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AB270071

重组小鼠Flt3 ligand/Flt3L蛋白(Active)

Recombinant mouse Flt3 ligand/Flt3L protein (Active)

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(1 Publication)

Recombinant mouse Flt3 ligand/Flt3L protein (Active) is a Mouse Fragment protein, in the 27 to 189 aa range, expressed in HEK 293 cells, with >95%, <0.005 EU/µg endotoxin level, suitable for SDS-PAGE, FuncS, Mass Spec, HPLC, Cell Culture.

查看别名

Flt3l, Flt3lg, Fms-related tyrosine kinase 3 ligand, Flt3 ligand, Flt3L, SL cytokine

4 Images
Functional Studies - Recombinant mouse Flt3 ligand/Flt3L protein (Active) (AB270071)
  • FuncS

Supplier Data

Functional Studies - Recombinant mouse Flt3 ligand/Flt3L protein (Active) (AB270071)

Fully biologically active determined by the dose dependent proliferation of AML-5 cells.

ED50 is ≤2.54 ng/ml, corresponding to a specific activity of 0.39 x 106 units/mg.

Cell based assay testing is performed on the first lot of protein only and is provided as a reference for protein activity; subsequent lots of protein must pass all biophysical quality control parameters that meet the same parameters as the first lot.

Lot GR3369520-1

Mass Spectrometry - Recombinant mouse Flt3 ligand/Flt3L protein (Active) (AB270071)
  • Mass Spec

Supplier Data

Mass Spectrometry - Recombinant mouse Flt3 ligand/Flt3L protein (Active) (AB270071)

Mass determination by ESI TOF

Predicted MW is 18570.46 +/- 10Da by ESI-TOF. Observed MW is 18571.49. Additional Peaks due to Chains +Gly + O-glycans.

SDS-PAGE - Recombinant mouse Flt3 ligand/Flt3L protein (Active) (AB270071)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant mouse Flt3 ligand/Flt3L protein (Active) (AB270071)

SDS-PAGE analysis of ab270071.

HPLC - Recombinant mouse Flt3 ligand/Flt3L protein (Active) (AB270071)
  • HPLC

Supplier Data

HPLC - Recombinant mouse Flt3 ligand/Flt3L protein (Active) (AB270071)

HPLC analysis of ab270071.

Additional peaks due to glycosylation.

关键信息

纯度

>95% SDS-PAGE

=95%  by HPLC

内毒素水平

<0.005 EU/µg

表达系统

HEK 293 cells

标签

Tag free

应用

SDS-PAGE, Mass Spec, Cell Culture, FuncS, HPLC

applications

生物活性

Yes

生物学活性

Fully biologically active determined by the dose dependent proliferation of AML-5 cells.

ED50 is ≤2.54 ng/ml, corresponding to a specific activity of 0.39 x 106 units/mg.

访问

P49772

不含动物源

Yes

不含载体蛋白

No

种属

Mouse

复溶

Reconstitute in PBS

存储溶液

Constituents: 10.26% Trehalose, 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Potassium phosphate monobasic

storage-buffer

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搭配使用产品

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Cell Culture": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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序列信息

[{"linker":null,"sequence":"GTPDCYFSHSPISSNFKVKFRELTDHLLKDYPVTVAVNLQDEKHCKALWSLFLAQRWIEQLKTVAGSKMQTLLEDVNTEIHFVTSCTFQPLPECLRFVQTNISHLLKDTCTQLLALKPCIGKACQNFSRCLEVQCQPDSSTLLPPRSPIALEATELPEPRPRQ","proteinLength":"Fragment","predictedMolecularWeight":"18.57 kDa","actualMolecularWeight":"18.57 kDa","aminoAcidEnd":189,"aminoAcidStart":27,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P49772","tags":[]}]
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性能和储存信息

形式
Lyophilized
运输条件
Ambient - Can Ship with Ice
推荐的短期储存条件
Ambient
推荐的长期储存条件
Ambient
True
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The Flt3 ligand (Flt3L) also known as Flt3-L Flt3 ligand protein or flt-3 ligand protein is a small cytokine with a molecular mass of approximately 18 to 19 kDa. It acts as a growth factor by binding to the Flt3 receptor tyrosine kinase on the surface of hematopoietic progenitor cells. Flt3L is expressed in various tissues including the bone marrow where it plays a significant role in hematopoiesis. It is critical in dendritic cell and B cell development impacting the immune response mechanism.
Biological function summary

The Flt3 ligand plays an essential role in the immune system. It is a monomeric cytokine that induces proliferation of early lineage cells within the hematopoietic system. Flt3L does not function as part of a complex but acts independently to stimulate the proliferation differentiation and survival of hematopoietic cells. By interacting directly with its receptor it significantly influences the innate and adaptive immune systems through the maturation of dendritic and B cells.

Pathways

The Flt3 ligand is involved in hematopoietic signaling pathways primarily the PI3K-Akt signaling pathway. This pathway is essential for cell survival and growth where Flt3L aids in transmitting growth signals necessary for hematopoietic cells. Flt3L also interacts with proteins such as FLT3 receptor and influences the downstream signaling cascade affecting cell fate decisions in hematopoiesis and immune cell maturation.

Flt3 ligand is connected to leukemia particularly acute myeloid leukemia (AML) due to dysregulation of the FLT3 signaling pathway. Mutations in the FLT3 receptor can lead to uncontrolled cell proliferation contributing to tumorigenesis. Additionally Flt3L plays a role in autoimmune disorders by influencing immune cell development. It is closely connected with the FLT3 receptor and other tyrosine kinases which when mutated can exacerbate disease pathology through abnormal cell signaling and proliferation.
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常规信息

功能

Stimulates the proliferation of early hematopoietic cells by activating FLT3. Synergizes well with a number of other colony stimulating factors and interleukins. Essential for the development of natural killer (NK) cells, B cells, and dendritic cells (DCs).

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产品实验方案

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靶点信息

Stimulates the proliferation of early hematopoietic cells by activating FLT3. Synergizes well with a number of other colony stimulating factors and interleukins. Essential for the development of natural killer (NK) cells, B cells, and dendritic cells (DCs).
See full target information Flt3lg
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Gastroenterology 163:411-425.e4 PubMed35487288

2022

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Applications

Unspecified application

Species

Unspecified reactive species

Lin Ding,Jayati Chakrabarti,Sulaiman Sheriff,Qian Li,Hahn Nguyen Thi Hong,Ricky A Sontz,Zoe E Mendoza,Amanda Schreibeis,Michael A Helmrath,Yana Zavros,Juanita L Merchant
View all publications
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