重组小鼠JNK3蛋白(ab119734)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 80% SDS-PAGE
- Active: Yes
- Suitable for: WB, Functional Studies, SDS-PAGE
描述
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产品名称
重组小鼠JNK3蛋白
参阅全部 JNK3 蛋白酶 -
生物活性
Specific Activity: 35 nmol/min/mg
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纯度
> 80 % SDS-PAGE.
Purity determined to be >80% by densitometry. Affinity purified. -
表达系统
Baculovirus infected Sf9 cells -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Mouse -
预测分子量
71 kDa including tags -
氨基酸
1 to 464
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技术指标
Our Abpromise guarantee covers the use of ab119734 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Western blot
Functional Studies
SDS-PAGE
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形式
Liquid -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol (glycerin, glycerine), 0.88% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
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别名
- c Jun kinase 3
- c-Jun N-terminal kinase 3
- cJun N terminal kinase 3
see all -
功能
Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2 and thus regulates AP-1 transcriptional activity. Required for stress-induced neuronal apoptosis and the pathogenesis of glutamate excitotoxicity. -
组织特异性
Specific to a subset of neurons in the nervous system. Present in the hippocampus and areas, cerebellum, striatum, brain stem, and weakly in the spinal cord. Very weak expression in testis and kidney. -
疾病相关
Defects in MAPK10 are a cause of epileptic encephalopathy Lennox-Gastaut type (EELG) [MIM:606369]. Epileptic encephalopathies of the Lennox-Gastaut group are childhood epileptic disorders characterized by severe psychomotor delay and seizures. Note=A chromosomal aberration involving MAPK10 has been found in a single patient. Translocation t(Y;4)(q11.2;q21) which causes MAPK10 truncation. -
序列相似性
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain. -
结构域
The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases. -
翻译后修饰
Dually phosphorylated on Thr-221 and Tyr-223, which activates the enzyme. Weakly autophosphorylated on threonine and tyrosine residues in vitro. -
细胞定位
Cytoplasm. - Information by UniProt
图片
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Functional Studies - Recombinant mouse JNK3 protein (ab119734)The specific activity of JNK3 (ab119734) was determined to be 30 nmol/min/mg as per activity assay protocol
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SDS-PAGE - Recombinant mouse JNK3 protein (ab119734)SDS PAGE analysis of ab119734 -
SDS-PAGE - Recombinant mouse JNK3 protein (ab119734)SDS PAGE analysis of ab119734 -
Functional Studies - Recombinant mouse JNK3 protein (ab119734)The specific activity of ab119734 was determined to be 35 nmol/min/mg. -
SDS-PAGE - Recombinant mouse JNK3 protein (ab119734)SDS-PAGE analysis of ab119374.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab119734 尚未被引用在任何文献中。