重组小鼠PDE11A蛋白(Active) (ab271671)
Key features and details
- Expression system: Sf9 cells
- Purity: >= 85% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
描述
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产品名称
重组小鼠PDE11A蛋白(Active) -
生物活性
Specific Activity: ≥ 1,560 pmol/min/µg.Unit Definition: One unit is defined as the amount of enzyme that will convert 1 pmole of 3′, 5′-cAMP to 5′-AMP per min at 37°C. -
纯度
>= 85 % SDS-PAGE.
Affinity purified. -
表达系统
Sf9 cells -
Accession
-
蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Mouse -
预测分子量
131 kDa -
氨基酸
2 to 933 -
标签
GST tag N-Terminus
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技术指标
Our Abpromise guarantee covers the use of ab271671 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
Functional Studies
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形式
Liquid -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.71% Tris HCl, 0.72% Sodium chloride, 0.02% Potassium chloride, 0.61% Glutathione, 10% Glycerol (glycerin, glycerine), 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diolThis product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
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别名
- 5''-cyclic-AMP and -GMP phosphodiesterase 11A
- cAMP and cGMP phosphodiesterase 11A
- cGMP phosphodiesterase 11A
see all -
功能
Plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides cAMP and cGMP. Catalyzes the hydrolysis of both cAMP and cGMP to 5'-AMP and 5'-GMP, respectively. -
组织特异性
Isoform 1 is present in prostate, pituitary, heart and liver. It is however not present in testis nor in penis, suggesting that weak inhibition by Tadalafil (Cialis) is not relevant (at protein level). Isoform 2 may be expressed in testis. Isoform 4 is expressed in adrenal cortex. -
疾病相关
Defects in PDE11A are the cause of primary pigmented nodular adrenocortical disease type 2 (PPNAD2) [MIM:610475]. Primary pigmented nodular adrenocortical disease is a rare bilateral adrenal defect causing ACTH-independent Cushing syndrome. PPNAD2 is characterized by adrenal glands with overall normal size and weight, and multiple small yellow-to-dark brown nodules surrounded by a cortex with a uniform appearance. Microscopically, there are moderate diffuse cortical hyperplasia with mostly nonpigmented nodules, multiple capsular deficits and massive circumscribed and infiltrating extra-adrenal cortical excrescences with micronodules. PPNAD2 leads to Cushing syndrome. -
序列相似性
Belongs to the cyclic nucleotide phosphodiesterase family.
Contains 2 GAF domains. -
结构域
The tandem GAF domains bind cGMP, and regulate enzyme activity. The binding of cGMP leading to stimulate the enzyme activity. -
细胞定位
Cytoplasm > cytosol. - Information by UniProt
图片
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SDS-PAGE - Recombinant mouse PDE11A protein (Active) (ab271671)
SDS-PAGE analysis of ab271671.
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Functional Studies - Recombinant mouse PDE11A protein (Active) (ab271671)Specific activity of ab271671 was ≥ 1,560 pmol/min/µg.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab271671 尚未被引用在任何文献中。