AB39309

重组小鼠 MMP9 蛋白

Name: 重组小鼠MMP9蛋白 (ab39309)| Abcam中文官网
Brand: Abcam Limited
SKU: AB39309
Price: 4312 CNY
Availability: InStock

Recombinant Mouse MMP9 protein

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(7 Publications)

Recombinant Mouse MMP9 protein is a Mouse Full Length protein, expressed in BHK cells, with 95%, suitable for SDS-PAGE, WB, FuncS.

查看别名

Clg4b, Mmp9, Matrix metalloproteinase-9, MMP-9, 92 kDa gelatinase, 92 kDa type IV collagenase, Gelatinase B, GELB

2 Images

Lab

Western blot - Recombinant Mouse MMP9 protein (AB39309)

This blot was produced using a 4-12% Bis-Tris gel under the MOPS buffer system. The gel was run at 200V for 60 minutes before being transferred onto a nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour before being incubated with anti-MMP9 antibody (ab38898; 2 microgram per mL) overnight at 4°C. Antibody binding was detected using infrared labelled goat anti-rabbit (green) antibody (diluted 1 : 20000) for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-MMP9 antibody (<a href='/products/primary-antibodies/mmp9-antibody-ab38898'>ab38898</a>) at 2 µg/mL

Lane 1:

Western blot - Native human MMP9 protein (Proenzyme, monomer) (<a href='/products/proteins-peptides/native-human-mmp9-protein-proenzyme-monomer-ab157344'>ab157344</a>) at 0.1 µg

Lane 2:

Western blot - Recombinant Mouse MMP9 protein (ab39309) at 0.1 µg

Predicted band size: 78 kDa

false

Lab

Western blot - Recombinant Mouse MMP9 protein (AB39309)

This blot was produced using a 4-12% Bis-Tris gel under the MOPS buffer system. The gel was run at 200V for 60 minutes before being transferred onto a nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour before being incubated with anti-MMP9 antibody [EP1254] (ab76003; 5 microgram per mL) overnight at 4°C. Antibody binding was detected using infrared labeled goat anti-rabbit (green) antibody (diluted 1 : 20000) for 1 hour at room temperature before imaging.

Lanes 1 - 2:

Western blot - Anti-MMP9 antibody [EP1254] (<a href='/products/primary-antibodies/mmp9-antibody-ep1254-ab76003'>ab76003</a>) at 5 µg

Lane 3:

Western blot - Anti-MMP9 antibody [EP1254] (<a href='/products/primary-antibodies/mmp9-antibody-ep1254-ab76003'>ab76003</a>) at 5 µg/mL

Lane 1:

Western blot - Native human MMP9 protein (dimer) (<a href='/products/proteins-peptides/native-human-mmp9-protein-dimer-ab168863'>ab168863</a>) at 0.1 µg

Lane 2:

Western blot - Native human MMP9 protein (Proenzyme, monomer) (<a href='/products/proteins-peptides/native-human-mmp9-protein-proenzyme-monomer-ab157344'>ab157344</a>) at 0.1 µg

Lane 3:

Western blot - Recombinant Mouse MMP9 protein (ab39309) at 0.1 µg

Secondary

All lanes:

Infrared labeled goat anti-rabbit (green) antibody at 1/20000 dilution

Predicted band size: 78 kDa

false

关键信息

纯度

95%

表达系统

BHK cells

应用

WB, SDS-PAGE, FuncS

applications

生物活性

访问

P41245

不含动物源

不含载体蛋白

种属

Mouse

存储溶液

pH: 7.4 Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1.45% Sodium chloride

storage-buffer

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"For Gelatin zymography the MMP9 does not need to be pre-activated, the SDS in the sample buffer will activate the enzyme sufficiently. For any other assays, or to get maximum activity the MMP9 will need activation, as is is mostly in latent form. APMA at 1 mM works, in a buffer of PBS with 10 mM CaCl2, 100 μM ZnCl2, and 0.05% Brij-35, 37°C for a minimum of 1 hour." } } }

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产品详情

Mouse-MMP9 degrades denatured collagen (gelatin), and a range of extracellular matrix components in-vivo. Unlike MMP2, MMP9 is not constituitively produced by most normal cells, although the enzyme is often over expressed by transformed cells, tumor cells, and cells treated with the phorbol ester PMA. An endogenous inhibitor to MMP9, TIMP1, is often complexed with the enzyme in-vivo, but has been removed from this preparation.

ab39309 is a mix of active and latent enzymes, approximately 25% active.

For Gelatin zymography the MMP9 does not need to be pre-activated, the SDS in the sample buffer will activate the enzyme sufficiently. For any other assays, or to get maximum activity the MMP9 will need activation, as is is mostly in latent form. APMA at 1 mM works, in a buffer of PBS with 10 mM CaCl₂, 100 μM ZnCl₂, and 0.05% Brij-35, 37°C for a minimum of 1 hour.

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序列信息

[{"sequence":"","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":null,"accessionNumber":"P41245","tags":[]}]

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性能和储存信息

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

False

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The MMP-9 protein also known as matrix metalloproteinase-9 or gelatinase B functions as an enzyme involved in the breakdown of extracellular matrix proteins. It consists of a zinc ion at its active site and facilitates the degradation of type IV and V collagens fibronectin and elastin. MMP-9 has a molecular weight of approximately 92 kDa and is expressed in a variety of tissues including the liver lungs and immune cells. This enzyme can exist in monomer and dimer forms with its activity regulated by tissue inhibitors of metalloproteinases (TIMPs).

