列信息
Recombinant fragment, corresponding to amino acids 550-end (cytoplasmic end) of Mouse PDGF Receptor alpha.
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相关产品
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Substrate reagent
技术指标
Our Abpromise guarantee covers the use of ab60755 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Functional Studies
SDS-PAGE
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形式
Liquid -
补充说明
ab204877 (Poly (4:1 Glu, Tyr) peptide) can be utilized as a substrate for assessing kinase activity
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
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别名
- Alpha-type platelet-derived growth factor receptor
- CD140 antigen-like family member A
- CD140a
see all -
功能
Receptor that binds both PDGFA and PDGFB and has a tyrosine-protein kinase activity. -
组织特异性
Expressed in primary and metastatic colon tumors and in normal colon tissue. Tumors may express a different isoform to that found in normal tissue. -
疾病相关
Note=A chromosomal aberration involving PDGFRA is found in some cases of hypereosinophilic syndrome. Interstitial chromosomal deletion del(4)(q12q12) causes the fusion of FIP1L1 and PDGFRA (FIP1L1-PDGFRA). -
序列相似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain. -
细胞定位
Membrane. - Information by UniProt
图片
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Functional Studies - Recombinant mouse PDGFR alpha protein (ab60755)The specific activity of PDGFR alpha (ab60755) was determined to be 14 nmol/min/mg as per activity assay protocol
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SDS-PAGE - Recombinant mouse PDGFR alpha protein (ab60755)SDS PAGE analysis of ab60755 -
Functional Studies - Recombinant mouse PDGFR alpha protein (ab60755)Sample Kinase Activity Plot. -
SDS-PAGE - Recombinant mouse PDGFR alpha protein (ab60755)ab60755 on SDS-PAGE, MW ~100kDa.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab60755 尚未被引用在任何文献中。