重组小鼠ERK2 (mutated K52R)蛋白(ab62568)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Tags: His tag N-Terminus
- Suitable for: Functional Studies
描述
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产品名称
重组小鼠ERK2 (mutated K52R)蛋白
参阅全部 ERK2 蛋白酶 -
纯度
> 90 % SDS-PAGE. -
表达系统
Escherichia coli -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Mouse -
预测分子量
42 kDa -
修饰
mutated K52R -
标签
His tag N-Terminus
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相关产品
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Related Products
技术指标
Our Abpromise guarantee covers the use of ab62568 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Functional Studies
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形式
Liquid -
补充说明
The K52 R mutation prevents ATP binding, making the kinase inactive. ab62568 is useful as a substrate for MEK1.
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Preservative: 0.85% Imidazole
Constituents: 0.0462% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.71% Tris HCl, 10% Glycerol (glycerin, glycerine), 0.72% Sodium chloride, 0.02% Potassium chloride
常规信息
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别名
- ERK 2
- ERK-2
- ERT1
see all -
功能
Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4) and ARHGEF2.
Acts as a transcriptional repressor. Binds to a [GC]AAA[GC] consensus sequence. Repress the expression of interferon gamma-induced genes. Seems to bind to the promoter of CCL5, DMP1, IFIH1, IFITM1, IRF7, IRF9, LAMP3, OAS1, OAS2, OAS3 and STAT1. Transcriptional activity is independent of kinase activity. -
序列相似性
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain. -
结构域
The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases. -
翻译后修饰
Dually phosphorylated on Thr-185 and Tyr-187, which activates the enzyme. Dephosphorylated by PTPRJ at Tyr-187. -
细胞定位
Nucleus. - Information by UniProt
图片
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SDS-PAGE - Recombinant Mouse ERK2 (mutated K52R) protein (ab62568)ab62568 on 10% SDS-PAGE, Coomassie staining.
Lane 1 ab62568.
Lane 2 Protein marker.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab62568 尚未被引用在任何文献中。