重组小鼠PDGF B蛋白(Active) (ab307483)
Key features and details
- Expression system: HEK 293 cells
- Purity: >= 95% HPLC
- Endotoxin level: <= 0.005 Eu/µg
- Active: Yes
- Suitable for: HPLC, SDS-PAGE, MS
描述
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产品名称
重组小鼠PDGF B蛋白(Active)
参阅全部 PDGF B 蛋白酶 -
生物活性
Fully biologically active determined by the dose dependent proliferation of Balb/c 3T3 cells. ED50 is ≤ 0.49 ng/ml, corresponding to a specific activity of 2.04 x 106 units/mg.
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纯度
>= 95 % HPLC.
SDS-PAGE >= 95% -
内毒素水平
<=0.005 Eu/µg -
表达系统
HEK 293 cells -
Accession
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蛋白长度
Full length protein -
无动物成分
Yes -
无载体
是 -
性质
Recombinant -
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种属
Mouse -
序列
SLGSLAAAEPAVIAECKTRTEVFQISRNLIDRTNANFLVWPPCVEVQRCS GCCNNRNVQCRASQVQMRPVQVRKIEIVRKKPIFKKATVTLEDHLACKCE TIVTPRPVT -
预测分子量
12 kDa -
实际分子量
12 kDa -
分子量信息
Predicted MW is 12280.4 Da (+/- 10 Da by ESI-TOF). Observed MW is 12280.44 Da. -
氨基酸
82 to 190 -
额外的序列信息
N-terminal glycine (On N-Term). Mature form. Propeptides not included.
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技术指标
Our Abpromise guarantee covers the use of ab307483 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
HPLC
SDS-PAGE
Mass Spectrometry
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形式
Lyophilized -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at Room Temperature. Store at Room Temperature.
pH: 7.40
Constituents: 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Monobasic dihydrogen potassium phosphate, 10.26% TrehaloseThis product is an active protein and may elicit a biological response in vivo, handle with caution.
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复溶Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product. Store lyophilized form at room temperature. Reconstitute in phosphate buffered saline, aliquot and store at -80°C for 12 months or +4°C for 1 week. Avoid repeated freeze-thaw.
常规信息
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别名
- Becaplermin
- c sis
- FLJ12858
see all - Information by UniProt
图片
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Biological Activity - Recombinant Mouse PDGF B Protein (Active) (ab307483)
Fully biologically active determined by the dose dependent proliferation of Balb/c 3T3 cells. ED50 is ≤ 0.49 ng/ml, corresponding to a specific activity of 2.04 x 106 units/mg.
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Mass Spectrometry - Recombinant Mouse PDGF B Protein (ab307483)Mass determination by ESI-TOF. Predicted MW is 12280.4 Da (+/-10 Da by ESI-TOF). Observed MW is 12280.44 Da.
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HPLC - Recombinant Mouse PDGF B Protein (ab307483)HPLC analysis of ab307483
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SDS-PAGE - Recombinant Mouse PDGF B Protein (ab307483)SDS-PAGE analysis of ab307483
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (0)
ab307483 尚未被引用在任何文献中。
