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AB196418

重组小鼠 MAP3K8/COT 蛋白 (His tag N-Terminus + GST tag N-Terminus)

Recombinant mouse MAP3K8/COT protein (His tag N-Terminus + GST tag N-Terminus)

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Recombinant mouse MAP3K8/COT protein (His tag N-Terminus + GST tag N-Terminus) is a Mouse Fragment protein, in the 30 to 397 aa range, expressed in Baculovirus infected Sf9 cells, with >38%, suitable for SDS-PAGE, FuncS.

查看别名

Cot, Tpl2, Map3k8, Mitogen-activated protein kinase kinase kinase 8, Cancer Osaka thyroid oncogene, Proto-oncogene c-Cot, Serine/threonine-protein kinase cot, Tumor progression locus 2, TPL-2

2 Images
Functional Studies - Recombinant mouse MAP3K8/COT protein (His tag N-Terminus + GST tag N-Terminus) (AB196418)
  • FuncS

Supplier Data

Functional Studies - Recombinant mouse MAP3K8/COT protein (His tag N-Terminus + GST tag N-Terminus) (AB196418)

Activity assay of ab196418.

SDS-PAGE - Recombinant mouse MAP3K8/COT protein (His tag N-Terminus + GST tag N-Terminus) (AB196418)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant mouse MAP3K8/COT protein (His tag N-Terminus + GST tag N-Terminus) (AB196418)

10% SDS-PAGE analysis of 2.5 μg ab196418 with Coomassie staining.

关键信息

纯度

>38% SDS-PAGE

Affinity purified.

表达系统

Baculovirus infected Sf9 cells

标签

His tag N-Terminus GST tag N-Terminus

应用

SDS-PAGE, FuncS

applications

生物活性

Yes

生物学活性

Specific Activity: 8.5 pmole/min/mg.

Reaction was performed in a kinase buffer (40 mM Tris, 10 mM MgCl2, 0.1 mg/ml BSA) containing 5 μg inactive MEK1, 20 μM ATP, and ab196418 for 60 minutes at 30°C. The amount of ATP transferred was measured.

访问

Q07174

不含动物源

No

不含载体蛋白

No

种属

Mouse

存储溶液

pH: 8 Preservative: 0.91% Imidazole Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.15% Glutathione, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.02% Potassium chloride

storage-buffer

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搭配使用产品

Primary Antibodies

AB308011

Anti-MAP3K8/COT antibody [RP23040075]

Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-MAP3K8/COT antibody [RP23040075] (AB308011)

  • 0
  • 0
Primary Antibodies

AB192667

Anti-MAP3K8/COT (phospho S400) antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP3K8/COT (phospho S400) antibody (AB192667)

  • 0
  • 0
Primary Antibodies

AB263947

Anti-STAT6 (phospho Y641) antibody [EPR22599-78]

BOND RX™ Validated|RabMAb|Recombinant|20ul selling size

Flow Cytometry (Intracellular) - Anti-STAT6 (phospho Y641) antibody [EPR22599-78] (AB263947)

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  • 1
Proteins & Peptides

AB287921

Recombinant human Integrin alpha V + beta 3 protein

SDS-PAGE - Recombinant human Integrin alpha V + beta 3 protein (AB287921)

  • 0
  • 0
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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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产品详情

This product was previously labelled as MAP3K8
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序列信息

[{"linker":null,"sequence":"MENLYASEEPGVYEPSLMTMYPDSNQNEERSESLLRSGQEVPWLSSVRYGTVEDLLAFANHVSNMTKHFYGRRPQECGILLNMVISPQNGRYQIDSDVLLVPWKLTYRNIGSGFVPRGAFGKVYLAQDMKTKKRMACKLIPIDQFKPSDVEIQACFRHENIAELYGAVLWGDTVHLFMEAGEGGSVLEKLESCGPMREFEIIWVTKHILKGLDFLHSKKVIHHDIKPSNIVFMSTKAVLVDFGLSVKMTEDVYLPKDLRGTEIYMSPEVILCRGHSTKADIYSLGATLIHMQTGTPPWVKRYPRSAYPSYLYIIHKQAPPLEDIAGDCSPGMRELIEAALERNPNHRPKAADLLKHEALNPPREDQPR","proteinLength":"Fragment","predictedMolecularWeight":"70 kDa","actualMolecularWeight":null,"aminoAcidEnd":397,"aminoAcidStart":30,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"Q07174","tags":[{"tag":"His","terminus":"N-Terminus"},{"tag":"GST","terminus":"N-Terminus"}]}]
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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification
运输条件
Dry Ice
推荐的短期储存条件
-80°C
推荐的长期储存条件
-80°C
储存信息
Avoid freeze / thaw cycle
True
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常规信息

功能

Required for lipopolysaccharide (LPS)-induced, TLR4-mediated activation of the MAPK/ERK pathway in macrophages, thus being critical for production of the pro-inflammatory cytokine TNF (TNF) during immune responses. Involved in the regulation of T-helper cell differentiation and IFNG expression in T-cells. Involved in mediating host resistance to bacterial infection through negative regulation of type I interferon (IFN) production. Transduces CD40 and TNFRSF1A signals that activate ERK in B-cells and macrophages, and thus may play a role in the regulation of immunoglobulin production. May also play a role in the transduction of TNF signals that activate JNK and NF-kappa-B in some cell types. In adipocytes, activates MAPK/ERK pathway in an IKBKB-dependent manner in response to IL1B and TNF, but not insulin, leading to induction of lipolysis. Plays a role in the cell cycle.

序列相似性

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.

翻译后修饰

Autophosphorylated.

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产品实验方案

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靶点信息

Required for lipopolysaccharide (LPS)-induced, TLR4-mediated activation of the MAPK/ERK pathway in macrophages, thus being critical for production of the pro-inflammatory cytokine TNF (TNF) during immune responses. Involved in the regulation of T-helper cell differentiation and IFNG expression in T-cells. Involved in mediating host resistance to bacterial infection through negative regulation of type I interferon (IFN) production. Transduces CD40 and TNFRSF1A signals that activate ERK in B-cells and macrophages, and thus may play a role in the regulation of immunoglobulin production. May also play a role in the transduction of TNF signals that activate JNK and NF-kappa-B in some cell types. In adipocytes, activates MAPK/ERK pathway in an IKBKB-dependent manner in response to IL1B and TNF, but not insulin, leading to induction of lipolysis. Plays a role in the cell cycle.
See full target information Map3k8
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