Biological function summary

Matrix metalloproteinase-9 profoundly influences tissue remodeling inflammation and angiogenesis. It does not form part of a larger protein complex but interacts closely with substrates in the extracellular matrix. MMP-9 plays important roles in processes such as embryogenesis reproduction and wound healing through the remodeling of surrounding tissues. The MMP-9 protein is often measured using assays like ELISA or analyzed via techniques such as Western blotting to determine its expression levels and activity in different biological contexts.

Pathways

MMP-9 participates in the matrix metalloproteinase pathway and the NF-kB signaling pathway. These pathways are essential in orchestrating processes such as tissue remodeling and inflammatory responses. MMP-9 acts alongside other matrix metalloproteinases including MMP-2 and is regulated by factors like cytokines growth factors and hormones ensuring the precision of extracellular matrix degradation during physiological processes.

Matrix metalloproteinase-9 has associations with cancer invasion and metastasis as well as chronic inflammatory diseases like rheumatoid arthritis. In cancer upregulated MMP-9 expression aids tumor cells in breaching the basement membrane facilitating metastasis. The disorder-associated pathways often involve interactions with proteins like vascular endothelial growth factor (VEGF) and transforming growth factor-beta (TGF-beta). Understanding MMP-9's role in these conditions can contribute to developing targeted therapies that inhibit its activity potentially mitigating disease progression.

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特殊说明

形式

Liquid

附加说明

Purified from cell culture media of BHK cells, free of its endogenous inhibitor, TIMP1, and other matrix metalloproteinases.

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常规信息

功能

Matrix metalloproteinase that plays an essential role in local proteolysis of the extracellular matrix and in leukocyte migration (By similarity). Could play a role in bone osteoclastic resorption (PubMed : 8132709). Cleaves KiSS1 at a Gly-|-Leu bond (By similarity). Cleaves NINJ1 to generate the Secreted ninjurin-1 form (PubMed : 23142597, PubMed : 32883094). Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide (By similarity).

序列相似性

Belongs to the peptidase M10A family.

翻译后修饰

N- and O-glycosylated.

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产品实验方案

Visit the General protocols
Visit the Troubleshooting

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靶点信息

See full target information Mmp9

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文献 (7)

Recent publications for all applications. Explore the full list and refine your search

Journal of veterinary internal medicine 38:2237-2248 PubMed38842297

2024

Blood-brain barrier dysfunction and decreased transcription of tight junction proteins in epileptic dogs.

Applications

Unspecified application

Species

Unspecified reactive species

Erez Hanael,Shelly Baruch,Rotem Kalev Altman,Orit Chai,Kira Rapoport,Dana Peery,Alon Friedman,Merav H Shamir

Cancer immunology, immunotherapy : CII 72:3079-3095 PubMed37351605

2023

TGF-β1 mediates tumor immunosuppression aggravating at the late stage post-high-light-dose photodynamic therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Lingfei Han,Xiaoxian Huang,Bin Zhao,Hongtan Zhu,Ruyi Wang,Shaoxia Liu,Honglei Lin,Feng Feng,Xiao Ma,Fulei Liu,Jingwei Xue,Wenyuan Liu

Science (New York, N.Y.) 379:eabn8934 PubMed36701450

2023

Low protease activity in B cell follicles promotes retention of intact antigens after immunization.

Applications

Unspecified application

Species

Unspecified reactive species

Aereas Aung,Ang Cui,Laura Maiorino,Ava P Amini,Justin R Gregory,Maurice Bukenya,Yiming Zhang,Heya Lee,Christopher A Cottrell,Duncan M Morgan,Murillo Silva,Heikyung Suh,Jesse D Kirkpatrick,Parastoo Amlashi,Tanaka Remba,Leah M Froehle,Shuhao Xiao,Wuhbet Abraham,Josetta Adams,J Christopher Love,Phillip Huyett,Douglas S Kwon,Nir Hacohen,William R Schief,Sangeeta N Bhatia,Darrell J Irvine

Scientific reports 10:17308 PubMed33057053

2020

From stress to depression: development of extracellular matrix-dependent cognitive impairment following social stress.

Applications

Unspecified application

Species

Unspecified reactive species

Maija-Kreetta Koskinen,Yvar van Mourik,August Benjamin Smit,Danai Riga,Sabine Spijker

PloS one 12:e0185811 PubMed28968441

2017

R1R2 peptide ameliorates pulmonary fibrosis in mice through fibrocyte migration and differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Hou-Yu Chiang,Pao-Hsien Chu,Ting-Hein Lee

American journal of physiology. Gastrointestinal a 309:G988-97 PubMed26514773

2015

Matrix metalloproteinase 9-induced increase in intestinal epithelial tight junction permeability contributes to the severity of experimental DSS colitis.

Applications

Unspecified application

Species

Unspecified reactive species

Prashant Nighot,Rana Al-Sadi,Manmeet Rawat,Shuhong Guo,D Martin Watterson,Thomas Ma

Experimental cell research 314:11-24 PubMed17678891

2007

Tissue inhibitor of metalloproteinase-2 (TIMP-2) regulates myogenesis and beta1 integrin expression in vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Gentian Lluri,Garret D Langlois,Paul D Soloway,Diane M Jaworski

View all publications

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重组小鼠 MMP9 蛋白

Recombinant Mouse MMP9 protein

Western blot - Recombinant Mouse MMP9 protein (AB39309)

Western blot - Recombinant Mouse MMP9 protein (AB39309)

关键信息

纯度

表达系统

标签

应用

生物活性

访问

不含动物源

不含载体蛋白

种属

存储溶液