重组小鼠FGF2蛋白(ab129033)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE
描述
-
产品名称
重组小鼠FGF2蛋白
参阅全部 FGF2 蛋白酶 -
生物活性
The activity was determined by the stimulation of HUVE cells. The ED50 for this effect is = 0.1ng/ml corresponding to a specific activity of = 1 x 107 units/mg.
-
纯度
> 95 % SDS-PAGE. -
表达系统
Escherichia coli -
Accession
-
蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
-
种属
Mouse -
序列
ALPEDGGAAFPPGHFKDPKRLYCKNGGFFLRIHPDGRVDGVREKSDPHVK LQLQAEERGVVSIKGVCANRYLAMKEDGRLLASKCVTEECFFFERLESNN YNTYRSRKYSSWYVALKRTGQYKLGSKTGPGQKAILFLPMSAKS -
氨基酸
11 to 154
-
技术指标
Our Abpromise guarantee covers the use of ab129033 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
应用
Functional Studies
SDS-PAGE
-
形式
Lyophilized -
Concentration information loading...
制备和贮存
-
稳定性和存储
Shipped at 4°C. The lyophilized protein is stable for a few weeks at room temperature. Store at -20°C long term.
Constituent: 99% PBS
This product is an active protein and may elicit a biological response in vivo, handle with caution.
-
复溶ab129033 can be reconstituted with ddH2O or 50 mM acedic acid at 50 µg/mL. This solution can be diluted into other buffered solutions or stored frozen for future use. Avoid repeated freeze-thaw cycles.
常规信息
-
别名
- Basic fibroblast growth factor
- Basic fibroblast growth factor bFGF
- BFGF
see all -
功能
Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Can induce angiogenesis (PubMed:23469107). -
组织特异性
Expressed in granulosa and cumulus cells. Expressed in hepatocellular carcinoma cells, but not in non-cancerous liver tissue. -
序列相似性
Belongs to the heparin-binding growth factors family. -
翻译后修饰
Phosphorylation at Tyr-215 regulates FGF2 unconventional secretion.
Several N-termini starting at positions 94, 125, 126, 132, 143 and 162 have been identified by direct sequencing. -
细胞定位
Secreted. Nucleus. Exported from cells by an endoplasmic reticulum (ER)/Golgi-independent mechanism. Unconventional secretion of FGF2 occurs by direct translocation across the plasma membrane. Binding of exogenous FGF2 to FGFR facilitates endocytosis followed by translocation of FGF2 across endosomal membrane into the cytosol. Nuclear import from the cytosol requires the classical nuclear import machinery, involving proteins KPNA1 and KPNB1, as well as CEP57. - Information by UniProt
图片
-
SDS-PAGE - Recombinant mouse FGF2 protein (ab129033)15% SDS-PAGE analysis of ab129033 with subsequent Silver staining.
-
Functional Studies - Recombinant mouse FGF2 protein (ab129033)Dose-dependent stimulation of cell proliferation in HUVE cells by recombinant rat FGF2 and ab129033 (mFGF2). Values are the means (±SD) of triplicate determinations and expressed as percentage of control.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
Datasheet download
文献 (0)
ab129033 尚未被引用在任何文献中。
