重组小鼠PDE2A蛋白(Active) (ab271675)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 89% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
描述
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产品名称
重组小鼠PDE2A蛋白(Active)
参阅全部 PDE2A 蛋白酶 -
生物活性
Specific Activity: ≥20,000 pmole/min/µg.One unit is defined as the amount of enzyme that will convert 1 pmole of 3′, 5′-cAMP to 5′-AMP per min at 37°C.Assay condition: 10 mM Tris-HCl, pH 7.4, 10 mM MgCl2, 0.1 mg/ml BSA, 0.05% Tween-20, 200 µM cAMP, and serial dilutions of PDE2A at 37ºC for 20 min. Quantified by 5′ nucleotidase cleaving the 5′-AMP product and releasing the phosphate group which is detected by Malachite Green Reagent. -
纯度
>= 89 % SDS-PAGE.
Affinity purified. -
表达系统
Baculovirus infected Sf9 cells -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Mouse -
预测分子量
130 kDa including tags -
氨基酸
2 to 939 -
标签
GST tag N-Terminus
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技术指标
Our Abpromise guarantee covers the use of ab271675 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
Functional Studies
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形式
Liquid -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.63% Tris HCl, 0.64% Sodium chloride, 0.02% Potassium chloride, 0.04% Tween, 0.05% Glutathione, 20% Glycerol (glycerin, glycerine)This product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
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别名
- 5''-cyclic phosphodiesterase
- cGMP dependent 3'5' cyclic phosphodiesterase
- cGMP stimulated phosphodiesterase 1
see all -
功能
Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. -
组织特异性
Expressed in brain and to a lesser extent in heart, placenta, lung, skeletal muscle, kidney and pancreas. -
序列相似性
Belongs to the cyclic nucleotide phosphodiesterase family. PDE2 subfamily.
Contains 2 GAF domains. -
结构域
GAF 1 functions as a dimerization domain, whereas GAF 2 binds cGMP, which causes activation of the catalytic activity of the enzyme. -
细胞定位
Membrane. - Information by UniProt
图片
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Functional Studies - Recombinant mouse PDE2A protein (Active) (ab271675)
Specific activity of ab271675 was ≥20,000 pmole/min/µg.
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SDS-PAGE - Recombinant mouse PDE2A protein (Active) (ab271675)SDS-PAGE analysis of 1.9 µg ab271675.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab271675 尚未被引用在任何文献中。