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AB280343

重组小鼠IL-21蛋白(Active)

Recombinant Mouse IL-21 protein (Active)

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Recombinant Mouse IL-21 protein (Active) is a Mouse Full Length protein, in the 18 to 146 aa range, expressed in HEK 293 cells, with >95%, <0.005 EU/µg endotoxin level, suitable for SDS-PAGE, Mass Spec, HPLC, Cell Culture, FuncS.

查看别名

Interleukin-21, IL-21, Il21

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Functional Studies - Recombinant Mouse IL-21 protein (Active) (AB280343)
  • FuncS

Unknown

Functional Studies - Recombinant Mouse IL-21 protein (Active) (AB280343)

Fully biological active determined by its ability to enhance IFNɣ secretion in presence of human IL-15 in human KHYG-1 cells. ED50 for this effect is ≤7.9 ng/ml, corresponding to a specific activity of 1.25x105 units/mg.

Cell based assay testing is performed on the first lot of protein only and is provided as a reference for protein activity; subsequent lots of protein must pass all biophysical quality control parameters that meet the same parameters as the first lot.

Lot GR3380830-2

Mass Spectrometry - Recombinant Mouse IL-21 protein (Active) (AB280343)
  • Mass Spec

Supplier Data

Mass Spectrometry - Recombinant Mouse IL-21 protein (Active) (AB280343)

Mass determination by ESI-TOF.

Predicted MW is 15021.53 Da (+/- 10 Da by ESI-TOF). Observed mass is 15021.80 Da.

HPLC - Recombinant Mouse IL-21 protein (Active) (AB280343)
  • HPLC

Supplier Data

HPLC - Recombinant Mouse IL-21 protein (Active) (AB280343)

HPLC analysis of ab280343.

SDS-PAGE - Recombinant Mouse IL-21 protein (Active) (AB280343)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Mouse IL-21 protein (Active) (AB280343)

SDS-PAGE analysis of ab280343.

关键信息

纯度

>95% SDS-PAGE

内毒素水平

<0.005 EU/µg

表达系统

HEK 293 cells

标签

Tag free

应用

FuncS, Cell Culture, HPLC, SDS-PAGE, Mass Spec

applications

生物活性

Yes

生物学活性

Fully biological active determined by its ability to enhance IFNɣ secretion in presence of human IL-15 in human KHYG-1 cells. ED50 for this effect is ≤7.9 ng/ml, corresponding to a specific activity of 1.25x105 units/mg.

访问

Q9ES17

不含动物源

Yes

不含载体蛋白

No

种属

Mouse

复溶

Reconstitute in PBS

存储溶液

pH: 7.4 Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

storage-buffer

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Cell Culture": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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序列信息

[{"linker":null,"sequence":"HKSSPQGPDRLLIRLRHLIDIVEQLKIYENDLDPELLSAPQDVKGHCEHAAFACFQKAKLKPSNPGNNKTFIIDLVAQLRRRLPARRGGKKQKHIAKCPSCDSYEKRTPKEFLERLKWLLQKMIHQHLS","proteinLength":"Full Length","predictedMolecularWeight":"15.02 kDa","actualMolecularWeight":"15.02 kDa","aminoAcidEnd":146,"aminoAcidStart":18,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"Q9ES17","tags":[]}]
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性能和储存信息

运输条件
Ambient - Can Ship with Ice
推荐的短期储存条件
Ambient
推荐的长期储存条件
Ambient
True
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特殊说明

形式

Lyophilized

附加说明

=95%  by HPLC

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常规信息

功能

Cytokine with immunoregulatory activity. May promote the transition between innate and adaptive immunity. Induces the production of IgG(1) and IgG(3) in B-cells (PubMed : 15100251). Implicated in the generation and maintenance of T follicular helper (Tfh) cells and the formation of germinal-centers. Together with IL6, control the early generation of Tfh cells and are critical for an effective antibody response to acute viral infection (PubMed : 23045607). May play a role in proliferation and maturation of natural killer (NK) cells in synergy with IL15 (PubMed : 11970879). May regulate proliferation of mature B- and T-cells in response to activating stimuli. In synergy with IL15 and IL18 stimulates interferon gamma production in T-cells and NK cells (By similarity). During T-cell mediated immune response may inhibit dendritic cells (DC) activation and maturation (PubMed : 12893770).

序列相似性

Belongs to the IL-15/IL-21 family.

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产品实验方案

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靶点信息

Cytokine with immunoregulatory activity. May promote the transition between innate and adaptive immunity. Induces the production of IgG(1) and IgG(3) in B-cells (PubMed : 15100251). Implicated in the generation and maintenance of T follicular helper (Tfh) cells and the formation of germinal-centers. Together with IL6, control the early generation of Tfh cells and are critical for an effective antibody response to acute viral infection (PubMed : 23045607). May play a role in proliferation and maturation of natural killer (NK) cells in synergy with IL15 (PubMed : 11970879). May regulate proliferation of mature B- and T-cells in response to activating stimuli. In synergy with IL15 and IL18 stimulates interferon gamma production in T-cells and NK cells (By similarity). During T-cell mediated immune response may inhibit dendritic cells (DC) activation and maturation (PubMed : 12893770).
See full target information Il21
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