重组小鼠M-CSF蛋白(ab129146)
Key features and details
- Expression system: Escherichia coli
- Purity: >= 95% SDS-PAGE
- Endotoxin level: <= 1.000 Eu/µg
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies
描述
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产品名称
重组小鼠M-CSF蛋白
参阅全部 M-CSF 蛋白酶 -
生物活性
Method of Determination: NFS-60 cell proliferation
Acception Criteria: ED50 ≤10 ng/mL(≥ 1.0 x 10^5 units/mg)
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纯度
>= 95 % SDS-PAGE.
Purified using standard chromatographical techniques. -
内毒素水平
<=1.000 Eu/µg -
表达系统
Escherichia coli -
Accession
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蛋白长度
Protein fragment -
无动物成分
No -
性质
Recombinant -
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种属
Mouse -
序列
MKEVSEHCSH MIGNGHLKVL QQLIDSQMET SCQIAFEFVD QEQLDDPVCY LKKAFFLVQD IIDETMRFKD NTPNANATER LQELSNNLNS CFTKDYEEQN KACVRTFHET PLQLLEKIKN FFNETKNLLE KDWNIFTKNC NNSFAKCSSR DVVTKPMKEV SEHCSHMIGN GHLKVLQQLI DSQMETSCQI AFEFVDQEQL DDPVCYLKKA FFLVQDIIDE TMRFKDNTPN ANATERLQEL SNNLNSCFTK DYEEQNKACV RTFHETPLQL LEKIKNFFNE TKNLLEKDWN IFTKNCNNSF AKCSSRDVVT KP -
预测分子量
36 kDa -
氨基酸
33 to 187
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技术指标
Our Abpromise guarantee covers the use of ab129146 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
Functional Studies
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形式
Lyophilized -
补充说明
Endotoxin levels determined by kinetic LAL (50% Confidence).
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at Room Temperature. Upon reconsitution add a carrier protein (0.1% BSA). Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 0.16% Sodium phosphate, 0.29% Sodium chloride
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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复溶Sterile water at 0.1 mg/mL Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution.
常规信息
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别名
- Colony stimulating factor 1
- Colony stimulating factor 1 (macrophage)
- Colony stimulating factor macrophage specific
see all -
功能
Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. CSF-1 induces cells of the monocyte/macrophage lineage. It plays a role in immunological defenses, bone metabolism, lipoproteins clearance, fertility and pregnancy. -
翻译后修饰
Glycosylation and proteolytic cleavage yield different soluble forms. A high molecular weight soluble form is a proteoglycan containing chondroitin sulfate.
Isoform 1 is N- and O-glycosylated. Isoform 3 is N-glycosylated. -
细胞定位
Cell membrane and Secreted > extracellular space. - Information by UniProt
图片
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (5)
ab129146 被引用在 5 文献中.
- Bresque M et al. SIRT6 stabilization and cytoplasmic localization in macrophages regulates acute and chronic inflammation in mice. J Biol Chem 298:101711 (2022). PubMed: 35150745
- Gao Z et al. Nociceptor Neurons are Involved in the Host Response to Escherichia coli Urinary Tract Infections. J Inflamm Res 15:3337-3353 (2022). PubMed: 35702548
- Huang C et al. Hepatocyte growth factor overexpression promotes osteoclastogenesis and exacerbates bone loss in CIA mice. J Orthop Translat 27:9-16 (2021). PubMed: 33344167
- Wang R et al. Urinary Trypsin Inhibitor Protects Tight Junctions of Septic Pulmonary Capillary Endothelial Cells by Regulating the Functions of Macrophages. J Inflamm Res 14:1973-1989 (2021). PubMed: 34045879
- Zheng Y et al. Gentiana scabra Restrains Hepatic Pro-Inflammatory Macrophages to Ameliorate Non-Alcoholic Fatty Liver Disease. Front Pharmacol 12:816032 (2021). PubMed: 35115947